Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
1.855.MyBioSource
Fax 1.858.633.0166    Email sales@mybiosource.com 
tel/fax
menu1 Home Products Ordering Company Help menu7
bottom
   MyBioSource rightarrow Antibody rightarrow CD3e rightarrow Monoclonal CD3e  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Menu separator
separator
ELISA Kit ELISA Kit
CLIA Kit CLIA Kit
PCR Kit PCR Kit
Monoclonal Antibody Monoclonal Antibody
Polyclonal Antibody Polyclonal Antibody
Secondary Antibody Secondary Antibody
Antigen Antigen
Biochemical Biochemical
cDNA Clone cDNA Clone
siRNA siRNA
Peptide Peptide
Recombinant/Purified Protein Rec./Purified Protein

Custom ELISA Kit Custom ELISA Kit
Custom Protein Custom Protein
Custom Antibody Custom Antibody
Antibody Matched Pairs Antibody Matched Pairs
Antibody & Corresponding Blocking Peptide Pairs Antibody Peptide Pairs
Phospho-Specific Antibodies Phospho Antibodies
Products by Disease Products by Disease
Products by Pathway Products by Pathway
Products by Tissue Products by Tissue

arrow Advanced Search
arrow Submit Technical Q&A
arrow International Distributors
arrow Contact Us
separator
Our Best Sellers moreseparator
separator
 • Prominin 1 (PROM1) Antibody
 • Lipoprotein Lipase, LPL ELISA Kit
 • Cold-inducible RNA-binding protein (CIRBP) ELISA Kit
 • Nitric Oxide Non-Enzymatic Assay Kit
 • Netrin 1 (Ntn1) ELISA Kit
 • IKK-gamma (IKBKG) Antibody
 • Alpha-2 Macroglobulin (A2M) ELISA Kit
 • DNA Lambda C1857 S7 Vector
 • SGK1 Antibody
 • Procollagen Type I N-Terminal Propeptide (PINP) ELISA Kit
 • PDCD2L Antibody
 • Akt (AKT1) Antibody
 • anti-Guinea Pig IgA Antibody
 • CD47 molecule ELISA Kit
 downarrow more ...
separator
separator
DatasheetFull DatasheetPrinter Friendly DatasheetPrint This DatasheetAdd to Compare ListHave Questions? Ask UsRequest for a Quotation today

anti-CD3e antibody :: Mouse CD3e Monoclonal Antibody

Scan QR to view Datasheet Catalog #    MBS4380305
Immunohistochemistry (IHC)
Unit / Price
0.02 mg (With BSA & Azide at 0.2mg/ml)  /  $190 +1 FREE 8GB USB
0.1 mg (With BSA & Azide at 0.2mg/ml)  /  $300 +1 FREE 8GB USB
0.1 mg (Without BSA at 1mg/ml)  /  $310 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

CD3e, Monoclonal Antibody

★Popular Item★
 Also Known As   

CD3e (T-Cell Marker)

 Product Synonym Names    CD 3E; CD3 epsilon; CD3 TCR complex; CD3E; CD3e antigen epsilon polypeptide (TiT3 complex); T cell antigen receptor complex epsilon subunit of T3; T-cell surface antigen T3/Leu-4 epsilon chain; T-cell surface glycoprotein CD3 epsilon chain; T3E; TCRE; TiT3 complex
 Product Gene Name   

