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Rat anti-Mouse CD49b/Pan NK Cell Monoclonal Antibody

Scan QR to view Datasheet Catalog #    MBS673571 anti-CD49b/Pan NK Cell antibody
Unit / Price
0.2 mg  /  $320 +1 FREE 8GB USB
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 Product Name   

CD49b/Pan NK Cell, Monoclonal Antibody

 Also Known As   

Rat Anti-Mouse CD49b/VLA-2a-PE

 Product Synonym Names    Rat Anti-Mouse CD49b/VLA-2a - Phycoerythrin-Labeled; Rat Anti-Mouse CD49b/Pan NK Cell
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Clonality    Monoclonal
 Isotype    Rat (Lewis) IgM kappa
 Clone Number    DX5
 Host    Rat
 Species Reactivity    Mouse
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 Specificity    Mouse CD49b, Mr 160-kDa
The monoclonal antibody DX5 reacts with CD49b, also known as very late antigen-2 (VLA-2) and alpha 2 integrin.1 The antibody stains the majority of NK cells and a small subpopulation of T cells in all mouse strains tested (e.g., A/J, AKR, BALB/c, C3H/HeJ, C57BL/6, C57BL/10, C57BR, C58, CBA/Ca, CBA/J, DBA/1, DBA/2, SJL, SWR).2. 3 Three-color flow cytometry analysis has revealed that most cells that express NK-1.1 (NKR-P1C) also express CD49b. However, small subsets of DX5+NK-1.1- and DX5-NK- 1.1+ cells can be found, especially among the CD3+ population of T lymphocytes.2 NK cells cultured in the presence of IL-2 progressively lose reactivity with DX5 as a consequence of cellular proliferation.1 Therefore, DX5 can be used to define functionally distinct subsets of murine NK cells. The monoclonal antibody DX5 has not been demonstrated to have activating or blocking activity.2
 Form/Format    R - phycoerythrin (R - PE) Conjugate
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 Immunogen    RIL-2-propagated NK1.1^+ cells from C57BL/6 mice
 Fusion Partner    Mouse SP2/0 myeloma cell line
 Characterization    To ensure lot-to-lot consistency, each batch of monoclonal antibody is tested by flow cytometry to conform to characteristics of a standard reference reagent. Representative data are included in this product insert.
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 Preparation and Storage    The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2- 8 degree C.
The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The R-phycoerythrin (R-PE) conjugate is supplied as 0.1 mg in 1.0 mL or 0.2 mg in 2.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
The Allophycocyanin (APC) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
The Allophycocyanin-Cyanine 7 (APC-CY 7) conjugates are supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
The low endotoxin, azide-free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of PBS. Aliquot and store at or below -20 degree C.
Protect conjugated forms from light. Aliquot and freeze the low endotoxin, azide-free product at -20 degree C immediately upon receipt. Each reagent is stable for the period shown on the bottle label if stored as directed.
 Other Notes    Small volumes of anti-CD49b/Pan NK Cell antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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 Applications Tested/Suitable for anti-CD49b/Pan NK Cell antibody   

Flow Cytometry

 Application Notes for anti-CD49b/Pan NK Cell antibody    Research Application(s): The DX5 antibody is not suitable for immunohistochemical staining of either frozen or paraffin sections.

Flow Cytometry Suggested Dilution: <= 0.2 ug/10^6 cells
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Product References and Citations for anti-CD49b/Pan NK Cell antibody

   1. Arase, H., T. Saito, J.H. Phillips, and L.L. Lanier. 2001. J. Immunol. 167:1141-1144.
2. Abrams, J. Personal communication
3. Moore, T.A., U. von Freeden-Jeffry, R. Muray, and A. Zlotnik. 1996. J. Immunol. 157:2366-2373.
4. Lazetic, S. and J.H. Phillips. Unpublished observations.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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