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anti-CDC2/CDK1 antibody :: anti-Human CDC2/CDK1 Monoclonal Antibody

Scan QR to view Datasheet Catalog #    MBS475068
Unit / Price
0.1 mL  /  $335 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

CDC2/CDK1, Monoclonal Antibody

 Also Known As   

Anti-CDC2/CDK1 Mouse mAb

 Product Synonym Names    Cdc 2; Cdc2; CDC28A; CDK 1; CDK1; CDK1_HUMAN; CDKN1; CELL CYCLE CONTROLLER CDC2; Cell division control protein 2; Cell division control protein 2 homolog; Cell division cycle 2 G1 to S and G2 to M; Cell division protein kinase 1; Cell Divsion Cycle 2 Protein; Cyclin Dependent Kinase 1; Cyclin-dependent kinase 1; DKFZp686L20222; MGC111195; p34 Cdk1; p34 protein kinase; P34CDC2
 Product Gene Name   

anti-CDC2/CDK1 antibody

[Similar Products]
 Product Synonym Gene Name    cdk1; CDC2; CDC28A; P34CDC2 [Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 OMIM    X05360 mRNA
 3D Structure    ModBase 3D Structure for P06493
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 Clonality    Monoclonal
 Isotype    IgG2b
 Clone Number    3B9-F8-B12
 Host    Mouse
Source: Human
 Species Reactivity    Human
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 Purity/Purification    Affinity Purified
 Form/Format    Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.2% sodium azide, 50%, glycerol
 Concentration    1mg/ml (lot specific)
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 Immunogen    Purified recombinant human CDC2/CDK1 protein fragments expressed in E Coli.
 Sensitivity    This antibody detects endogenous levels of CDC2/CDK1 and does not cross-react with related proteins.
 Sequence Similarities    Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily. Contains 1 protein kinase domain.
 Subunit Structure    Forms a stable but non-covalent complex with a regulatory subunit and with a cyclin. Interacts with cyclins-B (CCNB1, CCNB2 and CCNB3) to form a serine/threonine kinase holoenzyme complex also known as maturation promoting factor (MPF). The cyclin subunit imparts substrate specificity to the complex. Can also form CDK1-cylin-D and CDK1-cyclin-E complexes that phosphorylate RB1 in vitro. Binds to RB1 and other transcription factors such as FOXO1 and RUNX2. Promotes G2-M transition when in complex with a cyclin-B. Interacts with DLGAP5. Binds to the CDK inhibitors CDKN1A/p21 and CDKN1B/p27. Isoform 2 is unable to complex with cyclin-B1 and also fails to bind to CDKN1A/p21. Interacts with catalytically active CCNB1 and RALBP1 during mitosis to form an endocytotic complex during interphase. Associates with cyclins-A and B1 during S-phase in regenerating hepatocytes. Interacts with FANCC.
 Subcellular Location    Nucleus. Cytoplasm
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 Other Notes    Small volumes of anti-CDC2/CDK1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for anti-CDC2/CDK1 antibody

   Entrez Summary: The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. Mitotic cyclins stably associate with this protein and function as regulatory subunits. The kinase activity of this protein is controlled by cyclin accumulation and destruction through the cell cycle. The phosphorylation and dephosphorylation of this protein also play important regulatory roles in cell cycle control. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
 Product Categories/Family for anti-CDC2/CDK1 antibody    Epigenetics and Nuclear Signaling; Cell cycle; Kinases/Phosphatases; Cdks; Neuroscience; Neurology process; Neurodegenerative disease; Other
 Applications Tested/Suitable for anti-CDC2/CDK1 antibody   

Western Blot (WB), Immunocytochemistry (ICC)

