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anti-CD206 antibody :: Rat CD206 Monoclonal Antibody

Scan QR to view Datasheet Catalog #    MBS215663
Unit / Price
0.05 mg  /  $235 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

CD206, Monoclonal Antibody

 Also Known As   

RAT ANTI MOUSE CD206:FITC

 Product Gene Name   

anti-CD206 antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Clonality    Monoclonal
 Isotype    IgG2a
 Clone Number    MR5D3
 Host    Rat
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 Form/Format    FITC
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
 Concentration    IgG concentration 0.1 mg/ml (lot specific)
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 Perservative Stabilisers    0.09% Sodium Azide
1% Bovine Serum Albumin
Preparation
 Buffer Solution    Phosphate buffered saline
 Target Species    Mouse
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 Preparation and Storage    Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.
 Other Notes    Small volumes of anti-CD206 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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 Applications Tested/Suitable for anti-CD206 antibody   

Flow cytometry (FC/FACS)*

 Application Notes for anti-CD206 antibody    Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul. The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR.
Flow Cytometry: Minimum Dilution: neat; Application Note: CD206 is expressed weakly at the cell surface. Staining may be increased following membrane permeabilisation. MyBioSource recommends the use of Leucoperm for this purpose.
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 Testing Data of anti-CD206 antibody    Staining of mouse peritoneal macrophages with Rat anti Mouse CD206: Alexa Fluor 647 (MBS215657A647)
anti-CD206 antibody Testing Data image
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 Testing Data of anti-CD206 antibody    Immunoperoxidase staining of a mouse lymph node cryosection with Rat anti Mouse CD206 antibody, clone MR5D3 (MBS215657) followed by horseradish peroxidase conjugated Goat anti Rat IgG antibody (MBS235195). Medium power
anti-CD206 antibody Testing Data image
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 Testing Data of anti-CD206 antibody    Immunofluorescence staining of a mouse lymph node cryosection with Rat anti Mouse CD206 antibody, clone MR5D3 (MBS215657), green in A and Rat anti Mouse CD8 antibody, clone YTS105.18 , red in B. C is the merged image with nuclei counterstained in blue using DAPI. High power
anti-CD206 antibody Testing Data image
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 Testing Data of anti-CD206 antibody    Staining of mouse peritoneal macrophages with Rat anti Mouse CD206: Alexa Fluor 488 (MBS215657A488)
anti-CD206 antibody Testing Data image
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 Testing Data of anti-CD206 antibody    Staining of J774 cell line with Rat anti Mouse CD206 following permeabilisation with Leucoperm
anti-CD206 antibody Testing Data image
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 Testing Data of anti-CD206 antibody    Immunoperoxidase staining of a mouse lymph node cryosection with Rat anti Mouse CD206 antibody, clone MR5D3 (MBS215657) followed by horseradish peroxidase conjugated Goat anti Rat IgG antibody (MBS235195). Low power
anti-CD206 antibody Testing Data image
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 Testing Data of anti-CD206 antibody    Immunoperoxidase staining of a mouse lymph node cryosection with Rat anti Mouse CD206 antibody, clone MR5D3 (MBS215657) followed by horseradish peroxidase conjugated Goat anti Rat IgG antibody (MBS235195). High power
anti-CD206 antibody Testing Data image
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 Testing Data of anti-CD206 antibody    Immunofluorescence staining of a mouse lymph node cryosection with Rat anti Mouse CD206 antibody, clone MR5D3 (MBS215657), green in A and Rat anti Mouse CD8 antibody, clone YTS105.18 , red in B. C is the merged image with nuclei counterstained in blue using DAPI. Low power
anti-CD206 antibody Testing Data image
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 Testing Data of anti-CD206 antibody    Published customer image: Western blot analysis to determine type of activation of adult primary microglial cells produced from wild-type and IL-1 KO mice stimulated with IL-4, IL-13 or IL-4/IL-13 with or without IL-1beta. (A) Representative western blotting data of primary microglial cells produced from wild-type (wild) and IL-1 KO mice and exposed for 24 hours to IL-4, IL-13 or IL-4 plus IL-13 (IL-4/IL-13) with or without IL-1beta. Each lane expected to CD206 blotting were applied 7 ug of reduced samples. Non-reduced samples (5 ug) were applied to detect CD206. Densitometric analysis of COX2 (B), Ym1 (C), Arg-1 (D) and CD206 (E) (n = 3 each group). (B) COX2 levels are increased by exposure of cells to IL-1beta and are not influenced by IL-4 or IL-13 alone. The COX2 level was slightly enhanced by IL-1beta and IL-4 co-treatment. (C) Ym1 levels are increased by exposure of cells to IL-4 and IL-4/Il-13 and are synergistically increased by co-treatment with IL-1beta. However, only a low level of Ym1 is seen upon exposure of cells to IL-13, and is significantly less than that seen in response to exposure of cells to IL-4. (D) Arg-1 shows similar levels in response to exposure to IL-4 and IL-4/IL-13; these are synergistically increased by co-treatment with IL-1beta. However, low levels of Arg-1 are seen for exposure of cells to IL-13. (G) CD206 was detected in response to exposure of cells to both IL-4 and IL-4/IL-13 with or without IL-1beta; however, CD206 levels in IL-13-exposed samples were lower than those seen with the other treatments. Data are expressed as mean +/- SD (n = 3). *: P < 0.05, **: P < 0.01, ***: P < 0.001 compared with the IL-4-treated group without IL-1beta for each genotype (one-way ANOVA followed by Dunnett post-hoc test). ANOVA, analysis of variance; arg-1, arginase 1; COX 2, cyclooxygenase 2.From: Sato A, Ohtaki H, Tsumuraya T, Song D, Ohara K, Asano M, Iwakura Y, Atsumi T, Shioda S. Interleukin-1 participates in the classical and alternative activation of microglia/macrophages after spinal cord injury. J Neuroinflammation. 2012 Apr 7;9:65.
anti-CD206 antibody Testing Data image
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 Testing Data of anti-CD206 antibody    Published customer image: Increased accumulation of repair-associated macrophages surrounding collaterals in ischemic hind limbs is PAR2-dependent. (A) Stainings of CD206-positive macrophages (green) and SMA-positive vessels (red) in non-ischemic (control) and ischemic (ligated) hind limbs of WT, PAR1-/- and PAR2-/- mice are shown. Nuclei were visualized with DAPI (blue). Arrows indicate single macrophages in the non-ischemic adductor. Quantification of the average number of repair-associated macrophages per vessel is indicated on the right. (B) Correlation between the number of CD206-positive macrophages in the ischemic tissues and the expression of CD11b and (C) CD115 on monocytes. ** p
anti-CD206 antibody Testing Data image
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NCBI/Uniprot data below describe general gene information for CD206. It may not necessarily be applicable to this product.
 NCBI GI #    224967062
 NCBI GeneID    17533
 NCBI Accession #    NP_032651.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_008625.2 [Other Products]
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 UniProt Secondary Accession #    Q8C502 [Other Products]
 UniProt Related Accession #    Q61830 [Other Products]
 Molecular Weight    164,981 Da
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 NCBI Official Full Name    macrophage mannose receptor 1
 NCBI Official Synonym Full Names    mannose receptor, C type 1
 NCBI Official Symbol    Mrc1 [Similar Products]
 NCBI Official Synonym Symbols   
MR; CD206; AW259686
[Similar Products]
 NCBI Protein Information    macrophage mannose receptor 1; MMR
 UniProt Protein Name    Macrophage mannose receptor 1
 UniProt Synonym Protein Names   
CD_antigen: CD206
 UniProt Gene Name    Mrc1 [Similar Products]
 UniProt Synonym Gene Names    MMR [Similar Products]
 UniProt Entry Name    MRC1_MOUSE
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 UniProt Comments for CD206    MRC1: Mediates the endocytosis of glycoproteins by macrophages. Binds both sulfated and non-sulfated polysaccharide chains. Acts as phagocytic receptor for bacteria, fungi and other pathogens. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Receptor, misc.; Motility/polarity/chemotaxis

