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anti-CD90 (Thy 1.1) antibody :: Mouse anti-Mouse CD90 (Thy 1.1) Monoclonal Antibody

Scan QR to view Datasheet Catalog #    MBS520243
Testing Data
Unit / Price
5 mL  /  $355 +1 FREE 8GB USB
 
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 Product Name   

CD90 (Thy 1.1) (CD90 (Thy 1.1)), Monoclonal Antibody

★Popular Item★
 Also Known As   

Anti-Mouse CD90 (Thy 1.1), Supernatant (Clone T11D7e) (mouse IgM)

 Product Synonym Names    CD90 (Thy 1.1), Supernatant (Clone T11D7e) (mouse IgM); Anti-Mouse CD90 (Thy 1.1) Monoclonal Antibody - Supernatant
 Product Gene Name   

anti-CD90 (Thy 1.1) antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Clonality    Monoclonal
 Isotype    Mouse IgM
 Clone Number    T11D7e
 Host    Mouse
 Species Reactivity    Mouse
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 Specificity    Mouse CD90 (Thy 1.1)
 Form/Format    Supernatant (Lyophilized.)
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 Immunogen    Rat Thymocytes
 Donor    CBA spleen
 Fusion Partner    myeloma P3-NSI-1-AG 4-1
 Presentation    5 ml lyophilized
 Tissue Distribution by Cytotoxicity Analysis    Antibody Concentration Used: 1:320
Strain: AKR/J
 Cell Source = C.I.    Thymus: 98
Spleen: 18
Lymph Node: 33
Bone Marrow: 6
 Strain Distribution by Cytotoxicity Analysis    Antibody Concentration Used: 1:20
Strains Tested: AKR/J, C57BL/6, BALB/c, C3H/He, A.TH
Positive: AKR/J
Negative: C57BL/6, BALB/c, C3H/He, A.TH
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 Preparation and Storage    Store at -20 degree C. For long term storage, aliquot and freeze unused portion at -20 degree C in volumes appropriate for single usage. Avoid freeze/thaw cycles.
Shipping: Ships at 4°C
 Other Notes    Small volumes of anti-CD90 (Thy 1.1) antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for anti-CD90 (Thy 1.1) antibody

   Anti-mouse CD90 (Thy 1.1) monoclonal antibody is specific for T cells from mouse strains expressing the Thy 1.1 phenotype (eg. AKR, RF). MBS520243 can be used to selectively identify or deplete T cells or their precursors. Cross reacts with rat Thy 1.
 Applications Tested/Suitable for anti-CD90 (Thy 1.1) antibody   

Cytotoxic Assay (CA)

 Application Notes for anti-CD90 (Thy 1.1) antibody    CYTOTOXICITY ANALYSIS: Method:
1. Prepare a cell suspension from the appropriate tissue in Cytotoxicity Mediuma or equivalent. Remove red cells and dead cells (where necessary) by purification of viable lymphocytes on Lympholyte®-Mb density cell separation medium. After washing, adjust the cell concentration to 1x106 cells per ml in Cytotoxicity Medium.
2. Add the antibody to a final concentration of 1:1000 and mix.
3. Incubate for 60 minutes at 4°C.
4. Centrifuge to pellet the cells and discard the supernatant.
5. Resuspend to the original volume in Low-Tox®-M Rabbit Complementc diluted to the recommended concentration in Cytotoxicity Medium.
6. Incubate for 60 minutes at 37°C.
7. Place on ice.
8. Add Trypan Blue, 10% by volume of 1% Trypan Blue (w/v) added 3-5 minutes before scoring works well.
Score live versus dead cells in a hemacytometer.
Cytotoxic Index (C. I.) can be calculated as follows:
C.I. = 100 x % cyt (antibody + complement) - % cyt (complement alone) *over* 100% - % cyt (complement alone)

CYTOTOXICITY DEPLETION ASSAY: Method:
1. Prepare a cell suspension from the appropriate tissue in Cytotoxicity Medium or equivalent. Remove red cells and dead cells (where necessary) by purification of viable lymphocytes on Lympholyte®-M cell separation medium. After washing, adjust the cell concentration to 1x107 cells per ml in Cytotoxicity Medium.
2. Add the antibody to a final concentration of 1:20 and mix. Alternatively, pellet the cells and resuspend in antibody diluted 1:20 in Cytotoxicity Medium.
3. Incubate for 60 minutes at 4°C.
4. Centrifuge to pellet the cells and discard the supernatant.
5. Resuspend to the original volume in Low-Tox-M® Rabbit Complement, diluted to the appropriate concentration in Cytotoxicity Medium. (Recommended concentration included with each batch of Low-Tox-M® Rabbit Complement.)
6. Incubate for 60 minutes at 37°C.
7. Monitor for percent cytotoxicity at this stage, before further processing. For this purpose, remove a small sample from each tube, dilute 1:10 with medium, and add 1/10 volume of 1% Trypan Blue. After 3-5 minutes, score live versus dead cells in a hemacytometer.
8. For functional studies, remove the dead cells from the treated groups before further processing, particularly if the treated cells are to be cultured. This can be done by layering the cell suspension over a separation medium and centrifuging at room temperature as per the instructions provided. Live cells will form a layer at the interface, while the dead cells pellet. The interface can then be collected and washed in Cytotoxicity Medium before being resuspended in the appropriate medium for further processing. Alternatively, the cells can be washed and resuspended in the appropriate medium for further processing immediately after Step #6, provided that the dead cells will not interfere with subsequent assays.

FUNCTIONAL TESTING: Method:
Cells were treated as described in "Cytotoxicity Depletion Assay" above. Treated cells and controls were tested for:
a) the ability to generate plaque-forming cells (PFC) using a modified Jerne haemolytic plaque assay.
b) the ability to generate cytotoxic T effector cells using a cytotoxic lymphocyte reaction (CTL) assay. Cells were treated both before and after sensitization in the CTL assay.
Results:
Cell Source: Splenocytes
Donors: AKR/J and BALB/c
Cell Concentration: 1x107 cells/ml
Antibody Concentration Used: 1:5
Complement: Low-Tox®-M Rabbit Complement
Complement Concentration Used: 1:10
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 Testing Data of anti-CD90 (Thy 1.1) antibody    anti-CD90 (Thy 1.1) antibody Testing Data image
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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