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anti-CD205 antibody :: Rat CD205 Monoclonal Antibody

Scan QR to view Datasheet Catalog #    MBS216696
Unit / Price
0.025 mg  /  $175 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

CD205, Monoclonal Antibody

★Popular Item★
 Also Known As   

RAT ANTI MOUSE CD205:FITC

 Product Gene Name   

anti-CD205 antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 3D Structure    ModBase 3D Structure for Q60767
 Clonality    Monoclonal
 Isotype    IgG2a
 Clone Number    NLDC-145
 Host    Rat
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 Form/Format    FITC
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
 Concentration    IgG concentration 0.1 mg/ml (lot specific)
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 Perservative Stabilisers    0.09% Sodium Azide
1% Bovine Serum Albumin
Preparation
 Immunogen    Mouse lymph node stroma
Buffer Solution
 Target Species    Mouse
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 Preparation and Storage    Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.
 Other Notes    Small volumes of anti-CD205 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for anti-CD205 antibody

   Rat anti Mouse CD205, clone NLDC-145 recognizes DEC-205, a 205kDa integral membrane glycoprotein expressed by mouse dendritic cells and thymic epithelial cells. It is absent in bone marrow cells, all blood cells and freshly isolated macrophages from the peritoneal cavity. A subpopulation of the latter becomes positive in mice previously stimulated with thioglycollate. It is also absent on follicular dendritic cells of B cell follicles. The antigen is found on la positive interdigitating cells in T cell areas of all secondary lymphoid organs. It is also present on veiled cells. In non-lymphoid organs, the antigen is only found on Langerhans cells of the skin but cross reactions with la positive thymic epithelial cells and intestinal villus epithelial cells are also observed. DEC-205 is expressed at the cell surface of unfixed cells but can also be found in the cytoplasm of fixed cells.
 Applications Tested/Suitable for anti-CD205 antibody   

Flow cytometry (FC/FACS)

 Application Notes for anti-CD205 antibody    Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul. The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR
Flow Cytometry: Minimum Dilution: Neat; Maximum Dilution: 1/10
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 Testing Data of anti-CD205 antibody    Staining of mouse thymus cells with Rat anti Mouse CD205:FITC (MBS216695)
anti-CD205 antibody Testing Data image
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 Testing Data of anti-CD205 antibody    Immunoperoxidase staining of cryosectioned mouse lymph node with Rat anti Mouse CD205 (MBS216692)
anti-CD205 antibody Testing Data image
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 Testing Data of anti-CD205 antibody    Immunoperoxidase staining of mouse dendritic cells with Rat anti Mouse CD205 (MBS216692)
anti-CD205 antibody Testing Data image
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 Testing Data of anti-CD205 antibody    Immunofluorescent staining of Langerhans cells in mouse skin with Rat anti Mouse CD205 (MBS216692)
anti-CD205 antibody Testing Data image
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 Testing Data of anti-CD205 antibody    Published customer image: AIRE expression in the unreverted R/-; CreERt2 thymic rudiment. 3 -4 months old R/-; CreERt2 mice were injected with 4OHT at the doses shown (A, carrier-only, B, 0.25 mg 4OHT, C, 2 mg 4OHT). Images show immunohistochemical analysis of thymi or thymic rudiments from 4OHT mice 7 weeks post-injection. Staining is shown for AIRE, counterstained for cytokeratin 8 (K8) or CD205 as shown. DAPI reveals nuclei (blue). (A) Top and bottom panels show representative images of AIRE- regions (which comprised the majority of sections), and a rare AIRE+ cell, respectively. (B) Top and middle panels show representative images of AIRE- regions and AIRE+ cells, respectively. Bottom panel shows higher power image of middle panel. Carrier-only and 0.25 mg 4OHT injected mice, n = 2; reverted mice (i.e. injected with =0.5 mg 4OHT), n>3 (shown for 2 mg injected). Scale bars 100 um except where shown.From: Jin X, Nowell CS, Ulyanchenko S, Stenhouse FH, Blackburn CC (2014) Long-Term Persistence of Functional Thymic Epithelial Progenitor Cells In Vivo under Conditions of Low FOXN1 Expression. PLoS ONE 9(12): e114842.
anti-CD205 antibody Testing Data image
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NCBI/Uniprot data below describe general gene information for CD205. It may not necessarily be applicable to this product.
 NCBI GI #    148747179
 NCBI GeneID    17076
 NCBI Accession #    NP_038853.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_013825.3 [Other Products]
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 UniProt Primary Accession #    Q60767 [Other Products]
 UniProt Secondary Accession #    Q8C7T3; Q91XL8; Q9QUZ6; B2RWW5 [Other Products]
 UniProt Related Accession #    Q60767 [Other Products]
 Molecular Weight    197,352 Da
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 NCBI Official Full Name    lymphocyte antigen 75
 NCBI Official Synonym Full Names    lymphocyte antigen 75
 NCBI Official Symbol    Ly75 [Similar Products]
 NCBI Official Synonym Symbols   
CD205; DEC205; DEC-205
[Similar Products]
 NCBI Protein Information    lymphocyte antigen 75; ly-75
 UniProt Protein Name    Lymphocyte antigen 75
 UniProt Synonym Protein Names   
DEC-205; CD_antigen: CD205
 UniProt Gene Name    Ly75 [Similar Products]
 UniProt Synonym Gene Names    Cd205; Ly-75 [Similar Products]
 UniProt Entry Name    LY75_MOUSE
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 UniProt Comments for CD205    LY75: Acts as an endocytic receptor to direct captured antigens from the extracellular space to a specialized antigen- processing compartment. Causes reduced proliferation of B-lymphocytes. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Cellular Component: membrane; integral to membrane; external side of plasma membrane

Molecular Function: carbohydrate binding

Biological Process: endocytosis
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 Research Articles on CD205    1. The DEC-205-mediated antigen targeting to steady-state dendritic cells induces deletion of diabetogenic CD8 T cells independently of PD-1 and PD-L1.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Diseases associated with anti-CD205 antibodyOrgans/Tissues associated with anti-CD205 antibody
 Disease Name  Pubmed Publications
 Cardiomegaly Antibodies  >1 publications with CD205 and Cardiomegaly
 Ovarian Neoplasms Antibodies  >1 publications with CD205 and Ovarian Neoplasms
 Ovarian Diseases Antibodies  >1 publications with CD205 and Ovarian Diseases
 Heart Diseases Antibodies  >1 publications with CD205 and Heart Diseases
 Neoplasm Metastasis Antibodies  >1 publications with CD205 and Neoplasm Metastasis
 Organ/Tissue Name  Pubmed Publications
 Heart Antibodies  >1 publications with CD205 and Heart
 Mammary Gland Antibodies  >1 publications with CD205 and Mammary Gland
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