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anti-CD33 antibody :: Rabbit anti-Human CD33 Monoclonal Antibody

Scan QR to view Datasheet Catalog #    MBS370338
Unit / Price
5 + Control Slides  /  $130 +1 FREE 8GB USB
3 ml (Prediluted)  /  $280 +1 FREE 8GB USB
0.1 mL (Concentrate)  /  $305 +1 FREE 8GB USB
7 ml (Prediluted)  /  $475 +1 FREE 8GB USB
0.5 mL (Concentrate)  /  $740 +1 FREE 8GB USB
15 ml (Prediluted)  /  $815 +1 FREE 8GB USB
1 mL (Concentrate)  /  $1,085 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

CD33, Monoclonal Antibody

★Popular Item★
 Also Known As   

CD33 Rmab

 Product Gene Name   

anti-CD33 antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 OMIM    M23197 mRNA
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 Clonality    Monoclonal
 Isotype    IgG
 Clone Number    RBT-CD33
 Host    Rabbit
 Species Reactivity    Human
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 Intended Use    This antibody is intended for use in Immunohistochemical applications on formalinfixed paraffin-embedded tissues (FFPE), frozen tissue sections and cell preparations. Interpretation of results should be performed by a qualified medical professional.
 Immunogen    Recombinant protein encoding the extracellular domain of human CD33.
 Summary and Explanation    CD33 (gp62 or siglec-3) is a glycosylated transmembrane protein that is a member of the sialic acid-binding immunoglobulin-like lectin (siglec) family. The genomic locus of this protein has been mapped to chromosome 19q13.1-3.5. The function of CD33 is not known, but it may have a role in cell-to-cell adhesion. In maturing granulocytic cells, there is progressive down-regulation of CD33 from the blast stage to mature neutrophils. However, in monocytes and macrophages/histiocytes, strong expression of CD33 is maintained throughout maturation.

Detection of CD33 using monoclonal antibodies has been a critical component in immunophenotyping acute leukemias, particularly Acute Myeloid Leukemias.
 Localization    Membranous
 Control    Placenta, Myometrium, Lung, Colon, Spleen, Lymph Node, Tonsil, Acute Myeloid Leukemia
 Presentation    CD33 is a rabbit monoclonal antibody derived from cell culture supernatant that is concentrated, dialyzed, filter sterilized and diluted in buffer pH 7.5, containing BSA and sodium azide as a preservative.
 Precautions    1. This product contains sodium azide (NaN3), a toxic chemical which may react with plumbing to form highly explosive build-ups of metal azides. Upon disposal, flush with large volumes of water to prevent sodium azide build-up.
2. Ensure proper handling procedures are used with reagent. Always wear proper laboratory equipment such as laboratory coat and gloves when handling reagents.
3. Unused solution should be disposed of according to local and federal regulations.
4. Do not ingest reagent. If reagent ingested, contact a poison control center immediately.
5. For complete recommendations for handling biological specimens please refer to the CDC document, “Guidelines for Safe Work Practices in Human and Animal Medical Diagnostic Laboratories” (5).
 Specimen Preparation    Paraffin sections: The antibody can be used on formalin-fixed paraffin-embedded (FFPE) tissue sections. Ensure tissue undergoes appropriate fixation to ensure best results. Pre-treatment of tissues with heat-induced epitope retrieval (HIER) is recommended using ImmunoDNA Retriever with Citrate (Please Inquire), ImmunoDNA Retriever with EDTA (Please Inquire) or ImmunoDNA Digestor (Please Inquire). See reverse side for complete protocol. Tissue should remain hydrated via use Immuno/DNA Washer solutions (Please Inquire).
Frozen sections and cell preparations: The antibody can be used for labeling acetone-fixed frozen sections and acetone-fixed cell preparations.
 Staining Procedure    1. Cut and mount 3-5 micron formalin-fixed paraffin-embedded tissues on positive charged slides such as Hydrophilic Plus Slides (Please Inquire).
2. Air dry for 2 hours at 58° C.
3. Deparaffinize, dehydrate and rehydrate tissues.
4. Subject tissues to heat epitope retrieval using a suitable retrieval solution such as ImmunoDNA Retriever with Citrate (Please Inquire) or EDTA (Please Inquire).
5. Any of three heating methods may be used:
a. TintoRetriever Pressure Cooker or Equivalent
Place tissues/slides in a staining dish or coplin jar containing the ImmunoDNA Retriever with Citrate or EDTA, and place in the pressure cooker. Add 1-2 inches of distilled water to the pressure cooker and turn heat to high. Incubate for 15 minutes. Open and immediately transfer slides to room temperature.
b. TintoRetriever PT Module or Water Bath Method
Place tissues/slides in a pre-warmed staining dish or coplin jar containing the ImmunoDNA Retriever with Citrate or EDTA at 95°-99° C. Incubate for 30-60 minutes.
c. Conventional Steamer Method
Place tissues/slides in a pre-warmed staining dish or coplin jar containing the ImmunoDNA Retriever with Citrate or EDTA in a Steamer, cover and steam for 30-60 minutes.
6. After heat treatment, transfer slides in ImmunoDNA Retriever with Citrate or EDTA to room temperature and let stand for 15-20 minutes.
7. For manual staining, perform antibody incubation at ambient temperature. For automated staining methods, perform antibody incubation according to instrument manufacturer’s instructions.
8. Wash slides with IHC wash buffer or DI water.
9. Continue IHC staining protocol.
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 Preparation and Storage    Store at 2-8 °C. Do not use after expiration date listed on package label. Temperature fluctuations should be avoided. Store appropriately when not in use, and avoid prolonged exposure to room temperature conditions.
 Other Notes    Small volumes of anti-CD33 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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 Immunohistochemistry of anti-CD33 antibody    Inset: IHC of CD33 on a FFPE Colon Tissue
anti-CD33 antibody Immunohistochemistry (IHC) image
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 Immunohistochemistry Protocol of anti-CD33 antibody    anti-CD33 antibody Immunohistochemistry Protocol (IHC) image
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NCBI/Uniprot data below describe general gene information for CD33. It may not necessarily be applicable to this product.
 NCBI GI #    1032656012
 NCBI GeneID    945
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 UniProt Secondary Accession #    Q8TD24; B4E3P8; C9JEN7; F8WAL2 [Other Products]
 UniProt Related Accession #    P20138 [Other Products]
 Molecular Weight    25,293 Da
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 NCBI Official Full Name    CD33 molecule transcript variant
 NCBI Official Synonym Full Names    CD33 molecule
 NCBI Official Symbol    CD33 [Similar Products]
 NCBI Official Synonym Symbols   
p67; SIGLEC3; SIGLEC-3
[Similar Products]
 NCBI Protein Information    myeloid cell surface antigen CD33
 UniProt Protein Name    Myeloid cell surface antigen CD33
 UniProt Synonym Protein Names   
Sialic acid-binding Ig-like lectin 3; Siglec-3; gp67; CD_antigen: CD33
 Protein Family    Myeloid cell surface antigen
 UniProt Gene Name    CD33 [Similar Products]
 UniProt Synonym Gene Names    SIGLEC3; Siglec-3 [Similar Products]
 UniProt Entry Name    CD33_HUMAN
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 UniProt Comments for CD33    CD33: Putative adhesion molecule of myelomonocytic-derived cells that mediates sialic-acid dependent binding to cells. Preferentially binds to alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. In the immune response, may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase(s) via their SH2 domain(s) that block signal transduction through dephosphorylation of signaling molecules. Induces apoptosis in acute myeloid leukemia (in vitro). Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family.

