View our list of available Coronavirus (COVID-19, SARS-CoV-2, 2019-nCOV) antibodies, recombinant proteins, ELISA Kits... Options Full Datasheet Printer Friendly Datasheet Prepare for Printing Full Datasheet Printer Friendly Datasheet Prepare for Printing Have Questions? Ask Us! Request A Quote Today! Add To Compare List anti-CD33 antibody :: Rabbit anti-Human CD33 Monoclonal Antibody Catalog # MBS370338 Unit / Price 5 Slides / $200 +1 FREE 8GB USB 3 mL (RTU) / $410 +1 FREE 8GB USB 0.1 mL (Concentrate) / $440 +1 FREE 8GB USB 7 mL (RTU) / $675 +1 FREE 8GB USB 0.5 mL (Concentrate) / $1,045 +1 FREE 8GB USB 15 mL (RTU) / $1,140 +1 FREE 8GB USB 1 mL (Concentrate) / $1,530 +1 FREE 8GB USB 5x15 mL (RTU) / $4,965 +6 FREE 8GB USB 5x1 mL (Concentrate) / $6,710 +8 FREE 8GB USB Previous Next Product Name Product Info Accession #s Product Desc Diseases/Tissues/Pathways Applications References Product Name CD33, Monoclonal Antibody Popular Item Full Product Name CD33 Rmab Product Gene Name anti-CD33 antibody[Similar Products] Research Use Only For Research Use Only. Not for use in diagnostic procedures. TOP OMIM M23197 mRNA Clonality Monoclonal Isotype IgG Clone Number RBT-CD33 Host Rabbit Species Reactivity Human Intended Use This antibody is intended for use in Immunohistochemical applications on formalinfixed paraffin-embedded tissues (FFPE), frozen tissue sections and cell preparations. Interpretation of results should be performed by a qualified medical professional. Immunogen Recombinant protein encoding the extracellular domain of human CD33. Summary and Explanation CD33 (gp62 or siglec-3) is a glycosylated transmembrane protein that is a member of the sialic acid-binding immunoglobulin-like lectin (siglec) family. The genomic locus of this protein has been mapped to chromosome 19q13.1-3.5. The function of CD33 is not known, but it may have a role in cell-to-cell adhesion. In maturing granulocytic cells, there is progressive down-regulation of CD33 from the blast stage to mature neutrophils. However, in monocytes and macrophages/histiocytes, strong expression of CD33 is maintained throughout maturation.Detection of CD33 using monoclonal antibodies has been a critical component in immunophenotyping acute leukemias, particularly Acute Myeloid Leukemias. Localization Membranous Control Placenta, Myometrium, Lung, Colon, Spleen, Lymph Node, Tonsil, Acute Myeloid Leukemia Presentation CD33 is a rabbit monoclonal antibody derived from cell culture supernatant that is concentrated, dialyzed, filter sterilized and diluted in buffer pH 7.5, containing BSA and sodium azide as a preservative. Precautions 1. This product contains sodium azide (NaN3), a toxic chemical which may react with plumbing to form highly explosive build-ups of metal azides. Upon disposal, flush with large volumes of water to prevent sodium azide build-up. 2. Ensure proper handling procedures are used with reagent. Always wear proper laboratory equipment such as laboratory coat and gloves when handling reagents. 3. Unused solution should be disposed of according to local and federal regulations. 4. Do not ingest reagent. If reagent ingested, contact a poison control center immediately. 5. For complete recommendations for handling biological specimens please refer to the CDC document, “Guidelines for Safe Work Practices in Human and Animal Laboratories” (5). Specimen Preparation Paraffin sections: The antibody can be used on formalin-fixed paraffin-embedded (FFPE) tissue sections. Ensure tissue undergoes appropriate fixation to ensure best results. Pre-treatment of tissues with heat-induced epitope retrieval (HIER) is recommended using ImmunoDNA Retriever with Citrate (Please Inquire), ImmunoDNA Retriever with EDTA (Please Inquire) or ImmunoDNA Digestor (Please Inquire). See reverse side for complete protocol. Tissue should remain hydrated via use Immuno/DNA Washer solutions (Please Inquire). Frozen sections and cell preparations: The antibody can be used for labeling acetone-fixed frozen sections and acetone-fixed cell preparations. Staining Procedure 1. Cut and mount 3-5 micron formalin-fixed paraffin-embedded tissues on positive charged slides such as Hydrophilic Plus Slides (Please Inquire). 2. Air dry for 2 hours at 58° C. 3. Deparaffinize, dehydrate and rehydrate tissues. 4. Subject tissues to heat epitope retrieval using a suitable retrieval solution such as ImmunoDNA Retriever with Citrate (Please Inquire) or EDTA (Please Inquire).5. Any of three heating methods may be used:a. TintoRetriever Pressure Cooker or EquivalentPlace tissues/slides in a staining dish or coplin jar containing the ImmunoDNA Retriever with Citrate or EDTA, and place in the pressure cooker. Add 1-2 inches of distilled water to the pressure cooker and turn heat to high. Incubate for 15 minutes. Open and immediately transfer slides to room temperature.b. TintoRetriever PT Module or Water Bath MethodPlace tissues/slides in a pre-warmed staining dish or coplin jar containing the ImmunoDNA Retriever with Citrate or EDTA at 95°-99° C. Incubate for 30-60 minutes.c. Conventional Steamer Method Place tissues/slides in a pre-warmed staining dish or coplin jar containing the ImmunoDNA Retriever with Citrate or EDTA in a Steamer, cover and steam for 30-60 minutes. 