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anti-CD8a antibody :: Mouse anti-Rat CD8a Monoclonal Antibody

Scan QR to view Datasheet Catalog #    MBS520223
Testing Data
Unit / Price
0.1 mg  /  $250 +1 FREE 8GB USB
0.5 mg  /  $420 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

CD8a, Monoclonal Antibody

 Also Known As   

Anti-Rat CD8a Biotin (Clone OX-8) (mouse IgG1)

 Product Synonym Names    CD8a Biotin (Clone OX-8) (mouse IgG1); Biotin Anti-Rat CD8a Monoclonal Antibody
 Product Gene Name   

anti-CD8a antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Chromosome Location    Chromosome: 2; NC_000002.11 (87011728..87035519, complement). Location: 2p12
 OMIM    186910
 3D Structure    ModBase 3D Structure for P01732
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 Clonality    Monoclonal
 Isotype    Mouse IgG1
 Clone Number    OX-8
 Host    Mouse
 Species Reactivity    Rat
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 Specificity    CD8a (Rat CD8a)
 Form/Format    Biotin (Biotin conjugated Ig buffered in PBS, 0.02% NaN3 and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/ml. (Purified from ascitic fluid via Protein G Chromatography))
 Concentration    Antibody Concentration: 0.1 mg/ml (lot specific)
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 Immunogen    High molecular weight rat thymocyte glycoproteins
 Donor    BALB/c spleen
 Fusion Partner    P3-NSI-1-Ag4 (NSI/1)
 Presentation    100 ug or 500 ug Biotin conjugated Ig buffered in PBS, 0.02% NaN3 and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/ml.
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 Preparation and Storage    Store at 4 degree C. For long term storage, aliquot and freeze unused portion at -20 degree C in volumes appropriate for single usage. Avoid freeze/thaw cycles.
 Other Notes    Small volumes of anti-CD8a antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for anti-CD8a antibody

   anti-rat CD8a monoclonal antibody recognizes a determinant on the majority of thymocytes (90-95%), a subset of peripheral T cells, the majority of NK cells, and the granular intraepithelial leukocytes in the small intestine. (1,2,3,4.) The antigen recognized is a complex of surface glycoproteins of Mr 34,000, 39,000 and 76,000 Da and is the rat homologue of the human CD8 and the mouse Ly-2,3 antigen. (5,8) MBS520223 labels all peripheral T cells that are unlabelled by the (W3/25) monoclonal antibody. It labels a T-cell subset which mediates the suppression of antibody formation (1) and the cytotoxic cell precursor. (2) MBS520223 can be used together to fractionate T cells by sorting in FACS or by rosette depletion (1,2) or can be used together to study subsets of T cells in the rat which mediate lethal graft vs. host disease. (7) This antibody is one of the 3 monoclonal antibodies which labels T lymphocyte populations in the rat, these being (W3/13) which labels all T cells, as well as (W3/25) and (MRC 0X-8) which label non-overlapping T cell populations. These monoclonal antibodies used in concert are being employed extensively to investigate cellular aspects of the immune response in rats and prove to be useful as markers for functionally distinct subpopulations of lymphocytes. This clone is reported to work with frozen and paraffin sections (9).
 Applications Tested/Suitable for anti-CD8a antibody   

Immunohistochemistry (IHC), Western Blot (WB), Flow Cytometry (FC/FACS)

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 Testing Data of anti-CD8a antibody    anti-CD8a antibody Testing Data image
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NCBI/Uniprot data below describe general gene information for CD8a. It may not necessarily be applicable to this product.
 NCBI GI #    19344022
 NCBI GeneID    925
 NCBI Accession #    AAH25715.1 [Other Products]
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 UniProt Primary Accession #    P01732 [Other Products]
 UniProt Secondary Accession #    Q13970; Q4ZG17; D6W5M8 [Other Products]
 UniProt Related Accession #    P01732 [Other Products]
 Molecular Weight    25,729 Da
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 NCBI Official Full Name    CD8a molecule
 NCBI Official Synonym Full Names    CD8a molecule
 NCBI Official Symbol    CD8A [Similar Products]
 NCBI Official Synonym Symbols   
CD8; MAL; p32; Leu2
[Similar Products]
 NCBI Protein Information    T-cell surface glycoprotein CD8 alpha chain; T8 T-cell antigen; T cell co-receptor; OKT8 T-cell antigen; T-cell antigen Leu2; Leu2 T-lymphocyte antigen; CD8 antigen, alpha polypeptide (p32); T-lymphocyte differentiation antigen T8/Leu-2
 UniProt Protein Name    T-cell surface glycoprotein CD8 alpha chain
 UniProt Synonym Protein Names   
T-lymphocyte differentiation antigen T8/Leu-2
 Protein Family    T-cell surface glycoprotein
 UniProt Gene Name    CD8A [Similar Products]
 UniProt Synonym Gene Names    MAL [Similar Products]
 UniProt Entry Name    CD8A_HUMAN
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 NCBI Summary for CD8a    The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen acts as a coreceptor with the T-cell receptor on the T lymphocyte to recognize antigens displayed by an antigen presenting cell in the context of class I MHC molecules. The coreceptor functions as either a homodimer composed of two alpha chains or as a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. This gene encodes the CD8 alpha chain. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2011]
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 UniProt Comments for CD8a    Function: Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains.

