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anti-CD53 antibody :: Mouse CD53 Monoclonal Antibody

Scan QR to view Datasheet Catalog #    MBS439289 anti-CD53 antibody
Unit / Price
0.02 mg (With BSA & Azide at 0.2mg/ml)  /  $190 +1 FREE 8GB USB
0.1 mg (With BSA & Azide at 0.2mg/ml)  /  $300 +1 FREE 8GB USB
0.1 mg (Without BSA & Azide at 1mg/ml)  /  $310 +1 FREE 8GB USB
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

CD53, Monoclonal Antibody

★Popular Item★
 Also Known As   

CD53 (TSPAN25) Mouse Monoclonal Antibody

 Product Synonym Names    CD53 glycoprotein; CD53 tetraspan antigen; Cell surface glycoprotein CD53; Leukocyte surface antigen CD53; Tetraspanin-25 (Tspan-25); TSPAN25
 Product Gene Name   

anti-CD53 antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 OMIM    151525
 3D Structure    ModBase 3D Structure for P19397
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 Clonality    Monoclonal
 Isotype    IgG2b, kappa
 Clone Number    63-5A3
 Host    Mouse
 Species Reactivity    Human.
Others not tested
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 Specificity    Recognizes a protein of 33-55kDa, identified as CD53 (Workshop V; Code CD53.1). CD53 is expressed on monocytes, and macrophages, granulocytes, dendritic cells, osteoblasts and osteoclasts, NK cells, and on T- and B-cells from every stage of differentiation but is absent from platelets, erythrocytes, and non-haemopoietic cells. CD53 is a member of a family of tetraspan transmembrane proteins, including CD9, CD37, CD63, CD81, and CD82. It associates with integrins, MHC class II molecules, and a tyrosine phosphatase and plays a role in cellular activation as part of a signal transduction complex involving other membrane glycoproteins. Defects of CD53 expression on neutrophils appear to be related with recurrent infectious diseases. Cross-linking CD53 using CD53 antibodies led to cytoplasmic calcium fluxes in B cells, monocytes, and granulocytes and activation of the monocyte oxidative burst.
 Form/Format    200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
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 Cellular Localization    Cell Surface
 Immunogen    Human Sezary cells
 Hu-Chromosome Location    1p13
 Positive Control    Daudi, Raji, IM9, U266, YT, HUT-78, HUT-102, Jurkat, HL-60, THP-1, KG1a, human leukocytes. Human tonsil and lymph node.
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 Preparation and Storage    Antibody with azide - store at 2 to 8 degree C. Antibody without azide - store at -20 to -80 degree C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
 Other Notes    Small volumes of anti-CD53 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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 Applications Tested/Suitable for anti-CD53 antibody   

Functional Studies, Flow Cytometry (FC/FACS), Immunofluorescence (IF)

 Application Notes for anti-CD53 antibody    Functional Studies (Order Ab without Azide)
Flow Cytometry (0.5-1ug/million cells in 0.1ml)
Immunofluorescence (0.5-1ug/ml)
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NCBI/Uniprot data below describe general gene information for CD53. It may not necessarily be applicable to this product.
 NCBI GI #    10834972
 NCBI GeneID    963
 NCBI Accession #    NP_000551.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_000560.3 [Other Products]
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 UniProt Primary Accession #    P19397 [Other Products]
 UniProt Secondary Accession #    Q5U0D6; B2R905 [Other Products]
 UniProt Related Accession #    P19397 [Other Products]
 Molecular Weight    33-55kDa
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 NCBI Official Full Name    leukocyte surface antigen CD53 isoform 1
 NCBI Official Synonym Full Names    CD53 molecule
 NCBI Official Symbol    CD53 [Similar Products]
 NCBI Official Synonym Symbols   
[Similar Products]
 NCBI Protein Information    leukocyte surface antigen CD53
 UniProt Protein Name    Leukocyte surface antigen CD53
 UniProt Synonym Protein Names   
Cell surface glycoprotein CD53; Tetraspanin-25; Tspan-25; CD_antigen: CD53
 Protein Family    Leukocyte surface antigen
 UniProt Gene Name    CD53 [Similar Products]
 UniProt Synonym Gene Names    MOX44; TSPAN25; Tspan-25 [Similar Products]
 UniProt Entry Name    CD53_HUMAN
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 NCBI Summary for CD53    The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This encoded protein is a cell surface glycoprotein that is known to complex with integrins. It contributes to the transduction of CD2-generated signals in T cells and natural killer cells and has been suggested to play a role in growth regulation. Familial deficiency of this gene has been linked to an immunodeficiency associated with recurrent infectious diseases caused by bacteria, fungi and viruses. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2016]
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 UniProt Comments for CD53    CD53: May be involved in growth regulation in hematopoietic cells. Belongs to the tetraspanin (TM4SF) family.

