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anti-CD25 antibody :: Mouse CD25/ IL2RA Monoclonal Antibody

Scan QR to view Datasheet Catalog #    MBS4380450
Unit / Price
0.02 mg (With BSA & Azide at 0.2mg/ml)  /  $190 +1 FREE 8GB USB
0.1 mg (With BSA & Azide at 0.2mg/ml)  /  $340 +1 FREE 8GB USB
0.1 mg (Without BSA & Azide at 1mg/ml)  /  $340 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

CD25/ IL2RA, Monoclonal Antibody

★Popular Item★
 Also Known As   

CD25/ IL2RA (Activated Lymphocyte Marker)

 Product Synonym Names    IL2 Receptor alpha; IL2R alpha chain; IL2R; IL2R subunit alpha IL2RA; Interleukin-2 receptor subunit alpha; Ly43; p55; p55 chain; T Cell Growth Factor Receptor; TAC antigen; TCGFR
 Product Gene Name   

anti-CD25 antibody

[Similar Products]
 Product Synonym Gene Name    IL2RA [Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 3D Structure    ModBase 3D Structure for P01589
 Clonality    Monoclonal
 Isotype    IgG2b
 Clone Number    IL2RA/2395
 Host    Mouse
 Species Reactivity    Human. Others not tested.
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 Specificity    Recognizes a protein of 55kDa, identified as CD25.It is expressed on activated T- and B-cells and activated monocytes/macrophages.With respect to lymphomas, CD25 is present on malignant cells of Hodgkins disease, HTLV-1+ adult T-cell leukemia, cutaneous T-cell lymphoma, and hair cell leukemia. Increased levels of soluble CD25 are observed in the leukemias/lymphomas and inflammatory/ autoimmune diseases. CD25 molecule alone appears to function as a low affinity receptor and associates with CD122 (IL-2R  chain, p75) and CD132 (common chain) to form the high affinity IL-2 receptor complex.CD25 antibodies detect three epitope regions, A, B and C. This MAb recognizes the epitope B, which is located at residue 3-104 of CD25 and doe not block IL-2 binding to CD25.
 Form/Format    200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
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 Immunogen    Recombinantfragment of human IL2RA protein (around aa 42-183)
 Cellular Localization    Cell Surface and Cytoplasmic
 Hu-chromosome Location    10p15-p14
 Positive Control    PHA-stimulated human lymphocytes. Human lymph nodeor tonsil
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 Other Notes    Small volumes of anti-CD25 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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 Applications Tested/Suitable for anti-CD25 antibody   

Immunohistochemistry (IHC) Formalin

 Application Notes for anti-CD25 antibody    IHC-F: 2-4ug/ml for 30 minutes at RT; Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
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 Immunohistochemistry (IHC) of anti-CD25 antibody    Formalin-fixed, paraffin-embeddedhuman Tonsil stained with CD25Mouse Monoclonal Antibody (IL2RA/2395).
anti-CD25 antibody Immunohistochemistry (IHC) (IHC) image
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 SDS-Page of anti-CD25 antibody    SDS-PAGE Analysis Purified CD25Mouse Monoclonal Antibody (IL2RA/2395).Confirmation of Integrity and Purity of Antibody
anti-CD25 antibody SDS-Page image
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 Testing Data of anti-CD25 antibody    Analysis of Protein Array containing >19, 000 full-length human proteins using CD25 Mouse Monoclonal Antibody (IL2RA/2395)Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
anti-CD25 antibody Testing Data image
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NCBI/Uniprot data below describe general gene information for CD25. It may not necessarily be applicable to this product.
 NCBI GI #    4557667
 NCBI GeneID    3559
 NCBI Accession #    NP_000408.1 [Other Products]
 NCBI GenBank Nucleotide #    NP_000408.1 [Other Products]
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 UniProt Primary Accession #    P01589 [Other Products]
 UniProt Secondary Accession #    Q5W007 [Other Products]
 UniProt Related Accession #    P01589 [Other Products]
 Molecular Weight    55kDa
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 NCBI Official Full Name    interleukin-2 receptor subunit alpha isoform 1
 NCBI Official Synonym Full Names    interleukin 2 receptor subunit alpha
 NCBI Official Symbol    IL2RA [Similar Products]
 NCBI Official Synonym Symbols   
p55; CD25; IL2R; IMD41; TCGFR; IDDM10
[Similar Products]
 NCBI Protein Information    interleukin-2 receptor subunit alpha
 UniProt Protein Name    Interleukin-2 receptor subunit alpha
 UniProt Synonym Protein Names   
TAC antigen; p55; CD_antigen: CD25
 UniProt Gene Name    IL2RA [Similar Products]
 UniProt Synonym Gene Names    IL-2 receptor subunit alpha; IL-2-RA; IL-2R subunit alpha; IL2-RA [Similar Products]
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 NCBI Summary for CD25    The interleukin 2 (IL2) receptor alpha (IL2RA) and beta (IL2RB) chains, together with the common gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric alpha chains (IL2RA) result in low-affinity receptor, while homodimeric beta (IL2RB) chains produce a medium-affinity receptor. Normally an integral-membrane protein, soluble IL2RA has been isolated and determined to result from extracellular proteolyisis. Alternately-spliced IL2RA mRNAs have been isolated, but the significance of each is presently unknown. Mutations in this gene are associated with interleukin 2 receptor alpha deficiency.[provided by RefSeq, Nov 2009]
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 UniProt Comments for CD25    Receptor for interleukin-2. The receptor is involved in the regulation of immune tolerance by controlling regulatory T cells (TREGs) activity. TREGs suppress the activation and expansion of autoreactive T-cells.
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Product References and Citations for anti-CD25 antibody

