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anti-CD20 antibody :: Mouse CD20 Monoclonal Antibody

Scan QR to view Datasheet Catalog #    MBS370021
Unit / Price
5 Control Slides  /  $130 +1 FREE 8GB USB
3 ml (Prediluted)  /  $165 +1 FREE 8GB USB
0.1 mL (Concentrate)  /  $170 +1 FREE 8GB USB
7 ml (Prediluted)  /  $185 +1 FREE 8GB USB
0.5 mL (Concentrate)  /  $240 +1 FREE 8GB USB
15 ml (Prediluted)  /  $285 +1 FREE 8GB USB
1 mL (Concentrate)  /  $290 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

CD20, Monoclonal Antibody

★Popular Item★
 Also Known As   

CD20

 Product Gene Name   

anti-CD20 antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Chromosome Location    Chromosome: 21; NC_006603.2 (53903583..53913185)
 3D Structure    ModBase 3D Structure for Q3C2E2
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 Clonality    Monoclonal
 Isotype    IgG2a/K
 Clone Number    L26
 Host    Mouse
 Species Reactivity    Human, Canine, Feline, Mouse
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 Form/Format    CD20 is a mouse monoclonal antibody derived from cell culture supernatant that is concentrated, dialyzed, filter sterilized and diluted in buffer pH 7.5, containing BSA and sodium azide as a preservative.
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 Immunogen    Human Tonsil B cells
 Control    Tonsil, Lymph Node
 Localization    Membranous
 Reactivity Note    Paraffin, Frozen
 Specimen Preparation    Paraffin sections: The antibody can be used on formalin-fixed paraffin-embedded (FFPE) tissue sections. Ensure tissue undergoes appropriate fixation to ensure best results. Pre-treatment of tissues with heat-induced epitope retrieval (HIER) is recommended using ImmunoDNA Retriever with Citrate, ImmunoDNA Retriever with EDTA or ImmunoDNA Digestor. Tissue should remain hydrated via use of Immuno/DNA Washer solutions.
Frozen sections and cell preparations: The antibody can be used for labeling acetone-fixed frozen sections and acetone-fixed cell preparations.
 Staining Procedure    1. Cut and mount 3-5 micron formalin-fixed paraffin-embedded tissues on positive charged slides such as Hydrophilic Plus Slides.
2. Air dry for 2 hours at 58° C.
3. Deparaffinize, dehydrate and rehydrate tissues.
4. Subject tissues to heat epitope retrieval using a suitable retrieval solution such as ImmunoDNA Retriever with Citrate or EDTA
5. Any of three heating methods may be used:
a. TintoRetriever Pressure Cooker or Equivalent
Place tissues/slides in a staining dish or coplin jar containing the ImmunoDNA Retriever with Citrate or EDTA, and place in the pressure cooker. Add 1-2 inches of distilled water to the pressure cooker and turn heat to high. Incubate for 15 minutes. Open and immediately transfer slides to room temperature.
b. TintoRetriever PT Module or Water Bath Method
Place tissues/slides in a pre-warmed staining dish or coplin jar containing the ImmunoDNA Retriever with Citrate or EDTA at 95°-99° C. Incubate for 30-60 minutes.
c. Conventional Steamer Method
Place tissues/slides in a pre-warmed staining dish or coplin jar containing the ImmunoDNA Retriever with Citrate or EDTA in a Steamer, cover and steam for 30-60 minutes.
6. After heat treatment, transfer slides in ImmunoDNA Retriever with Citrate or EDTA to room temperature and let stand for 15-20 minutes.
7. For manual staining, perform antibody incubation at ambient temperature. For automated staining methods, perform antibody incubation according to instrument manufacturer’s instructions.
8. Wash slides with IHC wash buffer or DI water.
9. Continue IHC staining protocol.
 Product Limitations    Due to inherent variability present in immunohistochemical procedures (including fixation time of tissues, dilution factor of antibody, retrieval method utilized and incubation time), optimal performance should be established through the use of positive and negative controls. Results should be interpreted by a medical professional.
 Note    This product contains sodium azide (NaN3), a toxic chemical which may react with plumbing to form highly explosive build-ups of metal azides. Upon disposal, flush with large volumes of water to prevent sodium azide build-up.
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 Preparation and Storage    Store at 2 to 8 degree C. Do not use after expiration date listed on package label. Temperature fluctuations should be avoided. Store appropriately when not in use, and avoid prolonged exposure to room temperature conditions.
 Other Notes    Small volumes of anti-CD20 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for anti-CD20 antibody

