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anti-CD28 antibody :: Mouse CD28 Monoclonal Antibody

Scan QR to view Datasheet Catalog #    MBS438728 anti-CD28 antibody
Unit / Price
0.02 mg (With BSA & Azide at 0.2mg/ml)  /  $190 +1 FREE 8GB USB
0.1 mg (With BSA & Azide at 0.2mg/ml)  /  $300 +1 FREE 8GB USB
0.1 mg (Without BSA & Azide at 1mg/ml)  /  $310 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

CD28, Monoclonal Antibody

★Popular Item★
 Also Known As   

CD28 Mouse Monoclonal Antibody

 Product Synonym Names    T-cell-specific surface glycoprotein CD28; Tp44
 Product Gene Name   

anti-CD28 antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 OMIM    186760
 3D Structure    ModBase 3D Structure for P10747
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 Clonality    Monoclonal
 Isotype    IgG2a, kappa
 Clone Number    204-12
 Host    Mouse
 Species Reactivity    Human, Pig, Cow, Sheep, Mouse
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 Specificity    Recognizes a glycoprotein of 44-88kDa, which is identified as CD28. It is the critical T-cell co-stimulatory receptor which provides to the cell the important second activation signal by binding CD80 and CD86 that are expressed by antigen presenting cells. Besides its co-stimulation role, CD28 functions in preventing T-cells from anergic hyporesponsive state or from undergoing premature apoptotic cell death. CD28 is also expressed on human fetal NK cells and some NK cell lines, whereas on murine NK cells the CD28 expression is much broader.
 Form/Format    200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
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 Cellular Localization    Cell Surface
 Immunogen    Stimulated human leucocytes
 Hu-Chromosome Location    2q33.2
 Positive Control    Jurkat cells or human lymph nodes and tonsils.
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 Preparation and Storage    Antibody with azide - store at 2 to 8 degree C. Antibody without azide - store at -20 to -80 degree C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
 Other Notes    Small volumes of anti-CD28 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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 Applications Tested/Suitable for anti-CD28 antibody   

Flow Cytometry (FC/FACS), Immunofluorescence (IF), Functional Studies

 Application Notes for anti-CD28 antibody    Flow Cytometry (0.5-1ug/million cells in 0.1ml)
Immunofluorescence (0.5-1ug/ml)
Functional Studies (Order Ab without BSA & Azide)
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NCBI/Uniprot data below describe general gene information for CD28. It may not necessarily be applicable to this product.
 NCBI GI #    340545508
 NCBI GeneID    940
 NCBI Accession #    NP_001230006.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001243077.1 [Other Products]
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 UniProt Primary Accession #    P10747 [Other Products]
 UniProt Secondary Accession #    Q13964; Q52M23; Q70WG0; Q8NI54; Q8NI55; Q8NI56; Q8WXJ2; Q9BYV0; A8KAC1 [Other Products]
 UniProt Related Accession #    P10747 [Other Products]
 Molecular Weight    44kDa (monomer); 88kDa (dimer)
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 NCBI Official Full Name    T-cell-specific surface glycoprotein CD28 isoform 2
 NCBI Official Synonym Full Names    CD28 molecule
 NCBI Official Symbol    CD28 [Similar Products]
 NCBI Official Synonym Symbols   
Tp44
[Similar Products]
 NCBI Protein Information    T-cell-specific surface glycoprotein CD28
 UniProt Protein Name    T-cell-specific surface glycoprotein CD28
 UniProt Synonym Protein Names   
TP44; CD_antigen: CD28
 Protein Family    T-cell-specific surface glycoprotein
 UniProt Gene Name    CD28 [Similar Products]
 UniProt Entry Name    CD28_HUMAN
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 NCBI Summary for CD28    The protein encoded by this gene is essential for T-cell proliferation and survival, cytokine production, and T-helper type-2 development. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jul 2011]
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 UniProt Comments for CD28    CD28: a type I plasma membrane protein Involved in T-cell activation, the induction of cell proliferation and cytokine production and promotion of T-cell survival. Binds to B7-1 and -2. Expressed in T cells and plasma cells, but not in less mature B cells. Six splice-variant isoforms have been described.