   T.A. Waldmann, et al, (1989) Annu Rev Biochem 58: 875-911.
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 Research Articles on CD25    1. sIL-2R could be a promising new marker for determining inflammatory disease activity in CRPS.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-CD25 antibodyPathways associated with anti-CD25 antibody
 Reference Product  PubMed Publications
 CD4 antibody  >2381 publications with CD25 and CD4
 IL2RB antibody  >767 publications with CD25 and IL2RB
 IL2 antibody  >94 publications with CD25 and IL2
 STAT3 antibody  >28 publications with CD25 and STAT3
 ICAM1 antibody  >24 publications with CD25 and ICAM1
 STAT5B antibody  >10 publications with CD25 and STAT5B
 NFKB1 antibody  >1 publications with CD25 and NFKB1
 Products by Pathway  Pathway Diagram
 Arf6 Trafficking Events Pathway antibodies  Arf6 Trafficking Events Pathway Diagram
 Calcineurin-regulated NFAT-dependent Transcription In Lymphocytes Pathway antibodies  Calcineurin-regulated NFAT-dependent Transcription In Lymphocytes Pathway Diagram
 Calcium Signaling In The CD4+ TCR Pathway antibodies  Calcium Signaling In The CD4+ TCR Pathway Diagram
 Cytokine Signaling In Immune System Pathway antibodies  Cytokine Signaling In Immune System Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Downstream Signaling In Naive CD8+ T Cells Pathway antibodies  Downstream Signaling In Naive CD8+ T Cells Pathway Diagram
 Endocytosis Pathway antibodies  Endocytosis Pathway Diagram
 Endocytosis Pathway antibodies  Endocytosis Pathway Diagram
 HTLV-I Infection Pathway antibodies  HTLV-I Infection Pathway Diagram
Diseases associated with anti-CD25 antibodyOrgans/Tissues associated with anti-CD25 antibody
 Disease Name  Pubmed Publications
 Inflammation Antibodies  >445 publications with CD25 and Inflammation
 Necrosis Antibodies  >304 publications with CD25 and Necrosis
 Lung Diseases Antibodies  >163 publications with CD25 and Lung Diseases
 Liver Diseases Antibodies  >138 publications with CD25 and Liver Diseases
 Diabetes Mellitus, Type 1 Antibodies  >132 publications with CD25 and Diabetes Mellitus, Type 1
 Multiple Sclerosis Antibodies  >112 publications with CD25 and Multiple Sclerosis
 Arthritis, Rheumatoid Antibodies  >108 publications with CD25 and Arthritis, Rheumatoid
 Vascular Diseases Antibodies  >89 publications with CD25 and Vascular Diseases
 HIV Infections Antibodies  >74 publications with CD25 and HIV Infections
 Kidney Diseases Antibodies  >73 publications with CD25 and Kidney Diseases
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >3210 publications with CD25 and Blood
 Spleen Antibodies  >353 publications with CD25 and Spleen
 Lymph Node Antibodies  >271 publications with CD25 and Lymph Node
 Bone Antibodies  >214 publications with CD25 and Bone
 Bone Marrow Antibodies  >201 publications with CD25 and Bone Marrow
 Thymus Antibodies  >195 publications with CD25 and Thymus
 Lung Antibodies  >188 publications with CD25 and Lung
 Mixed Antibodies  >149 publications with CD25 and Mixed
 Brain Antibodies  >97 publications with CD25 and Brain
 Uterus Antibodies  >26 publications with CD25 and Uterus
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