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anti-CD3 antibody :: Rat CD3 Monoclonal Antibody

Scan QR to view Datasheet Catalog #    MBS211042
Unit / Price
0.1 mg  /  $185 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

CD3, Monoclonal Antibody

 Also Known As   

RAT ANTI MOUSE CD3:FITC

 Product Gene Name   

anti-CD3 antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Clonality    Monoclonal
 Isotype    IgG2a
 Clone Number    KT3
 Host    Rat
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 Form/Format    FITC
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
 Concentration    IgG concentration 0.1 mg/ml (lot specific)
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 Perservative Stabilisers    0.09% Sodium Azide
1% Bovine Serum Albumin
 Buffer Solution    Phosphate buffered saline
 Target Species    Mouse
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 Preparation and Storage    Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.
 Other Notes    Small volumes of anti-CD3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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 Applications Tested/Suitable for anti-CD3 antibody   

Flow cytometry (FC/FACS)

 Application Notes for anti-CD3 antibody    Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood. The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR.
Flow Cytometry: Minimum Dilution: Neat; Maximum Dilution: 1/10
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 Testing Data of anti-CD3 antibody    Staining of mouse spleen with Rat anti Mouse CD3 Epsilon (T3): RPE - Alexa Fluor 647
anti-CD3 antibody Testing Data image
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 Testing Data of anti-CD3 antibody    Staining of mouse spleen cells with Rat anti Mouse CD3 Epsilon (T3):RPE (MBS213843)
anti-CD3 antibody Testing Data image
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 Testing Data of anti-CD3 antibody    Staining of mouse spleen with Rat anti Mouse CD3 Epsilon (T3): RPE - Alexa Fluor 750
anti-CD3 antibody Testing Data image
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 Testing Data of anti-CD3 antibody    Immunoperoxidase staining of Mouse lymph node cryosection with Rat anti Mouse CD3 antibody, clone KT3 followed by horseradish peroxidase conjugated Goat anti Rat IgG (MBS235195) as a detection reagent. Low power
anti-CD3 antibody Testing Data image
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 Testing Data of anti-CD3 antibody    Immunoperoxidase staining of Mouse spleen cryosection with Rat anti Mouse CD3 antibody, clone KT3 followed by horseradish peroxidase conjugated Goat anti Rat IgG (MBS235195) as a detection reagent. Low power
anti-CD3 antibody Testing Data image
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 Testing Data of anti-CD3 antibody    Immunoperoxidase staining of Mouse lymph node cryosection with Rat anti Mouse CD3 antibody, clone KT3 followed by horseradish peroxidase conjugated Goat anti Rat IgG (MBS235195) as a detection reagent. Low power
anti-CD3 antibody Testing Data image
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 Testing Data of anti-CD3 antibody    Immunoperoxidase staining of Mouse spleen cryosection with Rat anti Mouse CD3 antibody, clone KT3 followed by horseradish peroxidase conjugated Goat anti Rat IgG (MBS235195) as a detection reagent. Medium power
anti-CD3 antibody Testing Data image
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 Testing Data of anti-CD3 antibody    Immunoperoxidase staining of Mouse lymph node cryosection with Rat anti Mouse CD3 antibody, clone KT3 followed by horseradish peroxidase conjugated Goat anti Rat IgG (MBS235195) as a detection reagent. Low power
anti-CD3 antibody Testing Data image
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 Testing Data of anti-CD3 antibody    Staining of mouse peripheral blood lymphocytes with Rat anti Mouse CD3 Epsilon (T3): endotoxin low (MBS224425)
anti-CD3 antibody Testing Data image
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 Testing Data of anti-CD3 antibody    Staining of mouse spleen cells with Rat anti Mouse CD3 Epsilon (T3): APC (MBS210024)
anti-CD3 antibody Testing Data image
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NCBI/Uniprot data below describe general gene information for CD3. It may not necessarily be applicable to this product.
 NCBI GI #    6680886
 NCBI GeneID    12501
 NCBI Accession #    NP_031674.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_007648.4 [Other Products]
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 UniProt Related Accession #    P22646 [Other Products]
 Molecular Weight    21,393 Da
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 NCBI Official Full Name    T-cell surface glycoprotein CD3 epsilon chain
 NCBI Official Synonym Full Names    CD3 antigen, epsilon polypeptide
 NCBI Official Symbol    Cd3e [Similar Products]
 NCBI Official Synonym Symbols   
CD3; T3e; AI504783; CD3epsilon
[Similar Products]
 NCBI Protein Information    T-cell surface glycoprotein CD3 epsilon chain; T-cell surface antigen T3/Leu-4 epsilon chain
 UniProt Protein Name    T-cell surface glycoprotein CD3 epsilon chain
 UniProt Synonym Protein Names   
T-cell surface antigen T3/Leu-4 epsilon chain; CD_antigen: CD3e
 Protein Family    CD302 antigen
 UniProt Gene Name    Cd3e [Similar Products]
 UniProt Entry Name    CD3E_MOUSE
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 UniProt Comments for CD3    CD3E: a T cell surface glycoprotein that is a component of the T cell antigen receptor. The recruitment of Nck by CD3 epsilon reveals a ligand-induced conformational change essential for T cell receptor signaling and synapse formation. Contains 1 immunoglobulin-like domain and 1 ITAM domain.

