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anti-CD8 antibody :: Rabbit anti-Human CD8 Monoclonal Antibody

Scan QR to view Datasheet Catalog #    MBS684124
Immunohistochemistry (IHC)
Unit / Price
1 ml (Prediluted)  /  $175 +1 FREE 8GB USB
0.04 mL  /  $185 +1 FREE 8GB USB
0.1 mL  /  $360 +1 FREE 8GB USB
7 ml (Prediluted)  /  $390 +1 FREE 8GB USB
0.2 mL  /  $490 +1 FREE 8GB USB
15 ml (Prediluted)  /  $570 +1 FREE 8GB USB
0.5 mL  /  $615 +1 FREE 8GB USB
1 mL  /  $955 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

CD8, Monoclonal Antibody

★Popular Item★
 Also Known As   

Anti - CD8

 Product Gene Name   

anti-CD8 antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Chromosome Location    Chromosome: 2; NC_000002.12 (86784605..86808396, complement). Location: 2p12
 OMIM    186910
 3D Structure    ModBase 3D Structure for P01732
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 Clonality    Monoclonal
 Clone Number    P17-V
 Host    Rabbit
 Species Reactivity    Human
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 Specificity    Human
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 Immunogen    Peptide derived from C-terminal region of human CD8
 Storage Buffer    20 mM Tris-HCI, pH 8.0
 Stabilizer    20 mg/ml BSA
 Preservative    0.05% NaN3
 Immunogen    Peptide derived from C-terminal region of human CD8. Antibody recognizes the epitope between Pro218 - Tyr234.
 Positive control    human tonsil tissue
  Cellular localization    membrane
 IHC-P PROTOCOL–INSTRUCTION MANUAL    1.Deparaffinize the section in 3 changes of xylene, 5 minutes each.
2.Wash the section in 96%, 80% and 70% ethyl alcohol for 10 minutes each.
3.Rinse in distilled water.
4.Block the endogenous peroxidase by incubating the tissue in 3% hydrogen peroxide (H2O2) for 10 minutes.
5.Wash in distilled water.
6.For antigen retrieval: immerse the slide in Tris-EDTA buffer, pH 9.0,0.05% Tween-20*, and incubatein water bath at 96-98ºC for 20-25 minutes.
7.Transfer the slide to room temperature and let it cool down for 15 minutes in the buffer used in the step #6
8.Rinse in distilled water.
9.Wash in 0.05 M Tris-HCl, pH 7.6 buffer supplemented with 0.2% of Tween-20 (buffer A) for 5 minutes.
10.READY TO USE (RTU): Incubate the section with primary antibody (ready to use)for 1 hour in a closed wet chamber.
11.Wash twice 5 minutes with buffer A.
12.Apply the secondary antibody (the protocol depends on the supplier), and proceed to standard mmunohistochemistry protocol .
13.Wash twice 5 minutes with buffer A.
14.Apply the chromogen (DAB), 1 -3 minutes.
15.Wash in water –10 minutes.
16.Stain in hematoxylin for 5 minutes.
17.Wash in water –10 minutes.8.Dehydrate the section in 2 changes of 96% ethyl alcohol for 5 minutes each.
19.Wash the section in 2 changes of xylene for 2 minutes each.
20.Mount the slide for observation.
 PRECAUTIONS    1.We strongly recommend to use DB Primary Antibody Diluent eventually alternative diluent (containing protease free BSA at the concentrations ? 1mg/ml) for dilution of concentrated antibodies, otherwise the warranty might be oided.
2.Centrifuge the vial before use.3.Intended for professional In Vitro Diagnostic use in laboratories.
4.Do not use after expiration date stamped on vial label.
5.Avoid contamination of the reagent.
6.Any discrepancies in the recommended procedures stated in the working protocol may affect the final results.
7.The reagent contains sodium azide (NaN3) which is highly toxic in higher concentrations. The concentration in the reagent (0.05%) is not considered as hazardous.
8.Disposal of waste material must be conducted in accordance with local regulations.
9.Wear appropriate Personal Protective Equipment to avoid contact with eyes and skin.
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 Preparation and Storage    Store at +4°C. Do not freeze!
 ISO Certification    Manufactured in an ISO 9001:2008 and ISO 13485:2003 Certified Laboratory.
 Other Notes    Small volumes of anti-CD8 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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 Applications Tested/Suitable for anti-CD8 antibody   

