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anti-CD335 antibody :: Mouse CD335 Monoclonal Antibody

Scan QR to view Datasheet Catalog #    MBS213799
Unit / Price
100 Tests  /  $590 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

CD335, Monoclonal Antibody

 Also Known As   

MOUSE ANTI BOVINE CD335:RPE

 Product Gene Name   

anti-CD335 antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 3D Structure    ModBase 3D Structure for Q863H2
 Clonality    Monoclonal
 Isotype    IgG1
 Clone Number    AKS1
 Host    Mouse
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 Form/Format    Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilised
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 Reconstitution    Reconstitute with 1 ml distilled water
Perservative Stabilisers
 Immunogen    Fusion protein consisting of the extracellular region of bovine CD335.
Fusion Partners
 Target Species    Bovine
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 Preparation and Storage    Prior to reconstitution store at 4 degree C. Following reconstitution store at 4 degree C. DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 12 months from date of reconstitution.
 Other Notes    Small volumes of anti-CD335 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Product Description specifically for anti-CD335 antibody

   Mouse anti Bovine CD335 antibody, clone AKS1 recognizes bovine CD335, also known as NKp46 and Natural cytotoxicity triggering receptor 1. CD335 is a type I transmembrane protein, with two extracellular C2-type immunoglobulin-like domains, which functions as an activating receptor. CD335 is expressed by human natural killer cells (Sivori et al. 1997). The bovine homologue is expressed on bovine NK cells (Storset et al. 2004) and no expression of CD335 has been detected on B cells, T cells, monocytes or granulocytes. Clone AKS1 is reported to activate lysis of FcgammaR-expressing cell line P815, by IL-2 activated NKp46+ cells (Storset et al. 2004).
 Applications Tested/Suitable for anti-CD335 antibody   

Flow cytometry (FC/FACS)