anti-CD3e antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
Table BarTOPTable Bar
 3D Structure    ModBase 3D Structure for P07766
 Clonality    Monoclonal
 Isotype    IgG1
 Clone Number    PC3/188A
 Host    Mouse
 Species Reactivity    Human. Others not known.
Section Bar
 Specificity    Recognizes the -chain of CD3, which consists of five different polypeptide chains (designated as gamma, delta, epsilon, zeta, and eta) with MW ranging from 16-28kDa. The CD3 complex is closely associated at the lymphocyte cell surface with the T cell antigen receptor (TCR). Reportedly, CD3 complex is involved in signal transduction to the T cell interior following antigen recognition. The CD3 antigen is first detectable in early thymocytes and probably represents one of the earliest signs of commitment to the T cell lineage. In cortical thymocytes, CD3 is predominantly intra-cytoplasmic. However, in medullary thymocytes, it appears on the T cell surface. CD3 antigen is a highly specific marker for T cells, and is present in majority of T cell neoplasms.
 Form/Format    200ug/ml of Ab purified from rabbit anti-serum by Protein A. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA at 1.0mg/ml.
Section Bar
 Immunogen    A synthetic peptide corresponding to amino acids 156-168 of the cytoplasmic domain of human CD3-? chain
 Cellular Localization    Cell Surface and Cytoplasmic
 Hu-chromosome Location    11q23.3
 Positive Control    Jurkat cells. Tonsil and lymph node
Section Bar
 Preparation and Storage    Antibody with azide - store at 2 to 8 degree C.
Antibody without azide - store at -20 to -80 degree C.
Antibody is stable for 24 months. Non-hazardous. No MSDS required.
 Other Notes    Small volumes of anti-CD3e antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Table BarTOPTable Bar
 Applications Tested/Suitable for anti-CD3e antibody   

Flow Cytometry (FC/FACS), Immunofluorescence (IF), Western Blot (WB), Immunohistochemistry (IHC) Formalin

 Application Notes for anti-CD3e antibody    FC/FACS: 0.5-1ug/million cells
IF: 1-2ug/ml
WB: 1-2ug/ml for 60 minutes at RT
IHC-F: 1-2ug/ml for 30 minutes at RT; Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
Section Bar
 Immunohistochemistry (IHC) of anti-CD3e antibody    Formalin-fixed, paraffin-embedded human Tonsil stained with CD3 epsilon Monoclonal Antibody (PC3/188A).
anti-CD3e antibody Immunohistochemistry (IHC) (IHC) image
Table BarTOPTable Bar
NCBI/Uniprot data below describe general gene information for CD3e. It may not necessarily be applicable to this product.
 NCBI GI #    4502671
 NCBI GeneID    916
 NCBI Accession #    NP_000724.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_000733.3 [Other Products]
Section Bar
 UniProt Primary Accession #    P07766 [Other Products]
 UniProt Secondary Accession #    A8K997 [Other Products]
 UniProt Related Accession #    P07766 [Other Products]
 Molecular Weight    20kDa
Table BarTOPTable Bar
 NCBI Official Full Name    T-cell surface glycoprotein CD3 epsilon chain
 NCBI Official Synonym Full Names    CD3e molecule
 NCBI Official Symbol    CD3E [Similar Products]
 NCBI Official Synonym Symbols   
T3E; TCRE; IMD18
[Similar Products]
 NCBI Protein Information    T-cell surface glycoprotein CD3 epsilon chain
 UniProt Protein Name    T-cell surface glycoprotein CD3 epsilon chain
 UniProt Synonym Protein Names   
T-cell surface antigen T3/Leu-4 epsilon chain; CD_antigen: CD3e
 Protein Family    T-cell surface glycoprotein
 UniProt Gene Name    CD3E [Similar Products]
 UniProt Synonym Gene Names    T3E [Similar Products]
Section Bar
 NCBI Summary for CD3e    The protein encoded by this gene is the CD3-epsilon polypeptide, which together with CD3-gamma, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T-cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development. Defects in this gene cause immunodeficiency. This gene has also been linked to a susceptibility to type I diabetes in women. [provided by RefSeq, Jul 2008]
Section Bar
 UniProt Comments for CD3e    Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways (PubMed:2470098). In addition of this role of signal transduction in T-cell activation, CD3E plays an essential role in correct T-cell development. Initiates the TCR-CD3 complex assembly by forming the two heterodimers CD3D/CD3E and CD3G/CD3E. Participates also in internalization and cell surface down-regulation of TCR-CD3 complexes via endocytosis sequences present in CD3E cytosolic region (PubMed:10384095, PubMed:26507128).
Table BarTOPTable Bar
 

