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anti-CD163 antibody :: Mouse CD163 Monoclonal Antibody

Scan QR to view Datasheet Catalog #    MBS216354
Unit / Price
0.1 mg  /  $290 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

CD163, Monoclonal Antibody

 Also Known As   

MOUSE ANTI RAT CD163

 Product Gene Name   

anti-CD163 antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 OMIM    605545
 3D Structure    ModBase 3D Structure for Q86VB7
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 Clonality    Monoclonal
 Isotype    IgG1
 Clone Number    ED2
 Host    Mouse
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 Form/Format    Purified
Purified IgG - liquid
 Concentration    IgG concentration 0.5 mg/ml (lot specific)
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 Perservative Stabilisers    0.09% Sodium Azide
Preparation
 Histology Positive Control Tissue    Liver
Buffer Solution
 Target Species    Rat
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 Preparation and Storage    Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.
 Other Notes    Small volumes of anti-CD163 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Product Description specifically for anti-CD163 antibody

   Mouse anti rat CD163, clone ED2 recognises the rat ED2 cell surface glycoprotein (Dijkstra et al. 1985). A 175 kDa molecule also known as rat CD163, a member of the group B scavenger receptor cysteine-rich (SRCR) family and an erythroblast adhesion receptor (Fabriek et al. 2007). Mouse anti rat CD163, clone ED2 was shown to detect approximately 50% of peritoneal macrophages, a subset of splenic macrophages, and most tissue macrophages. However, no staining was observed in monocytes or alveolar macrophages (Dijkstra et al. 1985, Beelen et al. 1987). In freshly isolated bone marrow, expression of CD163 was limited to mature macrophages only (Barbe et al. 1990). Clone ED2 may be used in immunohistology using antigen retrieval, and has also been described reacting with paraffin-embedded material following PLP fixation (Periodate-lysine-paraformaldehyde), see Whiteland et al.
 Applications Tested/Suitable for anti-CD163 antibody   

Immunohistology Frozen, Flow cytometry (FC/FACS), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistology Paraffin*, Western Blot (WB)

 Application Notes for anti-CD163 antibody    Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul
Immunohistology - Frozen: Minimum Dilution: 1/50; Maximum Dilution: 1/100
Flow Cytometry: Minimum Dilution: 1/10; Maximum Dilution: 1/100
Immunohistology - Paraffin: Application Note: This product requires protein digestion pre-treatment of paraffin sections e.g. trypsin or pronase
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 Testing Data of anti-CD163 antibody    Immunofluorescence staining of rat lymph node cryosection with Mouse anti Rat CD163 antibody , red an A and Mouse anti Rat CD8 , green in B. C is the merged image with nuclei counter-stained blue using DAPI. High power
anti-CD163 antibody Testing Data image
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 Testing Data of anti-CD163 antibody    Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD163 antibody followed by horseradish peroxidase conjugated Goat anti Mouse IgG1 (MBS235406) as a detection reagent. Medium power
anti-CD163 antibody Testing Data image
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 Testing Data of anti-CD163 antibody    Staining of acetone fixed, cryostat sectioned rat spleen with Mouse anti Rat CD163
anti-CD163 antibody Testing Data image
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 Testing Data of anti-CD163 antibody    Staining of rat peritoneal macrophages with Mouse anti Rat CD163: RPE (MBS216355)
anti-CD163 antibody Testing Data image
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 Testing Data of anti-CD163 antibody    Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD163 antibody followed by horseradish peroxidase conjugated Goat anti Mouse IgG1 (MBS235406) as a detection reagent. Medium power
anti-CD163 antibody Testing Data image
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 Testing Data of anti-CD163 antibody    Staining of rat peritoneal macrophages with Mouse anti Rat CD163: FITC (MBS216353)
anti-CD163 antibody Testing Data image
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 Testing Data of anti-CD163 antibody    Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD163 antibody followed by horseradish peroxidase conjugated Goat anti Mouse IgG1 (MBS235406) as a detection reagent. High power
anti-CD163 antibody Testing Data image
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 Testing Data of anti-CD163 antibody    Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD163 antibody followed by horseradish peroxidase conjugated Goat anti Mouse IgG1 (MBS235406) as a detection reagent. Low power
anti-CD163 antibody Testing Data image
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 Testing Data of anti-CD163 antibody    Immunofluorescence staining of rat lymph node cryosection with Mouse anti Rat CD163 antibody , red an A and Mouse anti Rat CD8 , green in B. C is the merged image with nuclei counter-stained blue using DAPI. Low power
anti-CD163 antibody Testing Data image
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NCBI/Uniprot data below describe general gene information for CD163. It may not necessarily be applicable to this product.
 NCBI GI #    5107945
 NCBI GeneID    9332
 NCBI Accession #    CAB45233.1 [Other Products]
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 UniProt Primary Accession #    Q86VB7 [Other Products]
 UniProt Secondary Accession #    Q07898; Q07899; Q07900; Q07901; Q2VLH7; C9JIG2 [Other Products]
 UniProt Related Accession #    Q86VB7 [Other Products]
 Molecular Weight    124,958 Da
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 NCBI Official Full Name    CD163
 NCBI Official Synonym Full Names    CD163 molecule
 NCBI Official Symbol    CD163 [Similar Products]
 NCBI Official Synonym Symbols   
M130; MM130
[Similar Products]
 NCBI Protein Information    scavenger receptor cysteine-rich type 1 protein M130; hemoglobin scavenger receptor; macrophage-associated antigen
 UniProt Protein Name    Scavenger receptor cysteine-rich type 1 protein M130
 UniProt Synonym Protein Names   
Hemoglobin scavenger receptor; CD_antigen: CD163Soluble CD163; sCD163
 Protein Family    Scavenger receptor cysteine-rich type 1 protein
 UniProt Gene Name    CD163 [Similar Products]
 UniProt Synonym Gene Names    M130; sCD163 [Similar Products]
 UniProt Entry Name    C163A_HUMAN
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 NCBI Summary for CD163    The protein encoded by this gene is a member of the scavenger receptor cysteine-rich (SRCR) superfamily, and is exclusively expressed in monocytes and macrophages. It functions as an acute phase-regulated receptor involved in the clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages, and may thereby protect tissues from free hemoglobin-mediated oxidative damage. This protein may also function as an innate immune sensor for bacteria and inducer of local inflammation. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Aug 2011]
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 UniProt Comments for CD163    CD163: Acute phase-regulated receptor involved in clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages and may thereby protect tissues from free hemoglobin-mediated oxidative damage. May play a role in the uptake and recycling of iron, via endocytosis of hemoglobin/haptoglobin and subsequent breakdown of heme. Binds hemoglobin/haptoglobin complexes in a calcium-dependent and pH-dependent manner. Exhibits a higher affinity for complexes of hemoglobin and multimeric haptoglobin of HP*1F phenotype than for complexes of hemoglobin and dimeric haptoglobin of HP*1S phenotype. Induces a cascade of intracellular signals that involves tyrosine kinase-dependent calcium mobilization, inositol triphosphate production and secretion of IL6 and CSF1. Isoform 3 exhibits the higher capacity for ligand endocytosis and the more pronounced surface expression when expressed in cells. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 12p13.3