Protein type: Receptor, misc.; Membrane protein, integral

Cellular Component: T cell receptor complex; membrane; integral to membrane; plasma membrane; immunological synapse; alpha-beta T cell receptor complex; intercellular junction; external side of plasma membrane

Molecular Function: protein binding; signal transducer activity; transmembrane receptor activity; protein heterodimerization activity; SH3 domain binding

Biological Process: positive regulation of interleukin-2 biosynthetic process; lymphocyte activation; positive regulation of calcium-mediated signaling; T cell receptor signaling pathway; negative thymic T cell selection; positive regulation of interleukin-4 production; positive regulation of interferon-gamma production; positive regulation of peptidyl-tyrosine phosphorylation; cell surface receptor linked signal transduction; negative regulation of smoothened signaling pathway; T cell costimulation; positive regulation of T cell proliferation; positive regulation of alpha-beta T cell proliferation; positive regulation of T cell activation; positive regulation of T cell anergy; response to nutrient
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 Research Articles on CD3    1. these results suggest that CD3zeta can be degraded by two pathways: SLAP/c-Cbl, which targets internalized cell surface CD3zeta dependent on TCR signaling, and LAPTM5, which targets intracellular CD3zeta independent of TCR signaling.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with anti-CD3 antibodyDiseases associated with anti-CD3 antibody
 Products by Pathway  Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 Chagas Disease (American Trypanosomiasis) Pathway antibodies  Chagas Disease (American Trypanosomiasis) Pathway Diagram
 Chagas Disease (American Trypanosomiasis) Pathway antibodies  Chagas Disease (American Trypanosomiasis) Pathway Diagram
 Costimulation By The CD28 Family Pathway antibodies  Costimulation By The CD28 Family Pathway Diagram
 Downstream TCR Signaling Pathway antibodies  Downstream TCR Signaling Pathway Diagram
 Generation Of Second Messenger Molecules Pathway antibodies  Generation Of Second Messenger Molecules Pathway Diagram
 HTLV-I Infection Pathway antibodies  HTLV-I Infection Pathway Diagram
 HTLV-I Infection Pathway antibodies  HTLV-I Infection Pathway Diagram
 Hematopoietic Cell Lineage Pathway antibodies  Hematopoietic Cell Lineage Pathway Diagram
 Hematopoietic Cell Lineage Pathway antibodies  Hematopoietic Cell Lineage Pathway Diagram
 Disease Name  Pubmed Publications
 Lymphoma Antibodies  >26 publications with CD3 and Lymphoma
 Inflammation Antibodies  >20 publications with CD3 and Inflammation
 Disease Models, Animal Antibodies  >16 publications with CD3 and Disease Models, Animal
 Immunologic Deficiency Syndromes Antibodies  >14 publications with CD3 and Immunologic Deficiency Syndromes
 Necrosis Antibodies  >13 publications with CD3 and Necrosis
 Neoplasms, Experimental Antibodies  >7 publications with CD3 and Neoplasms, Experimental
 Severe Combined Immunodeficiency Antibodies  >5 publications with CD3 and Severe Combined Immunodeficiency
 Carcinoma Antibodies  >5 publications with CD3 and Carcinoma
 Lung Diseases Antibodies  >5 publications with CD3 and Lung Diseases
 Arthritis, Rheumatoid Antibodies  >5 publications with CD3 and Arthritis, Rheumatoid
Organs/Tissues associated with anti-CD3 antibody
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >190 publications with CD3 and Blood
 Thymus Antibodies  >46 publications with CD3 and Thymus
 Spleen Antibodies  >35 publications with CD3 and Spleen
 Bone Marrow Antibodies  >26 publications with CD3 and Bone Marrow
 Mammary Gland Antibodies  >1 publications with CD3 and Mammary Gland
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