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anti-CD73 antibody :: Mouse anti-Human CD73 Monoclonal Antibody

Scan QR to view Datasheet Catalog #    MBS200071
Unit / Price
0.05 mL  /  $240 +1 FREE 8GB USB
0.1 mL  /  $315 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

CD73, Monoclonal Antibody

★Popular Item★
 Also Known As   

CD73 antibody

 Product Synonym Names    5' nucleotidase; ecto; 5'-nucleotidase ecto (CD73); 5' nucleotidase (CD73); CD73; eN; eNT
 Product Gene Name   

anti-CD73 antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 OMIM    X55740 mRNA
 3D Structure    ModBase 3D Structure for P21589
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 Clonality    Monoclonal
 Isotype    IgG2b, k
 Clone Number    AT89E9
 Host    Mouse
 Species Reactivity    Human
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 Purity/Purification    By protein-G affinity chromatography
 Form/Format    Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
 Concentration    1mg/ml (lot specific)
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 Antigen Species    Human
 Immunogen    Recombinant human CD73 (27-252aa) purified from E. coli
 Clone Note    Anti-human CD73 mAb, clone AT89E9, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human CD73 protein.
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 Preparation and Storage    Can be stored at 4 degree C.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
 Other Notes    Small volumes of anti-CD73 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for anti-CD73 antibody

   CD73 (Ecto-5-nucleotidase, 5-NT) is a membrane-bound glycoprotein that functions to hydrolyze extracellular nucleoside monophosphates into bioactive nucleoside intermediates. This enables the uptake of adenosine, inosine, and guanosine into the cell and their subsequent reconversion into ATP and GTP in the purine salvage pathway. The expression of CD73 on neuronal cells has been described during developmental processes and, on lymphocytes, CD73 serves as a maturation marker, being absent from the surface of both immature B and T cells.
 Product Categories/Family for anti-CD73 antibody    Immunology
 Applications Tested/Suitable for anti-CD73 antibody   

ELISA (EIA), Western Blot (WB), Flow Cytometry (FC/FACS), Immunocytochemistry (ICC), Immunofluorescence (IF)

 Application Notes for anti-CD73 antibody    WB: 1:1,000 ~ 2,000.
Recommended starting dilution is 1:1,000.
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 Western Blot (WB) of anti-CD73 antibody    The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CD73 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: HepG2 cell lysate Lane 2.: WiDr cell lysate Lane 3.: A431 cell lysate
anti-CD73 antibody Western Blot (WB) (WB) image
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 Immunofluorescence (IF) of anti-CD73 antibody    ICC/IF analysis of CD73 in HeLa cells. The cell was stained with ACD0806 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
anti-CD73 antibody Immunofluorescence (IF) image
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NCBI/Uniprot data below describe general gene information for CD73. It may not necessarily be applicable to this product.
 NCBI GI #    4505467
 NCBI GeneID    4907
 NCBI Accession #    NP_002517 [Other Products]
 NCBI GenBank Nucleotide #    NM_002526.3 [Other Products]
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 UniProt Primary Accession #    P21589 [Other Products]
 UniProt Secondary Accession #    O75520; Q5W116; B3KQI8 [Other Products]
 UniProt Related Accession #    P21589 [Other Products]
 Molecular Weight    57,949 Da
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 NCBI Official Full Name    5'-nucleotidase isoform 1 preproprotein
 NCBI Official Synonym Full Names    5'-nucleotidase ecto
 NCBI Official Symbol    NT5E [Similar Products]
 NCBI Official Synonym Symbols   
NT; eN; NT5; NTE; eNT; CD73; E5NT; CALJA
[Similar Products]
 NCBI Protein Information    5'-nucleotidase
 UniProt Protein Name    5'-nucleotidase
 UniProt Synonym Protein Names   
Ecto-5'-nucleotidase; CD_antigen: CD73
 UniProt Gene Name    NT5E [Similar Products]
 UniProt Synonym Gene Names    NT5; NTE; 5'-NT [Similar Products]
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 NCBI Summary for CD73    The protein encoded by this gene is a plasma membrane protein that catalyzes the conversion of extracellular nucleotides to membrane-permeable nucleosides. The encoded protein is used as a determinant of lymphocyte differentiation. Defects in this gene can lead to the calcification of joints and arteries. Two transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Mar 2011]
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 UniProt Comments for CD73    Hydrolyzes extracellular nucleotides into membrane permeable nucleosides. Exhibits AMP-, NAD-, and NMN-nucleosidase activities.
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Product References and Citations for anti-CD73 antibody

