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anti-CD152 antibody :: Mouse CD152 Monoclonal Antibody

Scan QR to view Datasheet Catalog #    MBS666569
Testing Data
Unit / Price
120 Tests  /  $440 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

CD152, Monoclonal Antibody

★Popular Item★
 Also Known As   

anti-human CD152-FITC

 Product Synonym Names    Monoclonal anti-human CD152 (CTLA-4)/FITC
 Product Gene Name   

anti-CD152 antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Clonality    Monoclonal
 Isotype    IgG1k
 Clone Number    ANC152.2/8H5
 Host    Mouse
 Species Reactivity    Human, Pig, Cow, Cat, Dog
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 Form/Format    50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl, 5% Glycerol, 0.2% BSA.
 Concentration    Volume: 0.2ml (lot specific)
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 Performance    Anti-CD152/FITC was tested for binding to CD152-muIg-coated 4u beads in FACS (Inquire). Two x 105 CD152-muIg coated beads per tube were spun down and incubated 45 minutes at 4 degree C with 80ul of anti-CD152/FITC at a 1:50 dilution (10ug/ml). Tubes were washed twice, fixed and analyzed by FACS. Beads stained positive with a mean shift of 2.03 log10 fluorescent units when compared to a Mouse IgG1/FITC Isotype control at a similar concentration. Binding was blocked when beads were pre incubated 10 minutes with 20 ul of 0.5 mg/ml anti-CD152 antibody.
 Conjugation    FITC
 Production    Protein A purified antibody from tissue culture supernatant was reacted with FITC. Unconjugated FITC was separated from antibody/FITC conjugate by desalting column. The antibody/FITC conjugate is at 0.5 mg/ml with a Fluorescein/IgG molar ratio of 6.1.
 Buffer    50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl, 5% Glycerol, 0.2% BSA, 0.04% NaN3 (as a preservative).
 Immunogen    Stimulated human PBL’s and human CD152 Ig fusion protein
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 Preparation and Storage    Store at 2 - 5 degree C. Freeze/Thawing is not recommended. Protect from light
Product should retain activity for at least 12 months after shipping date when stored as recommended.
 Other Notes    Small volumes of anti-CD152 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for anti-CD152 antibody

   Information: Human CD152 (CTLA-4) is a cell surface glycoprotein expressed at low levels on activated T cells. CD152 is a high affinity receptor for the costimulatory molecules CD80 (B7-1) and CD86 (B7-2) and appears to function as a negative regulator of T cell activation. Antibody ANC152.2 reacts with CTLA-4 expressed on activated T cells. ANC152.2 blocks binding of CD152 (CTLA-4) Ig fusion protein to its CD80/CD86 receptor.
 Applications Tested/Suitable for anti-CD152 antibody   

Flow Cytometry (FC/FACS), ELISA (EIA), FX, Blocking

 Application Notes for anti-CD152 antibody    1:50 (or use 1.6ul of concentrated stock per 5 x 10^5-cell test)
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 Testing Data of anti-CD152 antibody    anti-CD152 antibody Testing Data image
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Product References and Citations for anti-CD152 antibody

   T. Lindsten, et al, (1993) J Immunol 151: 3489-3499. T.L. Walunas, et al, (1994) Immunity 1:405-413. N.J. Karandikar, et al, (1996) J Exp Med 184: 783-788. A. Kitani, et al, (2000) J Immunol 165: 691-702.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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