 Application Notes for anti-CD335 antibody    Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry: Minimum Dilution: Neat
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 Testing Data of anti-CD335 antibody    Published customer image: Oenothein B Primes bovine CD335+ cells to respond to IL-18. (A) Bovine PBMCs (105 cells/well) were depleted of CD335+ cells and treated with 20 ug/ml oenothein B or X-VIVO medium alone for 24 hrs. Cells were then washed and treated with 10 ng/ml rhu IL-18 or medium alone for 18 hrs. After incubation, IFN? levels in the supernatant fluids were measured by ELISA. The data are expressed as mean +/- SEM of three independent experiments comparing depleted PBMCs to un-depleted controls tested concurrently. All samples were tested in duplicate or triplicate. Statistical significance was measured by Two-way ANOVA with Bonferroni post-test. *p
anti-CD335 antibody Testing Data image
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 Testing Data of anti-CD335 antibody    Published customer image: Effect of anti-CD2 mAbs on bovine NK cell functions. (A) IFN-? response from NK cells caused by cross-linking of CD2. Plastic wells were pre-coated with two different mAbs against bovine CD2, along with NKp46-mAb as positive control and CD8-mAb or medium as negative controls. rbIL-2 activated NK cells were added and incubated for 24 h in the presence or absence of rbIL-12 (0.5 U/ml), and IFN-? content in the supernatants analyzed by an ELISA. Data shown are means +/- SD of two to four individual animals. (B-C) IL-2 activated NK cell killing of the bovine target lines BL3.1 and MDBK in a 4 h 51Cr-release assay, with addition of the indicated mAbs for blocking. CD2mAb I = BAQ95A, CD2 mAb II = MUC2A, CD8 mAb = CACT80C, NKp46 mAb = AKS1. (D) As above, using murine Fc-receptor bearing P815 target cells to obtain a redirected lysis effect. Data shown in (B-D) are from one animal representative of three to five.From: Boysen et al. BMC Immunology 2006 7:10.
anti-CD335 antibody Testing Data image
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 Testing Data of anti-CD335 antibody    Staining of bovine peripheral blood lymphocytes with Mouse anti Bovine CD335 (MBS224513) followed by Goat anti Mouse IgG (H/L):FITC (MBS235142). A small positive peak representing NK cells can be identified
anti-CD335 antibody Testing Data image
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 Testing Data of anti-CD335 antibody    Published customer image: Oenothein B induces IL-2Ra or CD69 on bovine and human NK cells. (A) Bovine PBMCs (105 cells/well) were treated with 20 ug/ml oenothein B in X-VIVO medium for 24 hrs, and IL-2Ra expression on NK cells was measured by multi-color flow cytometry. Representative examples of two-color flow cytometry plots comparing IL-2Ra staining on oenothein B-treated and untreated bovine NK cells (CD335+) from each animal are shown. (B) Human PBMCs (105 cells/well) were treated with 40 ug/ml oenothein B in cRPMI medium for 48 hrs. CD69 expression on NK cells was then measured by flow cytometry. Representative examples of two-color flow cytometry plots comparing CD69 staining on oenothein B-treated and untreated human NK cells from each donor are shown.From: Ramstead AG, Schepetkin IA, Quinn MT, Jutila MA (2012) Oenothein B, a Cyclic Dimeric Ellagitannin Isolated from Epilobium angustifolium, Enhances IFN? Production by Lymphocytes. PLoS ONE 7(11): e50546.
anti-CD335 antibody Testing Data image
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 Testing Data of anti-CD335 antibody    Published customer image: CD2 distribution on bovine NK cells in peripheral blood and during cell culture. (A) CD2 expression by PBMCs from healthy cattle, and in NKp46-selected cells cultured for the indicated time in the presence of 100U/ml rbIL-2. Results were obtained by two-colour flow cytometry, gating for viable lymphocytes. Data shown are from one animal representative of 19. (B) Monitoring of cell divisions of CD2- and CD2+ NK cell populations during three and six days of rbIL-2 (100U/ml) stimulation. NKp46-selected cells were labelled with CFSE the day after isolation and CFSE intensity (filled histograms) measured in flow cytometry on the indicated days, gating for CD2- or CD2+ viable cells. Solid line open histograms show non-dividing NK cells, cultured in the presence of 1U/ml rbIL-2, while broken line open histograms show unlabeled stimulated NK cells. Data shown are from one animal representative of five. (C) Stability of CD2 expression in NK cell subsets cultured separately. NK cells separated with immunomagnetic beads for absence or presence of CD2 were cultured separately for eleven days in the presence of IL-2, monitoring CD2 expression at three different time points (filled histograms). Day 1 corresponds to the day of separation, 24 h after primary NK cell isolation. Open histograms show secondary mAb controls. Data shown are from one animal representative of eight.From: Boysen et al. BMC Immunology 2006 7:10.
anti-CD335 antibody Testing Data image
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NCBI/Uniprot data below describe general gene information for CD335. It may not necessarily be applicable to this product.
 NCBI GI #    34365789
 NCBI GeneID    369024
 NCBI Accession #    NP_899209.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_183365.1 [Other Products]
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 UniProt Primary Accession #    Q863H2 [Other Products]
 UniProt Related Accession #    Q863H2 [Other Products]
 Molecular Weight    34,494 Da
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 NCBI Official Full Name    natural cytotoxicity triggering receptor 1
 NCBI Official Symbol    NCR1 [Similar Products]
 NCBI Official Synonym Symbols   
NKP46
[Similar Products]
 NCBI Protein Information    natural cytotoxicity triggering receptor 1; NK-p46; natural killer cell p46-related protein
 UniProt Protein Name    Natural cytotoxicity triggering receptor 1
 UniProt Synonym Protein Names   
Natural killer cell p46-related protein; NK-p46; NKp46; CD_antigen: CD335
 UniProt Gene Name    NCR1 [Similar Products]
 UniProt Synonym Gene Names    NK-p46; NKp46 [Similar Products]
 UniProt Entry Name    NCTR1_BOVIN
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 UniProt Comments for CD335    Cytotoxicity activating receptor that may contribute to the increased efficiency of activated natural killer (NK) cells to mediate tumor cell lysis.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with anti-CD335 antibodyDiseases associated with anti-CD335 antibody
 Products by Pathway  Pathway Diagram
 Natural Killer Cell Mediated Cytotoxicity Pathway antibodies  Natural Killer Cell Mediated Cytotoxicity Pathway Diagram
 Natural Killer Cell Mediated Cytotoxicity Pathway antibodies  Natural Killer Cell Mediated Cytotoxicity Pathway Diagram
 Disease Name  Pubmed Publications
 Inflammation Antibodies  >20 publications with CD335 and Inflammation
 Liver Diseases Antibodies  >17 publications with CD335 and Liver Diseases
 Necrosis Antibodies  >15 publications with CD335 and Necrosis
 Disease Models, Animal Antibodies  >10 publications with CD335 and Disease Models, Animal
 Skin Diseases Antibodies  >10 publications with CD335 and Skin Diseases
 Nervous System Diseases Antibodies  >8 publications with CD335 and Nervous System Diseases
 Lung Diseases Antibodies  >8 publications with CD335 and Lung Diseases
 Skin Neoplasms Antibodies  >6 publications with CD335 and Skin Neoplasms
 Neoplasm Metastasis Antibodies  >5 publications with CD335 and Neoplasm Metastasis
 Cell Transformation, Neoplastic Antibodies  >5 publications with CD335 and Cell Transformation, Neoplastic
Organs/Tissues associated with anti-CD335 antibody
 Organ/Tissue Name  Pubmed Publications
 Intestine Antibodies  >24 publications with CD335 and Intestine
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