 Application Notes for anti-CDC2/CDK1 antibody    WB: 1:100
ICC: 1:50
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 Western Blot (WB) of anti-CDC2/CDK1 antibody    Western blot detection of CDC2/CDK1 in K562, A549, Jurkat and Hela cell lysates using CDC2/CDK1 mouse mAb (1:100 diluted).Predicted band size: 34KDa.Observed band size: 34KDa.
anti-CDC2/CDK1 antibody Western Blot (WB) (WB) image
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 Immunocytochemistry (ICC) of anti-CDC2/CDK1 antibody    Immunocytochemistry of HeLa cells using anti-CDC2/CDK1 mouse mAb diluted 1:50.
anti-CDC2/CDK1 antibody Immunocytochemistry (ICC) (ICC) image
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NCBI/Uniprot data below describe general gene information for CDC2/CDK1. It may not necessarily be applicable to this product.
 NCBI GI #    1004170672
 NCBI GeneID    983
 NCBI Accession #    NP_001307847.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001130829.1 [Other Products]
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 UniProt Primary Accession #    P06493 [Other Products]
 UniProt Secondary Accession #    O60764; A8K7C4; C9J497 [Other Products]
 UniProt Related Accession #    P06493 [Other Products]
 Molecular Weight    34
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 NCBI Official Full Name    cyclin-dependent kinase 1 isoform 1
 NCBI Official Synonym Full Names    cyclin dependent kinase 1
 NCBI Official Symbol    CDK1 [Similar Products]
 NCBI Official Synonym Symbols   
CDC2; CDC28A; P34CDC2
[Similar Products]
 NCBI Protein Information    cyclin-dependent kinase 1
 UniProt Protein Name    Cyclin-dependent kinase 1
 UniProt Synonym Protein Names   
Cell division control protein 2 homolog; Cell division protein kinase 1; p34 protein kinase
 UniProt Gene Name    CDK1 [Similar Products]
 UniProt Synonym Gene Names    CDC2; CDC28A; CDKN1; P34CDC2; CDK1 [Similar Products]
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 NCBI Summary for CDC2/CDK1    The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. Mitotic cyclins stably associate with this protein and function as regulatory subunits. The kinase activity of this protein is controlled by cyclin accumulation and destruction through the cell cycle. The phosphorylation and dephosphorylation of this protein also play important regulatory roles in cell cycle control. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]
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 UniProt Comments for CDC2/CDK1    CDK1: a protein kinase of the CDK family. Catalytic subunit of the conserved protein complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions. Mitotic cyclins stably associate with this protein and function as regulatory subunits. Its activity is controlled by cyclin availability and phosphorylation through the cell cycle. Activated in many cancers including colon, liver and breast. The T isoform, which lacks a regulatory region, is expressed in breast cancer. Inhibition in cancer cells may drive cells into apoptosis. May also drive cell migration. Inhibitors: BMS-265246, BMS-265246-01. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: CDK family; CDK/CDK1 subfamily; CDK1 subfamily; CMGC group; Cell cycle regulation; EC 2.7.11.22; EC 2.7.11.23; Kinase, protein; Motility/polarity/chemotaxis; Protein kinase, CMGC; Protein kinase, Ser/Thr (non-receptor)

Chromosomal Location of Human Ortholog: 10q21.2

Cellular Component: centrosome; cytoplasm; cytosol; membrane; midbody; mitochondrion; nuclear chromosome, telomeric region; nucleoplasm; nucleus; spindle microtubule

Molecular Function: cyclin binding; cyclin-dependent protein kinase activity; histone kinase activity; protein binding; protein kinase activity; protein serine/threonine kinase activity; RNA polymerase subunit kinase activity