Subunit structure: In general heterodimer of an alpha and a beta chain linked by two disulfide bonds. Can also form homodimers. Shown to be expressed as heterodimer on thymocytes and as homodimer on peripheral blood T-lymphocytes. Interacts with the MHC class I HLA-A/B2M dimer. Interacts with LCK in a zinc-dependent manner. Ref.9

Subcellular location: Isoform 1: Cell membrane; Single-pass type I membrane protein. Isoform 2: Secreted.

Post-translational modification: All of the five most C-terminal cysteines form inter-chain disulfide bonds in dimers and higher multimers, while the four N-terminal cysteines do not

By similarity.

Involvement in disease: Familial CD8 deficiency (CD8 deficiency) [MIM:608957]: Novel autosomal recessive immunologic defect characterized by absence of CD8+ cells, leading to recurrent bacterial infections.Note: The disease is caused by mutations affecting the gene represented in this entry.

Sequence similarities: Contains 1 Ig-like V-type (immunoglobulin-like) domain.
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 Research Articles on CD8a    1. The CD8(+)CCR7(+) T-cell frequency in HNSCC patients' blood tested at diagnosis can discriminate them from normal controls and predicts disease recurrence
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with anti-CD8a antibodyDiseases associated with anti-CD8a antibody
 Products by Pathway  Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 Antigen Processing And Presentation Pathway antibodies  Antigen Processing And Presentation Pathway Diagram
 Antigen Processing And Presentation Pathway antibodies  Antigen Processing And Presentation Pathway Diagram
 Cell Adhesion Molecules (CAMs) Pathway antibodies  Cell Adhesion Molecules (CAMs) Pathway Diagram
 Cell Adhesion Molecules (CAMs) Pathway antibodies  Cell Adhesion Molecules (CAMs) Pathway Diagram
 Downstream Signaling In Naive CD8+ T Cells Pathway antibodies  Downstream Signaling In Naive CD8+ T Cells Pathway Diagram
 Hematopoietic Cell Lineage Pathway antibodies  Hematopoietic Cell Lineage Pathway Diagram
 Hematopoietic Cell Lineage Pathway antibodies  Hematopoietic Cell Lineage Pathway Diagram
 IL12-mediated Signaling Events Pathway antibodies  IL12-mediated Signaling Events Pathway Diagram
 Immune System Pathway antibodies  Immune System Pathway Diagram
 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >19 publications with CD8a and Neoplasms
 Inflammation Antibodies  >16 publications with CD8a and Inflammation
 Necrosis Antibodies  >12 publications with CD8a and Necrosis
 Cardiovascular Diseases Antibodies  >8 publications with CD8a and Cardiovascular Diseases
 Neoplasms, Experimental Antibodies  >7 publications with CD8a and Neoplasms, Experimental
 Carcinoma Antibodies  >6 publications with CD8a and Carcinoma
 Kidney Diseases Antibodies  >5 publications with CD8a and Kidney Diseases
 Liver Diseases Antibodies  >5 publications with CD8a and Liver Diseases
 Lymphoma Antibodies  >4 publications with CD8a and Lymphoma
 Cerebral Infarction Antibodies  >3 publications with CD8a and Cerebral Infarction
Organs/Tissues associated with anti-CD8a antibody
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >87 publications with CD8a and Blood
 Spleen Antibodies  >19 publications with CD8a and Spleen
 Liver Antibodies  >12 publications with CD8a and Liver
 Thymus Antibodies  >11 publications with CD8a and Thymus
 Brain Antibodies  >10 publications with CD8a and Brain
 Kidney Antibodies  >10 publications with CD8a and Kidney
 Intestine Antibodies  >7 publications with CD8a and Intestine
 Lung Antibodies  >5 publications with CD8a and Lung
 Connective Tissue Antibodies  >2 publications with CD8a and Connective Tissue
 Stomach Antibodies  >2 publications with CD8a and Stomach
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