Product References and Citations for anti-CD3e antibody

   Cibull ML et. al. Histopathology, 1989, 15(6):599-605. Mason DY et. al. Journal of Clinical Pathology, 1989, 42(11):1194-200.
Section Bar
 Research Articles on CD3e    1. The ionic CD3-epsilon -Lck interaction controls the phosphorylation level of the T-cell receptor.
Table BarTOPTable Bar
 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Table BarTOPTable Bar
Products associated with anti-CD3e antibodyPathways associated with anti-CD3e antibody
 Reference Product  PubMed Publications
 CD4 antibody  >67 publications with CD3e and CD4
 LCK antibody  >17 publications with CD3e and LCK
 ZAP70 antibody  >16 publications with CD3e and ZAP70
 CD3D antibody  >13 publications with CD3e and CD3D
 CD8A antibody  >8 publications with CD3e and CD8A
 CD3G antibody  >8 publications with CD3e and CD3G
 SYK antibody  >4 publications with CD3e and SYK
 CD247 antibody  >2 publications with CD3e and CD247
 Products by Pathway  Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 CXCR4-mediated Signaling Events Pathway antibodies  CXCR4-mediated Signaling Events Pathway Diagram
 Chagas Disease (American Trypanosomiasis) Pathway antibodies  Chagas Disease (American Trypanosomiasis) Pathway Diagram
 Chagas Disease (American Trypanosomiasis) Pathway antibodies  Chagas Disease (American Trypanosomiasis) Pathway Diagram
 Costimulation By The CD28 Family Pathway antibodies  Costimulation By The CD28 Family Pathway Diagram
 Downstream TCR Signaling Pathway antibodies  Downstream TCR Signaling Pathway Diagram
 Downstream Signaling In Naive CD8+ T Cells Pathway antibodies  Downstream Signaling In Naive CD8+ T Cells Pathway Diagram
 Generation Of Second Messenger Molecules Pathway antibodies  Generation Of Second Messenger Molecules Pathway Diagram
 HTLV-I Infection Pathway antibodies  HTLV-I Infection Pathway Diagram
 HTLV-I Infection Pathway antibodies  HTLV-I Infection Pathway Diagram
Diseases associated with anti-CD3e antibodyOrgans/Tissues associated with anti-CD3e antibody
 Disease Name  Pubmed Publications
 Lymphoma Antibodies  >26 publications with CD3e and Lymphoma
 Inflammation Antibodies  >20 publications with CD3e and Inflammation
 Disease Models, Animal Antibodies  >17 publications with CD3e and Disease Models, Animal
 Immunologic Deficiency Syndromes Antibodies  >14 publications with CD3e and Immunologic Deficiency Syndromes
 Necrosis Antibodies  >13 publications with CD3e and Necrosis
 Neoplasms, Experimental Antibodies  >7 publications with CD3e and Neoplasms, Experimental
 Severe Combined Immunodeficiency Antibodies  >5 publications with CD3e and Severe Combined Immunodeficiency
 Carcinoma Antibodies  >5 publications with CD3e and Carcinoma
 Lung Diseases Antibodies  >5 publications with CD3e and Lung Diseases
 Arthritis, Rheumatoid Antibodies  >5 publications with CD3e and Arthritis, Rheumatoid
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >191 publications with CD3e and Blood
 Thymus Antibodies  >47 publications with CD3e and Thymus
 Spleen Antibodies  >35 publications with CD3e and Spleen
 Lymph Node Antibodies  >16 publications with CD3e and Lymph Node
 Liver Antibodies  >12 publications with CD3e and Liver
 Intestine Antibodies  >10 publications with CD3e and Intestine
 Brain Antibodies  >6 publications with CD3e and Brain
 Lung Antibodies  >6 publications with CD3e and Lung
 Kidney Antibodies  >6 publications with CD3e and Kidney
 Heart Antibodies  >4 publications with CD3e and Heart
Table BarTOPTable Bar
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions