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anti-CD276 antibody :: Mouse anti-Human CD276 / B7-H3 Monoclonal Antibody

Scan QR to view Datasheet Catalog #    MBS245290
Immunohistochemistry (IHC)
Unit / Price
0.05 mg  /  $530 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

CD276 / B7-H3, Monoclonal Antibody

★Popular Item★
 Also Known As   

Mouse Monoclonal [clone MIH42] (IgG1) to Human CD276 / B7-H3

 Product Synonym Names    Anti-CD276 / B7-H3 Antibody (clone MIH42) IHC-plus; CD276; 4Ig-B7-H3; B7 homolog 3; B7-H3; CD276 antigen; CD276 molecule; Costimulatory molecule; B7H3; B7RP-2; Human CD276; B7-H3
 Product Gene Name   

anti-CD276 antibody

[Similar Products]
 Product Synonym Gene Name    B7-H3 [Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 OMIM    605715
 3D Structure    ModBase 3D Structure for Q5ZPR3
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 Clonality    Monoclonal
 Isotype    IgG1
 Clone Number    MIH42
 Host    Mouse
 Species Reactivity    Human
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 Specificity    Detects CD276, a member of the B7 family of co-stimulatory molecules also known as B7-H3. CD276 is a type I transmembrane protein that induces the proliferation of CD4+ and CD8+ T cells, enhances the generation of cytotoxic T cells and selectively stimulates the production of interferon gamma. Expression of CD276 can be induced on dendritic cells and monocytes by inflammatory cytokines, and is also widely expressed in peripheral tissues including the heart, kidney, testis and colon. In humans, CD276 exists as two isoforms which result from gene duplication and differential splicing. CD276 is reported to have therapeutic potential for the regulation of cell-mediated immune responses to cancer, particularly in conjunction with anti-angiogenic therapy.
 Purity/Purification    Affinity Purified
 Form/Format    PBS, 0.09% sodium azide.
 Concentration    1 mg/ml (lot specific)
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 Target Species    Human
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 Preparation and Storage    +4 degree C or -20 degree C, Avoid repeated freezing and thawing.
 Other Notes    Small volumes of anti-CD276 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for anti-CD276 antibody

   B7-H3 is a member of the B7 family of proteins, which are, type I transmembrane glycoproteins containing two extracellular Ig domains. By engaging their cognate receptors on T cells, B7 family proteins provide accessory signals to T cells during T cell activation, which can be either stimulatory or inhibitory. B7-H3 is widely expressed and has been shown to be involved in bone formation via osteoblasts, allograft rejection and anti tumor response.
 Applications Tested/Suitable for anti-CD276 antibody   

Immunohistochemistry (IHC - Paraffin; IHC - Frozen), Flow Cytometry (FC/FACS)

 Application Notes for anti-CD276 antibody    Flo (1:10 - 1:100), IHC-Fr (1:100), IHC-P (10 ug/ml)
Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti...
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 Immunohistochemistry (IHC) of anti-CD276 antibody    Anti-B7-H3 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
anti-CD276 antibody Immunohistochemistry (IHC) (IHC) image
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NCBI/Uniprot data below describe general gene information for CD276. It may not necessarily be applicable to this product.
 NCBI GI #    13376852
 NCBI GeneID    80381
 NCBI Accession #    NP_079516.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_025240.2 [Other Products]
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 UniProt Primary Accession #    Q5ZPR3 [Other Products]
 UniProt Secondary Accession #    Q6P5Y4; Q6UXI2; Q8NBI8; Q8NC34; Q8NCB6; Q9BXR1 [Other Products]
 UniProt Related Accession #    Q5ZPR3 [Other Products]
 Molecular Weight    57,165 Da
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 NCBI Official Full Name    CD276 antigen isoform b
 NCBI Official Synonym Full Names    CD276 molecule
 NCBI Official Symbol    CD276 [Similar Products]
 NCBI Official Synonym Symbols   
B7H3; B7-H3; B7RP-2; 4Ig-B7-H3
[Similar Products]
 NCBI Protein Information    CD276 antigen; B7 homolog 3; costimulatory molecule
 UniProt Protein Name    CD276 antigen
 UniProt Synonym Protein Names   
4Ig-B7-H3; B7 homolog 3; B7-H3; Costimulatory molecule; CD_antigen: CD276
 Protein Family    CD276 antigen
 UniProt Gene Name    CD276 [Similar Products]
 UniProt Synonym Gene Names    B7H3; B7-H3 [Similar Products]
 UniProt Entry Name    CD276_HUMAN
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 NCBI Summary for CD276    The protein encoded by this gene belongs to the immunoglobulin superfamily, and thought to participate in the regulation of T-cell-mediated immune response. Studies show that while the transcript of this gene is ubiquitously expressed in normal tissues and solid tumors, the protein is preferentially expressed only in tumor tissues. Additionally, it was observed that the 3' UTR of this transcript contains a target site for miR29 microRNA, and there is an inverse correlation between the expression of this protein and miR29 levels, suggesting regulation of expression of this gene product by miR29. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]
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 UniProt Comments for CD276    CD276: May participate in the regulation of T-cell-mediated immune response. May play a protective role in tumor cells by inhibiting natural-killer mediated cell lysis as well as a role of marker for detection of neuroblastoma cells. May be involved in the development of acute and chronic transplant rejection and in the regulation of lymphocytic activity at mucosal surfaces. Could also play a key role in providing the placenta and fetus with a suitable immunological environment throughout pregnancy. Both isoform 1 and isoform 2 appear to be redundant in their ability to modulate CD4 T-cell responses. Isoform 2 is shown to enhance the induction of cytotoxic T-cells and selectively stimulates interferon gamma production in the presence of T-cell receptor signaling. Belongs to the immunoglobulin superfamily. BTN/MOG family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 15q23-q24

