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anti-CD8 antibody :: Rat CD8 ALPHA Monoclonal Antibody

Scan QR to view Datasheet Catalog #    MBS214268
Unit / Price
0.5 mg  /  $540 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

CD8 ALPHA, Monoclonal Antibody

 Also Known As   

RAT ANTI MOUSE CD8:Low Endotoxin

 Product Gene Name   

anti-CD8 antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Clonality    Monoclonal
 Isotype    IgG2a
 Clone Number    YTS105.18
 Host    Rat
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 Form/Format    Low Endotoxin
Purified IgG - liquid
 Concentration    IgG concentration 1 mg/ml (lot specific)
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 Preparation    Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
 Buffer Solution    Phosphate buffered saline
 Target Species    Mouse
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 Preparation and Storage    Store at -20 degree C only. This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.
 Other Notes    Small volumes of anti-CD8 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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 Applications Tested/Suitable for anti-CD8 antibody   

Immunohistology Frozen, Flow cytometry (FC/FACS), Immunofluorescence (IF)

 Application Notes for anti-CD8 antibody    Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry: Minimum Dilution: 1/50; Maximum Dilution: 1/100
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 Testing Data of anti-CD8 antibody    Published customer image: NKG2D-dependent infiltration of CD8+T cells into tumors. Tumors were collected from mice that had received one of the four standard treatments: control DNA, Dox plus control DNA, IL-12, Dox plus IL-12 (n = 3 per treatment group). (A) Infiltration of NKG2D-positive cells into tumors. Northern blot analysis was performed to detect NKG2D expression in tumors. Ribosomal RNA was used to confirm equal loading among samples. (B) NKG2D/CD8 -positive cells in tumor sections by treatment received. Frozen tumor sections were stained with biotin anti-mouse NKG2D, anti-mouse CD8, or corresponding isotype control antibodies, then with streptavidin-conjugated Alexa fluor 594 or Alexa fluor 488 secondary antibodies. Data shown are representative of three independent experiments. The scale bar is equivalent to 100 um.From: CD8+T cell-specific induction of NKG2D receptor by doxorubicin plus interleukin-12 and its contribution to CD8+T cell accumulation in tumors. Hu J, Zhu S, Xia X, Zhang L, Kleinerman ES, Li S. Mol Cancer. 2014 Feb 24;13:34.
anti-CD8 antibody Testing Data image
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 Testing Data of anti-CD8 antibody    Immunofluorescence staining of a mouse lymph node cryosection with Rat anti mouse CD19, clone 6D5 (MBS212149), green in A and Rat anti Mouse CD8 , red in B. Merged image in C wih nuclei counterstained blue using DAPI. Low power
anti-CD8 antibody Testing Data image
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 Testing Data of anti-CD8 antibody    Staining of mouse spleen cells with Rat anti Mouse CD8 (MBS212026)
anti-CD8 antibody Testing Data image
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 Testing Data of anti-CD8 antibody    Immunoperoxidase staining of Mouse lymph node cryosection using Rat anti Mouse CD8alpha antibody followed by horseradish peroxidase conjugated Goat anti Rat IgG (MBS235195) for detection. Low power
anti-CD8 antibody Testing Data image
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 Testing Data of anti-CD8 antibody    Immunofluorescence staining of mouse lymph node cryosection using Rat anti Mouse CD11b antibody , green in A and Rat anti Mouse CD8 antibody , red in B, Merged image in C
anti-CD8 antibody Testing Data image
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 Testing Data of anti-CD8 antibody    Published customer image: Recruitment of inflammatory cells in the intestine of neonates after CpG-ODN treatment. Eight-day-old neonatal mice received 20 ug/g of CpG-ODN orally. Twenty-four hours after treatment, ilea were removed to analyze the recruitment of inflammatory cells by immunohistology. Six to ten neonates from different litters (5 sections for each animal) per group were analyzed. Data were analyzed by non-parametric Mann-Whitney test.From: Lacroix-Lamand© S, Rochereau N, Mancassola R, Barrier M, Clauzon A, et al. (2009) Neonate Intestinal Immune Response to CpG Oligodeoxynucleotide Stimulation. PLoS ONE 4(12): e8291.
anti-CD8 antibody Testing Data image
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 Testing Data of anti-CD8 antibody    immunofluorescence staining of mouse lymph node cryosection with Rat anti Mouse Ly-6B.2 antibody, clone 7/4 (MBS216530), green in A and Rat anti Mouse CD8 antibody, clone YTS105.18 , red in B. C is the merged image with nuclei counterstained blue using DAPI. High power
anti-CD8 antibody Testing Data image
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 Testing Data of anti-CD8 antibody    Published customer image: The effect of NKG2D-blocking antibody on NKG2D-positive CD8+T cells and CD8+T cell accumulation in tumors. 4T-1 tumor -bearing mice were subjected to one of two treatments: Dox plus IL-12 plus control IgG or Dox plus IL-12 plus NKG2D-blocking antibody (n = 3 per treatment). (A) NKG2D expression in CD8+T cells was determined as described for Figure 1 (n.s., not significant). (B) The presence of NKG2D/CD8 -positive cells in tumor sections after the indicated treatments were determined as described for Figure 3.From: CD8+T cell-specific induction of NKG2D receptor by doxorubicin plus interleukin-12 and its contribution to CD8+T cell accumulation in tumors. Hu J, Zhu S, Xia X, Zhang L, Kleinerman ES, Li S. Mol Cancer. 2014 Feb 24;13:34.
anti-CD8 antibody Testing Data image
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 Testing Data of anti-CD8 antibody    Immunofluorescence staining of mouse lymph node cryosection with Rat anti Mouse CD11b, clone 5C6 (MBS216530), green in A and Rat anti Mouse CD8, clone YTS105.18 , red in B. C is the merged image with nuclei counterstained blue using DAPI. Low power
anti-CD8 antibody Testing Data image
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 Testing Data of anti-CD8 antibody    Immunofluorescence staining of mouse lymph node cryosection with Rat anti Mouse CD11b, clone 5C6 (MBS216530), green in A and Rat anti Mouse CD8, clone YTS105.18 , red in B. C is the merged image with nuclei counterstained blue using DAPI. High power
anti-CD8 antibody Testing Data image
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NCBI/Uniprot data below describe general gene information for CD8. It may not necessarily be applicable to this product.
 NCBI GI #    124487437
 NCBI GeneID    12525
 NCBI Accession #    NP_001074579.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001081110.2 [Other Products]
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 UniProt Related Accession #    P01731 [Other Products]
 Molecular Weight    27,456 Da
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 NCBI Official Full Name    T-cell surface glycoprotein CD8 alpha chain isoform 1
 NCBI Official Synonym Full Names    CD8 antigen, alpha chain
 NCBI Official Symbol    Cd8a [Similar Products]
 NCBI Official Synonym Symbols   
Ly-2; Ly-B; Ly-35; Lyt-2; BB154331
[Similar Products]
 NCBI Protein Information    T-cell surface glycoprotein CD8 alpha chain; Lyt-2.1 lymphocyte differentiation antigen (AA at 100); T-cell surface glycoprotein Lyt-2
 UniProt Protein Name    T-cell surface glycoprotein CD8 alpha chain
 UniProt Synonym Protein Names   
T-cell surface glycoprotein Lyt-2; CD_antigen: CD8a
 UniProt Gene Name    Cd8a [Similar Products]
 UniProt Synonym Gene Names    Lyt-2; Lyt2 [Similar Products]
 UniProt Entry Name    CD8A_MOUSE
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 UniProt Comments for CD8    CD8A: Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains. In general heterodimer of an alpha and a beta chain linked by two disulfide bonds. Can also form homodimers. Shown to be expressed as heterodimer on thymocytes and as homodimer on peripheral blood T-lymphocytes. Interacts with the MHC class I HLA-A/B2M dimer. Interacts with LCK in a zinc-dependent manner. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Cellular Component: cell surface; membrane; integral to membrane; external side of plasma membrane

