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Rat anti-Mouse CD16/32 (Fc gamma Receptor) Monoclonal Antibody

Scan QR to view Datasheet Catalog #    MBS673332 anti-CD16/32 (Fc gamma Receptor) antibody
Unit / Price
0.1 mg  /  $320 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

CD16/32 (Fc gamma Receptor), Monoclonal Antibody

 Also Known As   

Rat Anti-Mouse CD16/32-APC

 Product Synonym Names    Rat Anti-Mouse CD16/32 - Allophycocyanin; Rat Anti-Mouse CD16/32 (Fc gamma Receptor)
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Clonality    Monoclonal
 Isotype    Rat IgG2a
 Clone Number    93
 Host    Rat
 Species Reactivity    Mouse
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 Specificity    Murine CD16/32 (CD16/Fc gamma II and CD32/Fc gamma III receptors)
The lymphocyte Fc gamma receptors recognize the Fc portion of IgG, presented either as immune complexes or as free antibody. The different classes of receptors are distinct because of varying size, tissue distribution and affinity for IgGs. The Fc type II receptor is expressed on a wide variety of cells including B cells, hematopoietic cells, monocyte/macrophages, neutrophils, platelets, Langerhans cells, eosinophils, basophils, trophoblasts, and endothelial cells of the placenta.^1, 2 The Fc gamma type III receptors are higher affinity than the type II and are expressed on macrophages, NK cells and neutrophils. Both types of receptors can be expressed on the same cell and in varying ratios.^1 The receptors are constitutively expressed, although cytokines and lymphokines can modulate their expression.^4 Besides identifying Fc gamma R^+ cells, monoclonal antibodies to the Fc gamma II/III receptor have been used to block Fc receptor binding of IgG, Fcmediated signal transduction and effector functions, clearance of immune complexes and to attenuate infection by organisms dependent on Fc gamma R for parasitic invasion.^4-6 The monoclonal antibody 93 recognizes a conformational epitope formed by Fc gamma II and Fc gamma III receptors.
 Form/Format    Allophycocyanin (APC) Conjugate
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 Characterization    To insure lot-to-lot consistency, each batch of product is tested by flow cytometry for conformance with characteristics of a standard reference reagent.
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 Preparation and Storage    The purified (UNLB) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2-8 degree C.
The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The R-phycoerythrin (R-PE) conjugate is supplied as 0.1 mg in 1.0 mL or 0.2 mg in 2.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
The allophycocyanin (APC) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
The Spectral Red (SPRD), R-phycoerythrin-Cyanine 7 (R-PE-CY 7) and R-phycoerythrin-Cyanine 5.5 (R-PE-CY 5.5) conjugates are supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
The low endotoxin, azide-free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of PBS. Aliquot and store at or below -20 degree C.
Protect conjugated forms from light. Aliquot and freeze the low endotoxin, azide-free product at -20 degree C immediately upon receipt. Each reagent is stable for the period shown on the bottle label if stored as directed.
 Other Notes    Small volumes of anti-CD16/32 (Fc gamma Receptor) antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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 Applications Tested/Suitable for anti-CD16/32 (Fc gamma Receptor) antibody   

Flow Cytometry

 Application Notes for anti-CD16/32 (Fc gamma Receptor) antibody    Research Application(s): Blocking of Fc gamma receptors

Flow Cytometry Suggested Dilution: <= 0.2 ug/10^6 cells
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Product References and Citations for anti-CD16/32 (Fc gamma Receptor) antibody

   1. Unkeless, J. C., et al. 1995. Semin. Immunol. 7:37.
2. Warmerdam, P.A., et al. 1992. Immunobiol. 185:175.
3. Huizinga, T. W., et al. 1989. J. Immunol. 142:2365.
4. Ho, A. S-Y., et al. 1995. Mol. and Cell. Biol. 15:5043.
5. John Kearney, personal communication.
6. Kurlander, R.J., D. M. Ellison and J. Hall. 1984. J. Immunol. 133:855.
7. Araujo-Jorge, T., et al. 1993. Infection and Immunity 61:4925.
8. Titus, J. A., et al. 1984. J. Immunol. 133:556.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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