Cellular Component: integral to membrane; external side of plasma membrane

Molecular Function: protein binding; receptor binding

Biological Process: cell proliferation; positive regulation of osteoblast differentiation; regulation of immune response; negative regulation of T cell proliferation; T cell activation; negative regulation of inflammatory response; negative regulation of interleukin-2 biosynthetic process; positive regulation of T cell proliferation; immune response; positive regulation of bone mineralization; negative regulation of interferon-gamma biosynthetic process; positive regulation of interferon-gamma biosynthetic process
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 Research Articles on CD276    1. Study shows that CD276 can be used to discriminate ECs from malignant tissue from ECs from normal tissue. In addition, CD276(+) CEC do occur in higher frequencies in patients with advanced cancer.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-CD276 antibodyPathways associated with anti-CD276 antibody
 Reference Product  PubMed Publications
 CD4 antibody  >20 publications with CD276 and CD4
 CD28 antibody  >17 publications with CD276 and CD28
 CTLA4 antibody  >10 publications with CD276 and CTLA4
 CD40 antibody  >5 publications with CD276 and CD40
 CD40LG antibody  >1 publications with CD276 and CD40LG
 Products by Pathway  Pathway Diagram
 Cell Adhesion Molecules (CAMs) Pathway antibodies  Cell Adhesion Molecules (CAMs) Pathway Diagram
 Cell Adhesion Molecules (CAMs) Pathway antibodies  Cell Adhesion Molecules (CAMs) Pathway Diagram
Diseases associated with anti-CD276 antibodyOrgans/Tissues associated with anti-CD276 antibody
 Disease Name  Pubmed Publications
 Disease Models, Animal Antibodies  >14 publications with CD276 and Disease Models, Animal
 Liver Diseases Antibodies  >11 publications with CD276 and Liver Diseases
 Liver Neoplasms Antibodies  >10 publications with CD276 and Liver Neoplasms
 Inflammation Antibodies  >9 publications with CD276 and Inflammation
 Necrosis Antibodies  >9 publications with CD276 and Necrosis
 Breast Neoplasms Antibodies  >9 publications with CD276 and Breast Neoplasms
 Lung Neoplasms Antibodies  >9 publications with CD276 and Lung Neoplasms
 Genital Diseases, Male Antibodies  >7 publications with CD276 and Genital Diseases, Male
 Prostatic Diseases Antibodies  >7 publications with CD276 and Prostatic Diseases
 Carcinoma, Hepatocellular Antibodies  >6 publications with CD276 and Carcinoma, Hepatocellular
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >103 publications with CD276 and Blood
 Liver Antibodies  >16 publications with CD276 and Liver
 Lung Antibodies  >13 publications with CD276 and Lung
 Vascular Antibodies  >12 publications with CD276 and Vascular
 Bone Antibodies  >10 publications with CD276 and Bone
 Brain Antibodies  >10 publications with CD276 and Brain
 Prostate Antibodies  >7 publications with CD276 and Prostate
 Kidney Antibodies  >6 publications with CD276 and Kidney
 Heart Antibodies  >6 publications with CD276 and Heart
 Skin Antibodies  >6 publications with CD276 and Skin
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