Immunohistochemistry (IHC) - Formalin/Paraffin

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 Immunohistochemistry (IHC) of anti-CD8 antibody    CD8 expression in T-lymphocytes of the palatine tonsil. Formalin fixed, paraffin embedded human tissue (4 ?msection) stained with anti -CD8 (MBS684124) monospecific clonal antibody
anti-CD8 antibody Immunohistochemistry (IHC) (IHC) image
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NCBI/Uniprot data below describe general gene information for CD8. It may not necessarily be applicable to this product.
 NCBI GI #    116035
 NCBI GeneID    925
 NCBI Accession #    P01732.1 [Other Products]
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 UniProt Primary Accession #    P01732 [Other Products]
 UniProt Secondary Accession #    Q13970; Q4ZG17; D6W5M8 [Other Products]
 UniProt Related Accession #    P01732 [Other Products]
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 NCBI Official Full Name    T-cell surface glycoprotein CD8 alpha chain
 NCBI Official Synonym Full Names    CD8a molecule
 NCBI Official Symbol    CD8A [Similar Products]
 NCBI Official Synonym Symbols   
CD8; MAL; p32; Leu2
[Similar Products]
 NCBI Protein Information    T-cell surface glycoprotein CD8 alpha chain; T8 T-cell antigen; T cell co-receptor; OKT8 T-cell antigen; T-cell antigen Leu2; Leu2 T-lymphocyte antigen; CD8 antigen, alpha polypeptide (p32); T-lymphocyte differentiation antigen T8/Leu-2
 UniProt Protein Name    T-cell surface glycoprotein CD8 alpha chain
 UniProt Synonym Protein Names   
T-lymphocyte differentiation antigen T8/Leu-2
 Protein Family    CD81 antigen
 UniProt Gene Name    CD8A [Similar Products]
 UniProt Synonym Gene Names    MAL [Similar Products]
 UniProt Entry Name    CD8A_HUMAN
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 NCBI Summary for CD8    The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen acts as a coreceptor with the T-cell receptor on the T lymphocyte to recognize antigens displayed by an antigen presenting cell in the context of class I MHC molecules. The coreceptor functions as either a homodimer composed of two alpha chains or as a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. This gene encodes the CD8 alpha chain. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2011]
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 UniProt Comments for CD8    Function: Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains.

Subunit structure: In general heterodimer of an alpha and a beta chain linked by two disulfide bonds. Can also form homodimers. Shown to be expressed as heterodimer on thymocytes and as homodimer on peripheral blood T-lymphocytes. Interacts with the MHC class I HLA-A/B2M dimer. Interacts with LCK in a zinc-dependent manner. Ref.9

Subcellular location: Isoform 1: Cell membrane; Single-pass type I membrane protein. Isoform 2: Secreted.

Post-translational modification: All of the five most C-terminal cysteines form inter-chain disulfide bonds in dimers and higher multimers, while the four N-terminal cysteines do not

By similarity.

Involvement in disease: CD8 deficiency, familial (CD8 deficiency) [MIM:608957]: An immunologic defect characterized by absence of CD8+ cells, leading to recurrent bacterial infections.Note: The disease is caused by mutations affecting the gene represented in this entry.

Sequence similarities: Contains 1 Ig-like V-type (immunoglobulin-like) domain.
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 Research Articles on CD8    1. transcription factor cAMP-responsive element modulator alpha (CREMalpha), which is expressed at increased levels in T cells from systemic lupus erythematosus patients, contributes to transcriptional silencing of CD8A and CD8B.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-CD8 antibodyPathways associated with anti-CD8 antibody
 Reference Product  PubMed Publications
 CD4 antibody  >72 publications with CD8 and CD4
 CD8B antibody  >18 publications with CD8 and CD8B
 CD3E antibody  >4 publications with CD8 and CD3E
 CD3D antibody  >2 publications with CD8 and CD3D
 HLA-A antibody  >2 publications with CD8 and HLA-A
 HLA-B antibody  >1 publications with CD8 and HLA-B
 LCK antibody  >1 publications with CD8 and LCK
 Products by Pathway  Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 Antigen Processing And Presentation Pathway antibodies  Antigen Processing And Presentation Pathway Diagram
 Antigen Processing And Presentation Pathway antibodies  Antigen Processing And Presentation Pathway Diagram
 Cell Adhesion Molecules (CAMs) Pathway antibodies  Cell Adhesion Molecules (CAMs) Pathway Diagram
 Cell Adhesion Molecules (CAMs) Pathway antibodies  Cell Adhesion Molecules (CAMs) Pathway Diagram
 Downstream Signaling In Naive CD8+ T Cells Pathway antibodies  Downstream Signaling In Naive CD8+ T Cells Pathway Diagram
 Hematopoietic Cell Lineage Pathway antibodies  Hematopoietic Cell Lineage Pathway Diagram
 Hematopoietic Cell Lineage Pathway antibodies  Hematopoietic Cell Lineage Pathway Diagram
 IL12-mediated Signaling Events Pathway antibodies  IL12-mediated Signaling Events Pathway Diagram
 Immune System Pathway antibodies  Immune System Pathway Diagram
Diseases associated with anti-CD8 antibodyOrgans/Tissues associated with anti-CD8 antibody
 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >20 publications with CD8 and Neoplasms
 Inflammation Antibodies  >18 publications with CD8 and Inflammation
 Necrosis Antibodies  >12 publications with CD8 and Necrosis
 Neoplasms, Experimental Antibodies  >8 publications with CD8 and Neoplasms, Experimental
 Carcinoma Antibodies  >6 publications with CD8 and Carcinoma
 Liver Diseases Antibodies  >6 publications with CD8 and Liver Diseases
 Kidney Diseases Antibodies  >5 publications with CD8 and Kidney Diseases
 Fibrosis Antibodies  >4 publications with CD8 and Fibrosis
 Cerebral Infarction Antibodies  >3 publications with CD8 and Cerebral Infarction
 Hemorrhage Antibodies  >3 publications with CD8 and Hemorrhage
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >90 publications with CD8 and Blood
 Spleen Antibodies  >20 publications with CD8 and Spleen
 Liver Antibodies  >13 publications with CD8 and Liver
 Thymus Antibodies  >12 publications with CD8 and Thymus
 Kidney Antibodies  >11 publications with CD8 and Kidney
 Brain Antibodies  >10 publications with CD8 and Brain
 Intestine Antibodies  >8 publications with CD8 and Intestine
 Lung Antibodies  >5 publications with CD8 and Lung
 Connective Tissue Antibodies  >2 publications with CD8 and Connective Tissue
 Stomach Antibodies  >2 publications with CD8 and Stomach
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