Protein type: Cell surface; Membrane protein, integral

Chromosomal Location of Human Ortholog: 2q33

Cellular Component: cell surface; cytosol; external side of plasma membrane; integral to plasma membrane; plasma membrane

Molecular Function: coreceptor activity; phosphatidylinositol-4,5-bisphosphate 3-kinase activity; protease binding; protein binding; SH3/SH2 adaptor activity

Biological Process: cell surface receptor linked signal transduction; cytokine biosynthetic process; humoral immune response; phosphoinositide-mediated signaling; positive regulation of interleukin-10 production; positive regulation of interleukin-2 biosynthetic process; positive regulation of interleukin-4 production; positive regulation of mitosis; positive regulation of T cell proliferation; regulation of defense response to virus by virus; regulation of phosphoinositide 3-kinase cascade; regulatory T cell differentiation; T cell costimulation
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Product References and Citations for anti-CD28 antibody

   In Leucocyte typing Workshop VI. Sandilands, G. et al. Clinical and Experimental Immunology, 162(3), 516-27 (2010)
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 Research Articles on CD28    1. in complex with T cell receptor, promotes glycolysis
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-CD28 antibodyPathways associated with anti-CD28 antibody
 Reference Product  PubMed Publications
 CD80 antibody  >1886 publications with CD28 and CD80
 CD86 antibody  >1140 publications with CD28 and CD86
 LCK antibody  >180 publications with CD28 and LCK
 VAV1 antibody  >64 publications with CD28 and VAV1
 ITK antibody  >48 publications with CD28 and ITK
 GRB2 antibody  >31 publications with CD28 and GRB2
 GRAP2 antibody  >7 publications with CD28 and GRAP2
 CD247 antibody  >1 publications with CD28 and CD247
 Products by Pathway  Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 Allograft Rejection Pathway antibodies  Allograft Rejection Pathway Diagram
 Allograft Rejection Pathway antibodies  Allograft Rejection Pathway Diagram
 Allograft Rejection Pathway antibodies  Allograft Rejection Pathway Diagram
 Autoimmune Thyroid Disease Pathway antibodies  Autoimmune Thyroid Disease Pathway Diagram
 Autoimmune Thyroid Disease Pathway antibodies  Autoimmune Thyroid Disease Pathway Diagram
 CD28 Co-stimulation Pathway antibodies  CD28 Co-stimulation Pathway Diagram
 CD28 Dependent PI3K/Akt Signaling Pathway antibodies  CD28 Dependent PI3K/Akt Signaling Pathway Diagram
 CD28 Dependent Vav1 Pathway antibodies  CD28 Dependent Vav1 Pathway Diagram
 Cell Adhesion Molecules (CAMs) Pathway antibodies  Cell Adhesion Molecules (CAMs) Pathway Diagram
Diseases associated with anti-CD28 antibodyOrgans/Tissues associated with anti-CD28 antibody
 Disease Name  Pubmed Publications
 Autoimmune Diseases Antibodies  >1314 publications with CD28 and Autoimmune Diseases
 Inflammation Antibodies  >904 publications with CD28 and Inflammation
 Necrosis Antibodies  >893 publications with CD28 and Necrosis
 HIV Infections Antibodies  >469 publications with CD28 and HIV Infections
 Arthritis, Rheumatoid Antibodies  >386 publications with CD28 and Arthritis, Rheumatoid
 Lung Diseases Antibodies  >345 publications with CD28 and Lung Diseases
 Cardiovascular Diseases Antibodies  >341 publications with CD28 and Cardiovascular Diseases
 Vascular Diseases Antibodies  >310 publications with CD28 and Vascular Diseases
 Lymphoma Antibodies  >310 publications with CD28 and Lymphoma
 Carcinoma Antibodies  >259 publications with CD28 and Carcinoma
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >8571 publications with CD28 and Blood
 Thymus Antibodies  >354 publications with CD28 and Thymus
 Brain Antibodies  >137 publications with CD28 and Brain
 Embryonic Tissue Antibodies  >56 publications with CD28 and Embryonic Tissue
 Pharynx Antibodies  >36 publications with CD28 and Pharynx
 Connective Tissue Antibodies  >29 publications with CD28 and Connective Tissue
 Placenta Antibodies  >21 publications with CD28 and Placenta
 Uterus Antibodies  >19 publications with CD28 and Uterus
 Trachea Antibodies  >11 publications with CD28 and Trachea
 Adipose Tissue Antibodies  >10 publications with CD28 and Adipose Tissue
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