Cellular Component: cell surface; membrane; plasma membrane; integral to membrane; endosome membrane; endosome

Molecular Function: mannose binding; transmembrane receptor activity; receptor activity; carbohydrate binding

Biological Process: receptor-mediated endocytosis; endocytosis; signal transduction
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 Research Articles on CD206    1. These findings suggest that MR plays a major role in Enterococcus faecalis strain EC-12 phagocytosis by the antigen-presenting cells.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with anti-CD206 antibodyDiseases associated with anti-CD206 antibody
 Products by Pathway  Pathway Diagram
 Phagosome Pathway antibodies  Phagosome Pathway Diagram
 Phagosome Pathway antibodies  Phagosome Pathway Diagram
 Tuberculosis Pathway antibodies  Tuberculosis Pathway Diagram
 Tuberculosis Pathway antibodies  Tuberculosis Pathway Diagram
 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >21 publications with CD206 and Nervous System Diseases
 Inflammation Antibodies  >17 publications with CD206 and Inflammation
 Necrosis Antibodies  >8 publications with CD206 and Necrosis
 Lung Diseases Antibodies  >7 publications with CD206 and Lung Diseases
 Disease Models, Animal Antibodies  >3 publications with CD206 and Disease Models, Animal
 Weight Loss Antibodies  >3 publications with CD206 and Weight Loss
 Fibrosis Antibodies  >2 publications with CD206 and Fibrosis
 Colitis Antibodies  >2 publications with CD206 and Colitis
 Liver Diseases Antibodies  >1 publications with CD206 and Liver Diseases
 Breast Neoplasms Antibodies  >1 publications with CD206 and Breast Neoplasms
Organs/Tissues associated with anti-CD206 antibody
 Organ/Tissue Name  Pubmed Publications
 Bone Antibodies  >5 publications with CD206 and Bone
 Heart Antibodies  >4 publications with CD206 and Heart
 Lung Antibodies  >4 publications with CD206 and Lung
 Liver Antibodies  >4 publications with CD206 and Liver
 Spleen Antibodies  >4 publications with CD206 and Spleen
 Embryonic Tissue Antibodies  >2 publications with CD206 and Embryonic Tissue
 Pancreas Antibodies  >1 publications with CD206 and Pancreas
 Uterus Antibodies  >1 publications with CD206 and Uterus
 Mammary Gland Antibodies  >1 publications with CD206 and Mammary Gland
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