Protein type: Cell adhesion; Membrane protein, integral

Chromosomal Location of Human Ortholog: 19q13.3

Cellular Component: external side of plasma membrane; integral to plasma membrane; plasma membrane

Molecular Function: protein binding; receptor activity

Biological Process: cell-cell signaling; negative regulation of cell proliferation; regulation of immune response; signal transduction
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 Research Articles on CD33    1. CD33-expressing microglia play a central role in the development of leukoencephalopathy in Nasu-Hakola disease brains
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-CD33 antibodyPathways associated with anti-CD33 antibody
 Reference Product  PubMed Publications
 CD34 antibody  >1126 publications with CD33 and CD34
 CD19 antibody  >497 publications with CD33 and CD19
 CD7 antibody  >394 publications with CD33 and CD7
 CD2 antibody  >244 publications with CD33 and CD2
 NCAM1 antibody  >65 publications with CD33 and NCAM1
 CEBPA antibody  >10 publications with CD33 and CEBPA
 PTPN11 antibody  >10 publications with CD33 and PTPN11
 FUT4 antibody  >2 publications with CD33 and FUT4
 ANPEP antibody  >1 publications with CD33 and ANPEP
 Products by Pathway  Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 Hematopoietic Cell Lineage Pathway antibodies  Hematopoietic Cell Lineage Pathway Diagram
 Hematopoietic Cell Lineage Pathway antibodies  Hematopoietic Cell Lineage Pathway Diagram
 Immune System Pathway antibodies  Immune System Pathway Diagram
 Immunoregulatory Interactions Between A Lymphoid And A Non-Lymphoid Cell Pathway antibodies  Immunoregulatory Interactions Between A Lymphoid And A Non-Lymphoid Cell Pathway Diagram
 Innate Immune System Pathway antibodies  Innate Immune System Pathway Diagram
 Neutrophil Degranulation Pathway antibodies  Neutrophil Degranulation Pathway Diagram
Diseases associated with anti-CD33 antibodyOrgans/Tissues associated with anti-CD33 antibody
 Disease Name  Pubmed Publications
 Leukemia, Myeloid, Acute Antibodies  >1258 publications with CD33 and Leukemia, Myeloid, Acute
 Cell Transformation, Neoplastic Antibodies  >100 publications with CD33 and Cell Transformation, Neoplastic
 Necrosis Antibodies  >96 publications with CD33 and Necrosis
 Inflammation Antibodies  >93 publications with CD33 and Inflammation
 Death Antibodies  >92 publications with CD33 and Death
 Cardiovascular Diseases Antibodies  >88 publications with CD33 and Cardiovascular Diseases
 Alzheimer Disease Antibodies  >59 publications with CD33 and Alzheimer Disease
 Respiratory Tract Diseases Antibodies  >57 publications with CD33 and Respiratory Tract Diseases
 Breast Neoplasms Antibodies  >45 publications with CD33 and Breast Neoplasms
 Lung Diseases Antibodies  >44 publications with CD33 and Lung Diseases
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >1534 publications with CD33 and Blood
 Bone Antibodies  >1063 publications with CD33 and Bone
 Bone Marrow Antibodies  >1048 publications with CD33 and Bone Marrow
 Liver Antibodies  >100 publications with CD33 and Liver
 Lymph Node Antibodies  >69 publications with CD33 and Lymph Node
 Lung Antibodies  >61 publications with CD33 and Lung
 Brain Antibodies  >48 publications with CD33 and Brain
 Thymus Antibodies  >30 publications with CD33 and Thymus
 Kidney Antibodies  >23 publications with CD33 and Kidney
 Prostate Antibodies  >14 publications with CD33 and Prostate
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