   CD20 is a transmembrane, non-glycosylated protein expressed on B-cell precursors and mature B-cells, but lost following differentiation into plasma cells. This antibody does not cross-react with non-hematopoietic neoplasms. CD20 (B-cell Pan) reacts with a membrane antigen present in B-cells. This antibody strongly recognizes Reed-Sternberg cells predominant in Hodgkin's disease. Since no staining of histiocytes or plasma cells has been observed and CD20 has not been detected in T-cell malignancies, it is a very strong marker of B-cell Lymphomas. B-cell Panmarker recognizes a formalin-resistant intracytoplasmic antigen.
 Application Notes for anti-CD20 antibody    Intended Use: This antibody is intended for use in Immunohistochemical applications on formalin fixed paraffin-embedded tissues (FFPE), frozen tissue sections and cell preparations. Interpretations of results should be performed by a qualified medical professional.
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 Dilution Information of anti-CD20 antibody    anti-CD20 antibody Dilution Information image
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 Recommended IHC Protocol of anti-CD20 antibody    anti-CD20 antibody Recommended IHC Protocol image
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 Performance Characteristics of anti-CD20 antibody    anti-CD20 antibody Performance Characteristics image
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NCBI/Uniprot data below describe general gene information for CD20. It may not necessarily be applicable to this product.
 NCBI GI #    78183404
 NCBI GeneID    485430
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 UniProt Primary Accession #    Q3C2E2 [Other Products]
 UniProt Related Accession #    Q3C2E2 [Other Products]
 Molecular Weight    33,056 Da
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 NCBI Official Full Name    CD20
 NCBI Official Symbol    MS4A1 [Similar Products]
 NCBI Official Synonym Symbols   
CD20
[Similar Products]
 NCBI Protein Information    B-lymphocyte antigen CD20; membrane-spanning 4-domains subfamily A member 1
 UniProt Protein Name    B-lymphocyte antigen CD20
 UniProt Synonym Protein Names   
Membrane-spanning 4-domains subfamily A member 1
 Protein Family    CD209 antigen
 UniProt Gene Name    MS4A1 [Similar Products]
 UniProt Synonym Gene Names    CD20 [Similar Products]
 UniProt Entry Name    CD20_CANFA
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 UniProt Comments for CD20    Function: This protein may be involved in the regulation of B-cell activation and proliferation

Subcellular location: Membrane; Multi-pass membrane protein

Tissue specificity: Expressed in PBMCs and lymph node from healthy dogs, in B-cells of canine lymphoma, but not in T-cell lymphoma cells and non-T and non-B-cell lymphoma cells. Ref.1

Post-translational modification: Phosphorylated. Might be functionally regulated by protein kinase(s)

Sequence similarities: Belongs to the MS4A family.
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 Research Articles on CD20    1. This study examined the immunoreactivity of a monoclonal antibody to MUM1/IRF4 and compared it with expression of CD79a and CD20 in 109 plasmacytomas in 107 dogs.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with anti-CD20 antibodyDiseases associated with anti-CD20 antibody
 Products by Pathway  Pathway Diagram
 Hematopoietic Cell Lineage Pathway antibodies  Hematopoietic Cell Lineage Pathway Diagram
 Hematopoietic Cell Lineage Pathway antibodies  Hematopoietic Cell Lineage Pathway Diagram
 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >2700 publications with CD20 and Neoplasms
 Lymphoma Antibodies  >2143 publications with CD20 and Lymphoma
 Leukemia Antibodies  >833 publications with CD20 and Leukemia
 Autoimmune Diseases Antibodies  >560 publications with CD20 and Autoimmune Diseases
 Lymphoma, T-Cell Antibodies  >431 publications with CD20 and Lymphoma, T-Cell
 Skin Diseases Antibodies  >326 publications with CD20 and Skin Diseases
 Cardiovascular Diseases Antibodies  >303 publications with CD20 and Cardiovascular Diseases
 Vascular Diseases Antibodies  >293 publications with CD20 and Vascular Diseases
 Inflammation Antibodies  >218 publications with CD20 and Inflammation
 Arthritis, Rheumatoid Antibodies  >214 publications with CD20 and Arthritis, Rheumatoid
Organs/Tissues associated with anti-CD20 antibody
 Organ/Tissue Name  Pubmed Publications
 Artery Antibodies  >36 publications with CD20 and Artery
 Lymphoreticular Antibodies  >4 publications with CD20 and Lymphoreticular
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