Cellular Component: integral to plasma membrane; plasma membrane; extracellular region

Molecular Function: protein binding; scavenger receptor activity

Biological Process: receptor-mediated endocytosis; acute-phase response
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 Research Articles on CD163    1. propose the serum sCD163 value 1.8 mg/L as a cutoff concentration for survival analysis in patients with multiple myeloma, which should be validated in future studies
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-CD163 antibodyPathways associated with anti-CD163 antibody
 Reference Product  PubMed Publications
 CD68 antibody  >402 publications with CD163 and CD68
 IL10 antibody  >99 publications with CD163 and IL10
 HMOX1 antibody  >46 publications with CD163 and HMOX1
 CSF1R antibody  >31 publications with CD163 and CSF1R
 IL6 antibody  >15 publications with CD163 and IL6
 C1QB antibody  >1 publications with CD163 and C1QB
 VSIG4 antibody  >1 publications with CD163 and VSIG4
 Products by Pathway  Pathway Diagram
 Binding And Uptake Of Ligands By Scavenger Receptors Pathway antibodies  Binding And Uptake Of Ligands By Scavenger Receptors Pathway Diagram
 Scavenging Of Heme From Plasma Pathway antibodies  Scavenging Of Heme From Plasma Pathway Diagram
Diseases associated with anti-CD163 antibodyOrgans/Tissues associated with anti-CD163 antibody
 Disease Name  Pubmed Publications
 Inflammation Antibodies  >399 publications with CD163 and Inflammation
 Necrosis Antibodies  >180 publications with CD163 and Necrosis
 Cardiovascular Diseases Antibodies  >114 publications with CD163 and Cardiovascular Diseases
 Nervous System Diseases Antibodies  >108 publications with CD163 and Nervous System Diseases
 Brain Diseases Antibodies  >67 publications with CD163 and Brain Diseases
 Disease Models, Animal Antibodies  >66 publications with CD163 and Disease Models, Animal
 Fibrosis Antibodies  >60 publications with CD163 and Fibrosis
 Lung Diseases Antibodies  >55 publications with CD163 and Lung Diseases
 Adenocarcinoma Antibodies  >43 publications with CD163 and Adenocarcinoma
 Heart Diseases Antibodies  >38 publications with CD163 and Heart Diseases
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >653 publications with CD163 and Blood
 Vascular Antibodies  >181 publications with CD163 and Vascular
 Skin Antibodies  >121 publications with CD163 and Skin
 Bone Antibodies  >112 publications with CD163 and Bone
 Liver Antibodies  >112 publications with CD163 and Liver
 Lung Antibodies  >80 publications with CD163 and Lung
 Brain Antibodies  >75 publications with CD163 and Brain
 Kidney Antibodies  >48 publications with CD163 and Kidney
 Connective Tissue Antibodies  >48 publications with CD163 and Connective Tissue
 Heart Antibodies  >47 publications with CD163 and Heart
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