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anti-CD235 antibody :: Mouse anti-Human CD235 Monoclonal Antibody

Scan QR to view Datasheet Catalog #    MBS666642
Testing Data
Unit / Price
0.1 mg  /  $270 +1 FREE 8GB USB
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 Product Name   

CD235, Monoclonal Antibody

 Also Known As   

anti-human CD235(Glycophorin A)-Purified Preservative Free

 Product Synonym Names    Monoclonal anti- human CD235a (Glycophorin A)
 Product Gene Name   

anti-CD235 antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Clonality    Monoclonal
 Isotype    IgMk
 Clone Number    A63-B/C2
 Host    Mouse
 Species Reactivity    Human
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 Purity/Purification    Purified Preservative Free
 Form/Format    50 mM Sodium Phosphate pH 7.5, 500 mM Potassium Chloride, 150mM NaCl
 Concentration    1.0 mg/ml (lot specific)
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 Performance    Ten x 106 human red blood cells were washed and preincubated 5 minutes with 20 ul of 250 ug/ml human IgG (to block non specific binding) after which they were incubated 45 minutes on ice with 80 ul of anti-CD235a (Glycophorin A) antibody at 10 ug/ml. Cells were washed twice and incubated with 2 degree reagent Goat anti-Mouse IgG/FITC; after which they were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 0.9 log10 fluorescent units when compared to a Mouse IgM negative control at a similar concentration.
 Production    Antibody from (low FBS containing) tissue culture supernatant was purified to 95% mouse immunoglobulin by SDS-PAGE (<1% bovine immunoglobulin) using size exclusion chromatography. Product was 0.2 um filtered and vialed under aseptic conditions.
 Buffer    50 mM Sodium Phosphate pH 7.5, 500 mM Potassium Chloride, 150mM NaCl.
 Immunogen    Neuraminidase treated human erythrocytes
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 Preparation and Storage    Store at 2 - 5 degree C. Freeze/Thawing is not recommended. Open under aseptic conditions.
Product should retain activity for at least 12 months after shipping date when stored as recommended.
 Other Notes    Small volumes of anti-CD235 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for anti-CD235 antibody

   Information: Human CD235a (glycophorin A) is a sialoglycoprotein expressed early on the cell surface during erythropoiesis as pluripotent stem cells are recruited to differentiate into erythrocytes (1). CD235a (glycophorin A) is a useful marker to study the developmental biology of hematopoietic progenitor cells (2,3). Antibody A63-B/C2 reacts with an extracellular (peptide) domain of CD235a (glycophorin A).
 Applications Tested/Suitable for anti-CD235 antibody   

Flow Cytometry (FC/FACS)

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 Testing Data of anti-CD235 antibody    anti-CD235 antibody Testing Data image
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Product References and Citations for anti-CD235 antibody

   J.G. Mark, et al, (1999) Blood 93: 4425-4435. 2. N. Debili, et al, (1996) Blood 88: 1284-1296. 3. B.M. Frey, et al, (1998) Blood 91: 2781-2792.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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