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| Product Name
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CD4, Monoclonal Antibody
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| Also Known As
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RAT ANTI MOUSE CD4:RPE
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| Product Gene Name
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[Similar Products]
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| Research Use Only
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For Research Use Only. Not for use in diagnostic procedures.
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| 3D Structure
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ModBase 3D Structure for P06332
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| Clonality
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Monoclonal
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| Isotype
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IgG2a
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| Clone Number
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YTS177.9
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| Host
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Rat
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| Form/Format
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Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilised
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| Reconstitution
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Reconstitute with 1 ml distilled water
Perservative Stabilisers
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| Immunogen
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Mouse spleen cells
Fusion Partners
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| Target Species
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Mouse
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| Preparation and Storage
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Prior to reconstitution store at 4 degree C. Following reconstitution store at 4 degree C. DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 12 months from date of reconstitution.
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| Other Notes
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Small volumes of anti-CD4 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Product Description specifically for anti-CD4 antibody
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Rat anti Mouse CD4 antibody, clone YTS177.9 reacts with the Mouse CD4 antigen, non polymorphic epitope (Qin et al. 1990). Rat anti Mouse CD4 antibody, Rat anti Mouse CD4 antibody, clone YTS177.9 reacts with CD4 transfectants (Cobbold et al. 1990).Rat anti Mouse CD4 antibody, clone YTS177.9 also blocks MHC-II dependant T-cell responses in vitro and in vivo and induces tolerance (Qin et al. 1990) and Cobbold et al. 1990).
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| Applications Tested/Suitable for anti-CD4 antibody
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Flow cytometry (FC/FACS)
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| Application Notes for anti-CD4 antibody
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Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells or 100ul of whole blood.The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR.
Flow Cytometry: Maximum Dilution: Neat
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| Testing Data of anti-CD4 antibody
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Staining of mouse peripheral blood lymphocytes with Rat anti Mouse CD4:FITC (MBS210616)
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| NCBI/Uniprot data below describe general gene information for CD4. It may not necessarily be applicable to this product. |
| NCBI GI #
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7304953
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| NCBI GeneID
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12504
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| NCBI Accession #
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NP_038516.1
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[Other Products]
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| NCBI GenBank Nucleotide #
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NM_013488.2
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[Other Products]
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| UniProt Primary Accession #
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P06332
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[Other Products]
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| UniProt Related Accession #
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P06332
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[Other Products]
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| Molecular Weight
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24,473 Da
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TOP
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| NCBI Official Full Name
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T-cell surface glycoprotein CD4
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| NCBI Official Synonym Full Names
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CD4 antigen
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| NCBI Official Symbol
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Cd4
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[Similar Products]
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| NCBI Official Synonym Symbols
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L3T4; Ly-4
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[Similar Products]
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| NCBI Protein Information
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T-cell surface glycoprotein CD4; T-cell differentiation antigen L3T4; T-cell surface antigen T4/Leu-3; T-cell surface glycoprotein CD4 precursor (T-cell surface antigen T4/Leu-3) (T-cell differentiation antigen L3T4)
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| UniProt Protein Name
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T-cell surface glycoprotein CD4
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| UniProt Synonym Protein Names
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T-cell differentiation antigen L3T4; T-cell surface antigen T4/Leu-3; CD_antigen: CD4
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| Protein Family
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CD40 ligand
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| UniProt Gene Name
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Cd4
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[Similar Products]
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| UniProt Entry Name
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CD4_MOUSE
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| UniProt Comments for CD4
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CD4: Accessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. Induces the aggregation of lipid rafts. Associates with LCK. Binds to HIV-1 gp120 and to P4HB/PDI and upon HIV-1 binding to the cell membrane, is part of P4HB/PDI- CD4-CXCR4-gp120 complex. Interacts with HIV-1 Envelope polyprotein gp160 and protein Vpu. Interacts with Human Herpes virus 7 capsid proteins. Interacts with PTK2/FAK1; this interaction requires the presence of HIV-1 gp120.
Protein type: Membrane protein, integral; Cell surface
Cellular Component: endoplasmic reticulum membrane; cell surface; membrane; endoplasmic reticulum lumen; plasma membrane; integral to membrane; lipid raft; external side of plasma membrane
Molecular Function: protein binding; enzyme binding; protein homodimerization activity; zinc ion binding; coreceptor activity; glycoprotein binding; protein kinase binding
Biological Process: maintenance of cellular protein localization; T cell activation; immune system process; cytokine production; T cell selection; positive regulation of calcium-mediated signaling; induction by virus of cell-cell fusion in host; defense response to Gram-negative bacterium; positive regulation of peptidyl-tyrosine phosphorylation; cell surface receptor linked signal transduction; positive regulation of protein kinase activity; protein palmitoleylation; immune response; positive regulation of T cell activation; cell adhesion; regulation of T cell activation; T cell differentiation
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| Research Articles on CD4
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1. This study establishes an important role of IgE in abdominal aortic aneurysms pathogenesis by activating CD4+ T cells, mast cells, and macrophages.
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| Precautions
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All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
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| Disclaimer
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While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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| Pathways associated with anti-CD4 antibody | Diseases associated with anti-CD4 antibody |
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| Organs/Tissues associated with anti-CD4 antibody |
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Antibody 