Molecular Function: protein binding; protein homodimerization activity; protein kinase binding

Biological Process: cell surface receptor linked signal transduction; T cell activation; immune system process; T cell mediated immunity; positive regulation of calcium-mediated signaling; cytotoxic T cell differentiation; defense response to virus
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 Research Articles on CD8    1. CD8alpha DCs, rather than CD8 T cells, are responsible for enhanced viral latency and recurrences.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with anti-CD8 antibodyDiseases associated with anti-CD8 antibody
 Products by Pathway  Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 Antigen Processing And Presentation Pathway antibodies  Antigen Processing And Presentation Pathway Diagram
 Antigen Processing And Presentation Pathway antibodies  Antigen Processing And Presentation Pathway Diagram
 Cell Adhesion Molecules (CAMs) Pathway antibodies  Cell Adhesion Molecules (CAMs) Pathway Diagram
 Cell Adhesion Molecules (CAMs) Pathway antibodies  Cell Adhesion Molecules (CAMs) Pathway Diagram
 Hematopoietic Cell Lineage Pathway antibodies  Hematopoietic Cell Lineage Pathway Diagram
 Hematopoietic Cell Lineage Pathway antibodies  Hematopoietic Cell Lineage Pathway Diagram
 Immune System Pathway antibodies  Immune System Pathway Diagram
 Immunoregulatory Interactions Between A Lymphoid And A Non-Lymphoid Cell Pathway antibodies  Immunoregulatory Interactions Between A Lymphoid And A Non-Lymphoid Cell Pathway Diagram
 Primary Immunodeficiency Pathway antibodies  Primary Immunodeficiency Pathway Diagram
 Disease Name  Pubmed Publications
 Inflammation Antibodies  >19 publications with CD8 and Inflammation
 Necrosis Antibodies  >12 publications with CD8 and Necrosis
 Cardiovascular Diseases Antibodies  >9 publications with CD8 and Cardiovascular Diseases
 Neoplasms, Experimental Antibodies  >8 publications with CD8 and Neoplasms, Experimental
 Carcinoma Antibodies  >7 publications with CD8 and Carcinoma
 Liver Diseases Antibodies  >7 publications with CD8 and Liver Diseases
 Kidney Diseases Antibodies  >5 publications with CD8 and Kidney Diseases
 Fibrosis Antibodies  >4 publications with CD8 and Fibrosis
 Liver Neoplasms Antibodies  >3 publications with CD8 and Liver Neoplasms
 Heart Diseases Antibodies  >3 publications with CD8 and Heart Diseases
Organs/Tissues associated with anti-CD8 antibody
 Organ/Tissue Name  Pubmed Publications
 Spleen Antibodies  >26 publications with CD8 and Spleen
 Thymus Antibodies  >15 publications with CD8 and Thymus
 Bone Marrow Antibodies  >12 publications with CD8 and Bone Marrow
 Thyroid Antibodies  >3 publications with CD8 and Thyroid
 Embryonic Tissue Antibodies  >2 publications with CD8 and Embryonic Tissue
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