Protein type: Membrane protein, integral; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 1p13

Cellular Component: immunological synapse; integral to plasma membrane; intercellular junction; plasma membrane

Molecular Function: protein binding

Biological Process: cell surface receptor linked signal transduction
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Product References and Citations for anti-CD53 antibody

   Knapp, W. et al., Leucocyte typing IV, p 534 and p 541. Oxford Univ. Press. 1989 Schlossman SF et al. eds. Leukocyte Typing V, p556-559, Oxford University Press, Oxford, 1995. Kishimoto T et al. eds. Leukocyte Typing VI, Garland Publishing, New York, 1997. Olweus J et al. CD53, a protein with four membrane-spanning domains, mediates signal transduction in human monocytes and B cells. J Immunol 1993, 151(2):707-716. Mannion BA et al. Transmembrane-4 superfamily proteins CD81 (TAPA-1), CD82, CD63, and CD53 specifically associated with integrin 4 beta 1 (CD49d/CD29). J Immunol 1996, 157(5):2039-2047. Carmo AM et al. Association of the transmembrane 4-superfamily molecule CD53 with a tyrosine phosphatase activity. Eur J Immunol 1995, 25(7):2090-2095. Mollinedo F et al. Recurrent infectious diseases in human CD53 deficiency. Clin Diagn Lab Immunol 1997, 4(2):229-231
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 Research Articles on CD53    1. CD53 is associated with population asthma risk via the functional promoter polymorphism -1560 C>T.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-CD53 antibodyDiseases associated with anti-CD53 antibody
 Reference Product  PubMed Publications
 CD9 antibody  >58 publications with CD53 and CD9
 CD81 antibody  >48 publications with CD53 and CD81
 CD82 antibody  >38 publications with CD53 and CD82
 CD4 antibody  >26 publications with CD53 and CD4
 CD2 antibody  >11 publications with CD53 and CD2
 Disease Name  Pubmed Publications
 Inflammation Antibodies  >9 publications with CD53 and Inflammation
 Necrosis Antibodies  >5 publications with CD53 and Necrosis
 Kidney Diseases Antibodies  >3 publications with CD53 and Kidney Diseases
 Atrophy Antibodies  >3 publications with CD53 and Atrophy
 Disease Models, Animal Antibodies  >2 publications with CD53 and Disease Models, Animal
 Heart Diseases Antibodies  >2 publications with CD53 and Heart Diseases
 Hypertension Antibodies  >1 publications with CD53 and Hypertension
 Breast Neoplasms Antibodies  >1 publications with CD53 and Breast Neoplasms
 Liver Diseases Antibodies  >1 publications with CD53 and Liver Diseases
 Fibrosis Antibodies  >1 publications with CD53 and Fibrosis
Organs/Tissues associated with anti-CD53 antibody
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >80 publications with CD53 and Blood
 Thymus Antibodies  >14 publications with CD53 and Thymus
 Vascular Antibodies  >9 publications with CD53 and Vascular
 Spleen Antibodies  >8 publications with CD53 and Spleen
 Bone Antibodies  >8 publications with CD53 and Bone
 Bone Marrow Antibodies  >7 publications with CD53 and Bone Marrow
 Lung Antibodies  >6 publications with CD53 and Lung
 Nerve Antibodies  >5 publications with CD53 and Nerve
 Kidney Antibodies  >5 publications with CD53 and Kidney
 Lymph Node Antibodies  >4 publications with CD53 and Lymph Node
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