Specificity
Mouse CD3epsilon (Mr 25 kDa)
CD3epsilon, a member of the immunoglobulin superfamily of cell surface receptors, is comprised of five invariable chains ranging in size from 16-28 kDa and is closely associated with the T cell antigen receptor (TCR). It is expressed on all T cells of all mouse strains. CD3 plays a major role in signaling during antigen recognition, leading to T-cell activation. The 145-2C11 monoclonal antibody recognizes an epitope on the 25kD epsilon chain of the CD3/TCR complex. In the presence of Fc receptor-bearing accessory cells, soluble 145-2C11 can activate primed and naïve T cell in vitro. 145-2C11 can also induce redirected lysis of specific target cells by CTL clones and it can block lysis of specific target cells by antigen-specific CTL's. Immobilized 145-2C11 can activate both normal T lymphocytes and cloned T cell lines. Under certain conditions, T cell activation by 145-2C11 may result in apoptotic cell death.
Warning
Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Applications Tested/Suitable for
anti-CD3e-BIMA antibody
Identification and enumeration of CD3+ cells by Flow Cytometry, Immunoprecipitation, Western Blot, In vitro depletion of CD3+ cells, In vitro activation of T cells, Immunohistochemistry
Product References and Citations for
anti-CD3e-BIMA antibody
1. Leo, O., M. Foo, D. Sachs, L. Samuelson, and J. Bluestone. 1987. Proc. Natl. Acad. Sci. USA 84:1374.
2. Portoles, P., J. Rojo, A. Golby, M. Bonneville, S. Gromkowski, L. Greenbaum, C. A. Janeway, Jr., D. B. Murphy, and K. Bottomly. 1989. J. Immunol. 142:4169.
3. Kruisbeek, A.M., and E. Shevach. 1991. In: Currents Protocols in Immunology. J. Coligan, A. Kruisbeek, D. Margulies, E.M. Shevach, and W. Strober, eds. John Wiley & Sons, New York, pp. 3.12.1-3.12.14.
4. Duke, R.C., J.J. Cohen, S.A. Boehme, M.J. Lenardo, C.D. Surh, H. Kishimoto, and J. Sprent. 1995. In: Currents Protocols in Immunology. J. Coligan, A. Kruisbeek, D. Margulies, E.M. Shevach, and W. Strober, eds. John Wiley & Sons, New York, pp. 3.17.1-3.17.33.
5. Ucker, D.S., J. Meyers, and P.D. Obermiller. 1992. J. Immunol. 149:1583.
6. Wang, R., K.M. Murphy, D.Y. Loh, C. Weaver, and J.H. Russell. 1993. J. Immunol. 150:3832.
7. Salvadori, S., B. Gansbacher, A.M. Pizzimenti, and K.S. Zier. 1994. J. Immunol. 153.5176.
8. Payer, E., A. Elbe, and G. Stingl., 1991. J. Immunol. 146:2536.
Precautions
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