View our list of available Coronavirus (COVID-19, SARS-CoV-2, 2019-nCOV) antibodies, recombinant proteins, ELISA Kits... Options Full Datasheet Printer Friendly Datasheet Prepare for Printing Full Datasheet Printer Friendly Datasheet Prepare for Printing Have Questions? Ask Us! Request A Quote Today! Add To Compare List Mouse anti-Human CD152, Blocking Monoclonal Antibody Catalog # MBS632798 Unit / Price 0.05 mg / $515 +1 FREE 8GB USB 0.1 mg / $760 +1 FREE 8GB USB 5x0.1 mg / $3,255 +4 FREE 8GB USB Product Name Product Info Accession #s Product Desc Diseases/Tissues/Pathways Applications References Product Name CD152, Blocking, Monoclonal Antibody Popular Item Full Product Name CD152, Blocking (CTLA4, Cytolytic T-lymphocyte-associated Antigen 4) Product Synonym Names Anti -CD152, Blocking (CTLA4, Cytolytic T-lymphocyte-associated Antigen 4) Research Use Only For Research Use Only. Not for use in diagnostic procedures. TOP Clonality Monoclonal Isotype IgG1,k Clone Number 5D3 Host Mouse Species Reactivity Human Specificity Recognizes CTLA-4 expressed on activated T cells. Blocks binding of CD152 Human, IgG2a Mouse Fc, Fusion Protein to its CD80/CD86 receptor Purity/Purification Affinity PurifiedPurified by Protein A affinity chromatography from (low FBS containing) tissue culture supernatant. Purity was > 95% immunoglobulin by SDS-PAGE and < 1% bovine Immunoglobulin. Form/Format Supplied as a liquid in PBS, pH 7.5, 0.5mg/ml gentamicin sulfate. Concentration 1 mg/mL (lot specific) Immunogen Stimulated human PBL's and human CD152 Ig fusion protein. Flow Cytometry (Blocking) Protocol MBS632798 Anti-CD152 antibody blocked the binding of MBS635220 CD152 Human, IgG2a Mouse Fc, Fusion Protein (PE) to CD80 and CD86 expressed on human Raji cells. 1. Five x 10e5 cultured Raji human tumor cells were washed and incubated 45 minutes on ice with 80ul of MBS635220 at a concentration of 1ug/ml. . 2. Cells were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 1.79 log 10 fluorescent units when compared to a Mouse IgG2a Isotype control (PE) at a similar concentration. Binding was 98.6% blocked when MBS635220 CD152 Human, IgG2a Mouse Fc,Fusion Protein (PE) was pre incubated 10 minutes with a five fold excess (w/w) of MBS632798CD152, Blocking (5ug/ml Preparation and Storage May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap . Other Notes Small volumes of anti-CD152, Blocking antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply. TOP Related Product Information for anti-CD152, Blocking antibody Human CD152 (CTLA-4) is a cell surface glycoprotein expressed at low levels on activated T-cells. CD152 is a highaffinity receptor for the costimulatory molecules CD80 (B7-1) and CD86 (B7-2) and appears to function as a negative regulator of T cell activation. Product Categories/Family for anti-CD152, Blocking antibody Antibodies; Abs to CD Markers Applications Tested/Suitable for anti-CD152, Blocking antibody Suitable for use in Flow Cytometry, ELISA and Immunohistochemistry. Other applications not tested Application Notes for anti-CD152, Blocking antibody Flow Cytometry (Blocking): 5ug/mlOptimal dilutions to be determined by the researcher. Testing Data of anti-CD152, Blocking antibody TOP Precautions All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines. Disclaimer While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information. TOP