Biological Process: activation of MAPK activity; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; cell migration; centrosome cycle; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; DNA repair; DNA replication; epithelial cell differentiation; G1/S transition of mitotic cell cycle; G1/S-specific transcription in mitotic cell cycle; G2/M transition of mitotic cell cycle; microtubule cytoskeleton organization and biogenesis; mitotic nuclear envelope disassembly; negative regulation of apoptosis; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; peptidyl-serine phosphorylation; peptidyl-threonine phosphorylation; positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; pronuclear fusion; proteasomal ubiquitin-dependent protein catabolic process; protein ubiquitination during ubiquitin-dependent protein catabolic process; regulation of embryonic development; regulation of Schwann cell differentiation; regulation of ubiquitin-protein ligase activity during mitotic cell cycle
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 Research Articles on CDC2/CDK1    1. Ajuba is phosphorylated in vitro and in vivo by cyclin-dependent kinase 1 (CDK1) at Ser(119) and Ser(175) during the G2/M phase of the cell cycle
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-CDC2/CDK1 antibodyPathways associated with anti-CDC2/CDK1 antibody
 Reference Product  PubMed Publications
 CCNB1 antibody  >750 publications with CDC2/CDK1 and CCNB1
 CDC25C antibody  >209 publications with CDC2/CDK1 and CDC25C
 WEE1 antibody  >194 publications with CDC2/CDK1 and WEE1
 CDKN1A antibody  >191 publications with CDC2/CDK1 and CDKN1A
 CDC25B antibody  >87 publications with CDC2/CDK1 and CDC25B
 CCNA2 antibody  >77 publications with CDC2/CDK1 and CCNA2
 CDC25A antibody  >70 publications with CDC2/CDK1 and CDC25A
 CCNB2 antibody  >47 publications with CDC2/CDK1 and CCNB2
 CCNA1 antibody  >7 publications with CDC2/CDK1 and CCNA1
 RB1 antibody  >4 publications with CDC2/CDK1 and RB1
 Products by Pathway  Pathway Diagram
 APC/C-mediated Degradation Of Cell Cycle Proteins Pathway antibodies  APC/C-mediated Degradation Of Cell Cycle Proteins Pathway Diagram
 APC/C:Cdc20 Mediated Degradation Of Cyclin B Pathway antibodies  APC/C:Cdc20 Mediated Degradation Of Cyclin B Pathway Diagram
 APC/C:Cdc20 Mediated Degradation Of Mitotic Proteins Pathway antibodies  APC/C:Cdc20 Mediated Degradation Of Mitotic Proteins Pathway Diagram
 APC:Cdc20 Mediated Degradation Of Cell Cycle Proteins Prior To Satisfation Of The Cell Cycle Checkpoint Pathway antibodies  APC:Cdc20 Mediated Degradation Of Cell Cycle Proteins Prior To Satisfation Of The Cell Cycle Checkpoint Pathway Diagram
 AURKA Activation By TPX2 Pathway antibodies  AURKA Activation By TPX2 Pathway Diagram
 Activation Of APC/C And APC/C:Cdc20 Mediated Degradation Of Mitotic Proteins Pathway antibodies  Activation Of APC/C And APC/C:Cdc20 Mediated Degradation Of Mitotic Proteins Pathway Diagram
 Activation Of NIMA Kinases NEK9, NEK6, NEK7 Pathway antibodies  Activation Of NIMA Kinases NEK9, NEK6, NEK7 Pathway Diagram
 Anchoring Of The Basal Body To The Plasma Membrane Pathway antibodies  Anchoring Of The Basal Body To The Plasma Membrane Pathway Diagram
 Assembly Of The Primary Cilium Pathway antibodies  Assembly Of The Primary Cilium Pathway Diagram
 Cdc20:Phospho-APC/C Mediated Degradation Of Cyclin A Pathway antibodies  Cdc20:Phospho-APC/C Mediated Degradation Of Cyclin A Pathway Diagram
Diseases associated with anti-CDC2/CDK1 antibodyOrgans/Tissues associated with anti-CDC2/CDK1 antibody
 Disease Name  Pubmed Publications
 Adenocarcinoma Antibodies  >138 publications with CDC2/CDK1 and Adenocarcinoma
 Breast Neoplasms Antibodies  >116 publications with CDC2/CDK1 and Breast Neoplasms
 Lung Neoplasms Antibodies  >85 publications with CDC2/CDK1 and Lung Neoplasms
 Liver Diseases Antibodies  >83 publications with CDC2/CDK1 and Liver Diseases
 Carcinoma, Hepatocellular Antibodies  >82 publications with CDC2/CDK1 and Carcinoma, Hepatocellular
 Liver Neoplasms Antibodies  >76 publications with CDC2/CDK1 and Liver Neoplasms
 Prostatic Neoplasms Antibodies  >62 publications with CDC2/CDK1 and Prostatic Neoplasms
 Neoplasms, Experimental Antibodies  >59 publications with CDC2/CDK1 and Neoplasms, Experimental
 Cell Transformation, Neoplastic Antibodies  >40 publications with CDC2/CDK1 and Cell Transformation, Neoplastic
 Colonic Neoplasms Antibodies  >37 publications with CDC2/CDK1 and Colonic Neoplasms
 Organ/Tissue Name  Pubmed Publications
 Lung Antibodies  >144 publications with CDC2/CDK1 and Lung
 Liver Antibodies  >141 publications with CDC2/CDK1 and Liver
 Blood Antibodies  >106 publications with CDC2/CDK1 and Blood
 Brain Antibodies  >98 publications with CDC2/CDK1 and Brain
 Prostate Antibodies  >96 publications with CDC2/CDK1 and Prostate
 Bone Antibodies  >66 publications with CDC2/CDK1 and Bone
 Vascular Antibodies  >50 publications with CDC2/CDK1 and Vascular
 Muscle Antibodies  >48 publications with CDC2/CDK1 and Muscle
 Kidney Antibodies  >46 publications with CDC2/CDK1 and Kidney
 Skin Antibodies  >38 publications with CDC2/CDK1 and Skin
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