   Sern P.C, et al., (2006) Purinergic Signal 2 (2):351-360; Linda F.T, et al., (2004) J Exp Med 200 (11):1395-1405; Laura A, et al., (1997) J Cell Biol 136 (2):421-431
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 Research Articles on CD73    1. Data show that MiR-422a and its target CD73 are involved in early loco(regional) recurrence of head and neck squamous cell carcinoma (HNSCC) tumors and are targets for personalized medicine.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-CD73 antibodyPathways associated with anti-CD73 antibody
 Reference Product  PubMed Publications
 ENPP1 antibody  >7 publications with CD73 and ENPP1
 ADA antibody  >7 publications with CD73 and ADA
 ENTPD1 antibody  >6 publications with CD73 and ENTPD1
 HIF1A antibody  >5 publications with CD73 and HIF1A
 CECR1 antibody  >1 publications with CD73 and CECR1
 Products by Pathway  Pathway Diagram
 Differentiation Pathway antibodies  Differentiation Pathway Diagram
 HIF-1-alpha Transcription Factor Network Pathway antibodies  HIF-1-alpha Transcription Factor Network Pathway Diagram
 Metabolic Pathways antibodies  Metabolic Pathways Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram
 Metabolism Of Nucleotides Pathway antibodies  Metabolism Of Nucleotides Pathway Diagram
 Metabolism Of Vitamins And Cofactors Pathway antibodies  Metabolism Of Vitamins And Cofactors Pathway Diagram
 Metabolism Of Water-soluble Vitamins And Cofactors Pathway antibodies  Metabolism Of Water-soluble Vitamins And Cofactors Pathway Diagram
 NAD+ Metabolism Pathway antibodies  NAD+ Metabolism Pathway Diagram
 Nicotinate And Nicotinamide Metabolism Pathway antibodies  Nicotinate And Nicotinamide Metabolism Pathway Diagram
 Nicotinate And Nicotinamide Metabolism Pathway antibodies  Nicotinate And Nicotinamide Metabolism Pathway Diagram
Diseases associated with anti-CD73 antibodyOrgans/Tissues associated with anti-CD73 antibody
 Disease Name  Pubmed Publications
 Inflammation Antibodies  >34 publications with CD73 and Inflammation
 Disease Models, Animal Antibodies  >21 publications with CD73 and Disease Models, Animal
 Breast Neoplasms Antibodies  >16 publications with CD73 and Breast Neoplasms
 Heart Diseases Antibodies  >12 publications with CD73 and Heart Diseases
 Nervous System Diseases Antibodies  >11 publications with CD73 and Nervous System Diseases
 Necrosis Antibodies  >10 publications with CD73 and Necrosis
 Neoplasms, Experimental Antibodies  >8 publications with CD73 and Neoplasms, Experimental
 Kidney Diseases Antibodies  >6 publications with CD73 and Kidney Diseases
 Liver Neoplasms Antibodies  >6 publications with CD73 and Liver Neoplasms
 Liver Diseases Antibodies  >6 publications with CD73 and Liver Diseases
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >67 publications with CD73 and Blood
 Vascular Antibodies  >41 publications with CD73 and Vascular
 Bone Antibodies  >26 publications with CD73 and Bone
 Brain Antibodies  >19 publications with CD73 and Brain
 Bone Marrow Antibodies  >17 publications with CD73 and Bone Marrow
 Lung Antibodies  >17 publications with CD73 and Lung
 Heart Antibodies  >15 publications with CD73 and Heart
 Mouth Antibodies  >14 publications with CD73 and Mouth
 Muscle Antibodies  >13 publications with CD73 and Muscle
 Connective Tissue Antibodies  >12 publications with CD73 and Connective Tissue
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