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anti-CD357 antibody :: Mouse anti-Human CD357 Monoclonal Antibody

Scan QR to view Datasheet Catalog #    MBS666414
Testing Data
Unit / Price
120 Tests  /  $440 +1 FREE 8GB USB
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 Product Name   

CD357, Monoclonal Antibody

 Also Known As   

anti-human CD357(GITR)-FITC

 Product Synonym Names    Monoclonal anti-human CD357 (GITR,AITR)/FITC (IgM isotype)
 Product Gene Name   

anti-CD357 antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Clonality    Monoclonal
 Isotype    IgMk
 Clone Number    ANC7D6-1B7
 Host    Mouse
 Species Reactivity    Human
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 Form/Format    50 mM Sodium Phosphate pH 7.5, 500 mM Potassium Chloride, 150mM NaCl, 5% Glycerol, 0.2% BSA.
 Concentration    Volume: 0.2 ml (lot specific)
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 Performance    Ficoll prepared human peripheral blood mononuclear cells (PBMC) were stimulated 5 days in culture with PHA. Five x 105 cells per tube were washed and pre incubated 5 minutes with 20 ul of 250 ug/ml human IgG (to block non specific binding) after which they were incubated 45 minutes on ice with 80 ul of anti-GITR/FITC at a 1:50 dilution factor (10ug/ml). Cells were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 1.03 log10 fluorescent units when compared to a Mouse IgM/FITC negative control at a similar concentration. Binding was blocked when cells were pre incubated with a 10-fold excess of unlabeled anti-GITR mAb.
 Conjugation    FITC
 Production    Antibody from (low FBS containing) tissue culture supernatant was purified by size exclusion chromatographand reacted with FITC. Unconjugated FITC was removed from conjugate using a desalting column. Antibody conjugate is at 0.5 mg/ml with a Fluorescein/IgM molar ratio of 35.
 Buffer    50 mM Sodium Phosphate pH 7.5, 500 mM Potassium Chloride, 150mM NaCl, 5% Glycerol, 0.2% BSA, 0.04% NaN3 (as a preservative).
 Immunogen    Recombinant human GITR, PHA stimulated human PBL
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 Preparation and Storage    Store at 2 - 5 degree C. Freeze thawing not recommended. Protect from light.
Product should retain activity for at least 12 months after shipping date when stored as recommended.
 Other Notes    Small volumes of anti-CD357 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for anti-CD357 antibody

   Information: Glucocorticoid induced Tumor Necrosis Factor receptor family related gene (CD357, GITR, AITR) is a type 1 228 amino acid transmembrane protein that has been designated TNFRSF18. GITR is expressed mainly by CD4+ CD25+ T lymphocytes. Its engagement by antibodies or its ligand (GITRL) induces a costimulatory signal which can break T cell suppression (2). GITR has been implicated as a regulatory mechanism in anti-parasitic immune resposes (4), and is involved with Macrophages activation in atherosclerotic plaque formation (3). Clone ANC7D6-1E7 binds to human CD357 in EIA and on stimulated cell surface in FACS. Pre incubation of the receptor with an excess of this mAb will block binding of recombinant GITR Ligand.
 Applications Tested/Suitable for anti-CD357 antibody   

Flow Cytometry (FC/FACS), ELISA (EIA)

 Application Notes for anti-CD357 antibody    1:50 (or use 1.6 ul of concentrated reagent per 5 x 105-cell test)
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 Testing Data of anti-CD357 antibody    anti-CD357 antibody Testing Data image
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Product References and Citations for anti-CD357 antibody

   1) Gurney AL, et al. (1999) Curr Biol 9(4): 215-218. 2) Ji HB, et al. (2004) J Immunol 172(10): 5823-5827. 3) Kim WJ, WH Lee, et al. (2006) Immunology 119(3): 44-9. 4) Furze RC, Selkirk ME, et al. (2006) Microbes Infect 8(12-13): 2803-10. 5) Kohm AP, SD Miller, et al. (2004) J Immunol 172: 4686-4690. 6) McHugh, R.S. et al. (2002) Immunity 16:311. 7) Shin, H.H. et al. (2002) FEBS Lett. 514:275. 8). Shimizu, J. et al. (2002) Nature Immunol. 3:135.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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