6. After heat treatment, transfer slides in ImmunoDNA Retriever with Citrate or EDTA to room temperature and let stand for 15-20 minutes.7. For manual staining, perform antibody incubation at ambient temperature. For automated staining methods, perform antibody incubation according to instrument manufacturer’s instructions.8. Wash slides with IHC wash buffer or DI water.9. Continue IHC staining protocol. Preparation and Storage Store at 2-8 °C. Do not use after expiration date listed on package label. Temperature fluctuations should be avoided. Store appropriately when not in use, and avoid prolonged exposure to room temperature conditions. ISO Certification Manufactured in an ISO 13485:2016 Certified Laboratory. Other Notes Small volumes of anti-CD33 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply. TOP Immunohistochemistry of anti-CD33 antibody Inset: IHC of CD33 on a FFPE Colon Tissue Immunohistochemistry Protocol of anti-CD33 antibody TOP NCBI/Uniprot data below describe general gene information for CD33. It may not necessarily be applicable to this product. NCBI GI # 1032656012 NCBI GeneID 945 UniProt Secondary Accession # Q8TD24; B4E3P8; C9JEN7; F8WAL2[Other Products] UniProt Related Accession # P20138[Other Products] Molecular Weight 25,293 Da TOP NCBI Official Full Name CD33 molecule transcript variant NCBI Official Synonym Full Names CD33 molecule NCBI Official Symbol CD33 [Similar Products] NCBI Official Synonym Symbols p67; SIGLEC3; SIGLEC-3 [Similar Products] NCBI Protein Information myeloid cell surface antigen CD33 UniProt Protein Name Myeloid cell surface antigen CD33 UniProt Synonym Protein Names Sialic acid-binding Ig-like lectin 3; Siglec-3; gp67; CD_antigen: CD33 Protein Family Myeloid cell surface antigen UniProt Gene Name CD33 [Similar Products] UniProt Synonym Gene Names SIGLEC3; Siglec-3 [Similar Products] UniProt Entry Name CD33_HUMAN TOP TOP UniProt Comments for CD33 CD33: Putative adhesion molecule of myelomonocytic-derived cells that mediates sialic-acid dependent binding to cells. Preferentially binds to alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. In the immune response, may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase(s) via their SH2 domain(s) that block signal transduction through dephosphorylation of signaling molecules. Induces apoptosis in acute myeloid leukemia (in vitro). Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family.Protein type: Cell adhesion; Membrane protein, integralChromosomal Location of Human Ortholog: 19q13.3Cellular Component: external side of plasma membrane; integral to plasma membrane; plasma membraneMolecular Function: protein binding; receptor activityBiological Process: cell-cell signaling; negative regulation of cell proliferation; regulation of immune response; signal transduction Research Articles on CD33 1. CD33-expressing microglia play a central role in the development of leukoencephalopathy in Nasu-Hakola disease brains TOP Precautions All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines. Disclaimer While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information. TOP Products associated with anti-CD33 antibody Reference Product PubMed Publications CD34 antibody >1126 publications with CD33 and CD34 CD19 antibody >497 publications with CD33 and CD19 CD7 antibody >394 publications with CD33 and CD7 CD2 antibody >244 publications with CD33 and CD2 NCAM1 antibody >65 publications with CD33 and NCAM1 CEBPA antibody >10 publications with CD33 and CEBPA PTPN11 antibody >10 publications with CD33 and PTPN11 FUT4 antibody >2 publications with CD33 and FUT4 ANPEP antibody >1 publications with CD33 and ANPEP Pathways associated with anti-CD33 antibody Products by Pathway Pathway Diagram Adaptive Immune System Pathway antibodies Adaptive Immune System Pathway Diagram Hematopoietic Cell Lineage Pathway antibodies Hematopoietic Cell Lineage Pathway Diagram Hematopoietic Cell Lineage Pathway antibodies Hematopoietic Cell Lineage Pathway Diagram Immune System Pathway antibodies Immune System Pathway Diagram Immunoregulatory Interactions Between A Lymphoid And A Non-Lymphoid Cell Pathway antibodies Immunoregulatory Interactions Between A Lymphoid And A Non-Lymphoid Cell Pathway Diagram Innate Immune System Pathway antibodies Innate Immune System Pathway Diagram Neutrophil Degranulation Pathway antibodies Neutrophil Degranulation Pathway Diagram Diseases associated with anti-CD33 antibody Disease Name Pubmed Publications Leukemia, Myeloid, Acute Antibodies >1258 publications with CD33 and Leukemia, Myeloid, Acute Cell Transformation, Neoplastic Antibodies >100 publications with CD33 and Cell Transformation, Neoplastic Necrosis Antibodies >96 publications with CD33 and Necrosis Inflammation Antibodies >93 publications with CD33 and Inflammation Death Antibodies >92 publications with CD33 and Death Cardiovascular Diseases Antibodies >88 publications with CD33 and Cardiovascular Diseases Alzheimer Disease Antibodies >59 publications with CD33 and Alzheimer Disease Respiratory Tract Diseases Antibodies >57 publications with CD33 and Respiratory Tract Diseases Breast Neoplasms Antibodies >45 publications with CD33 and Breast Neoplasms Lung Diseases Antibodies >44 publications with CD33 and Lung Diseases Organs/Tissues associated with anti-CD33 antibody Organ/Tissue Name Pubmed Publications Blood Antibodies >1534 publications with CD33 and Blood Bone Antibodies >1063 publications with CD33 and Bone Bone Marrow Antibodies >1048 publications with CD33 and Bone Marrow Liver Antibodies >100 publications with CD33 and Liver Lymph Node Antibodies >69 publications with CD33 and Lymph Node Lung Antibodies >61 publications with CD33 and Lung Brain Antibodies >48 publications with CD33 and Brain Thymus Antibodies >30 publications with CD33 and Thymus Kidney Antibodies >23 publications with CD33 and Kidney Prostate Antibodies >14 publications with CD33 and Prostate TOP