View our list of available Coronavirus (COVID-19, SARS-CoV-2, 2019-nCOV) antibodies, recombinant proteins, ELISA Kits... Options Full Datasheet Printer Friendly Datasheet Prepare for Printing Full Datasheet Printer Friendly Datasheet Prepare for Printing Have Questions? Ask Us! Request A Quote Today! Add To Compare List anti-CD252 antibody :: Mouse anti-Human CD252 Monoclonal Antibody Catalog # MBS666578 Unit / Price 0.1 mg / $425 +1 FREE 8GB USB 5x0.1 mg / $1,735 +2 FREE 8GB USB Product Name Product Info Accession #s Product Desc Diseases/Tissues/Pathways Applications References Product Name CD252, Monoclonal Antibody Popular Item Full Product Name anti-human CD252(OX40L)-Purified Preservative Free Product Synonym Names Monoclonal anti-human CD252 (CD134L, OX40L) Product Gene Name anti-CD252 antibody[Similar Products] Research Use Only For Research Use Only. Not for use in diagnostic procedures. TOP Clonality Monoclonal Isotype IgG1k Clone Number ANC10G1 Host Mouse Species Reactivity Human Purity/Purification Purified Preservative Free Form/Format 50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl Concentration 1.0 mg/ml (lot specific) Performance Cultured human umbilical cord vein endothelial cells were harvested by trypsinization, after which they were washed and incubated 45 minutes on ice with 80 ul of anti-CD134L antibody at 10 ug/ml. Cells were washed twice and incubated with 2 degree reagent Goat anti-Mouse IgG/FITC, after which they were washed three times, fixed and analyzed by FACS. A net 14% subpopulation of the cells stained positive with a mean shift of 1.0 log10 fluorescent units when compared to a Mouse IgG1 negative control at a similar concentration. Production Antibody was Protein A purified from (low FBS containing) tissue culture supernatant. Purity was >95% Immunoglobulin by SDS-PAGE and contains less than 1% Bovine Immunoglobulin. Product was 0.2u sterile filtered and vialed under aseptic conditions. Buffer 50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl. Immunogen Human HUVEC and Recombinant soluble human CD134L Preparation and Storage Store at 2 - 5 degree C. Freeze/Thawing is not recommended. Open under aseptic conditions.Product should retain activity for at least 12 months after shipping date when stored as recommended. Other Notes Small volumes of anti-CD252 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply. TOP Related Product Information for anti-CD252 antibody Information: Human CD134L (OX-40 Ligand) is a type II membrane protein with homology to TNF which is expressed on activated B cells and activated endothelial cells. CD134L binds to CD134 present on activated T cells, providing a costimulatory signal (1). This pathway seems to be more effective for costimulation of CD4+ helper T cells than for CD8+ effector T cells (4). Blockade of this interaction in mouse abrogated immunological effects in several models of inflammation and rejection (5,6,7,8). Antibody from clone ANC10G1 binds to CD134L on the cell surface of HUVEC in Flow cytometry, and blocks binding of recombinant CD134-muIg. Applications Tested/Suitable for anti-CD252 antibody Flow Cytometry (FC/FACS), ELISA (EIA), Blocking Testing Data of anti-CD252 antibody TOP Product References and Citations for anti-CD252 antibody 1) W. R. Godfrey, et al, (1994) J Exp Med 180: 757-763. 2) L.M. Higgins, et al, (1999) J Immunol 162: 486-493. 3) A.D. Weinberg, et al, (1999) J Immunol 162: 1818-1826. 4) V.Y. Taraban, et al, (2002) Eur J. Immunol. 32: 3617-3627. 5) V. Malmstrom, et al, (2001) J Immunol 166: 6972. 6) C. Nohara, et al, (2001) J Immunol 166: 2108-2115. 7) X. Yuan, et al, (2003) J Immunol 170: 2949-2955. 8) L. Tian, et al, (2002) Transplantation 74(1): 133-138. TOP Precautions All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines. Disclaimer While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information. TOP