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anti-COBRA1 antibody :: Mouse COBRA1 (NELF-B) Monoclonal Antibody

Scan QR to view Datasheet Catalog #    MBS500037
Testing Data
Unit / Price
0.1 mL  /  $345 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

COBRA1 (NELF-B), Monoclonal Antibody

 Also Known As   

Anti-COBRA 1

 Product Gene Name   

anti-COBRA1 antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Chromosome Location    Chromosome: 9; NC_000009.12 (137255307..137273548). Location: 9q34
 OMIM    611180
 3D Structure    ModBase 3D Structure for Q8WX92
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 Clonality    Monoclonal
 Isotype    IgG1
 Clone Number    F7E4
 Host    Mouse
 Species Reactivity    Human, mouse, rat
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 Specificity    Specific for the ~62k COBRA1 protein in Western blots of neonatal rat brain lysate.
 Purity/Purification    Protein G purified (Protein G purified culture supernatant)
 Form/Format    100 ul in PBS + 0.05 % NaN3. Adequate amount of material to conduct 10-mini Western Blots.
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 Antigen    Recombinant full length human COBRA1 purified from E. coli
 Immunogen Information    Recombinant full length human COBRA1 purified from E. coli
 Immunogen Species    Human
 Species Reactivity Note    The antibody has been directly tested for reactivity in Western blots with human, mouse and rat tissue.
 Biological Significance    COBRA1 (cofactor of BRCA1) also known as NELF-B, is an essential component of the negative elongation factor (NELF) complex. COBRA1 has been shown to directly bind estrogen receptor alpha (ERalpha) and repress ERalpha-mediated transcription (Aiyar SE et al., 2004). COBRA1 has also been demonstrated to interact with c-Jun and c-Fos suggesting a role in modulation of the AP-1 pathway and therefore may be important in cell proliferation, differentiation, apoptosis and oncogeneis (Zhong H et al., 2004)
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 Preparation and Storage    Store at -20 degree C; stable for at least one year
 Other Notes    Small volumes of anti-COBRA1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Product Description specifically for anti-COBRA1 antibody

   Purified mouse monoclonal antibody
 Applications Tested/Suitable for anti-COBRA1 antibody   

Western Blot (WB)

 Application Notes for anti-COBRA1 antibody    Quality Control: Western blots performed on each lot.
WB: 1:1000
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 Testing Data of anti-COBRA1 antibody    anti-COBRA1 antibody Testing Data image
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NCBI/Uniprot data below describe general gene information for COBRA1. It may not necessarily be applicable to this product.
 NCBI GI #    20070260
 NCBI GeneID    25920
 NCBI Accession #    NP_056271.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_015456.3 [Other Products]
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 UniProt Primary Accession #    Q8WX92 [Other Products]
 UniProt Secondary Accession #    Q96EW5; Q9H9R4; Q9ULN8; Q9Y3W0; A2BFA3 [Other Products]
 UniProt Related Accession #    Q8WX92 [Other Products]
 Molecular Weight    62
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 NCBI Official Full Name    negative elongation factor B
 NCBI Official Synonym Full Names    negative elongation factor complex member B
 NCBI Official Symbol    NELFB [Similar Products]
 NCBI Official Synonym Symbols   
COBRA1; NELF-B; RP13-122B23.3
[Similar Products]
 NCBI Protein Information    negative elongation factor B; cofactor of BRCA1; negative elongation factor protein B
 UniProt Protein Name    Negative elongation factor B
 UniProt Synonym Protein Names   
Cofactor of BRCA1
 UniProt Gene Name    NELFB [Similar Products]
 UniProt Synonym Gene Names    COBRA1; KIAA1182; NELF-B [Similar Products]
 UniProt Entry Name    NELFB_HUMAN
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 NCBI Summary for COBRA1    NELFB is a subunit of negative elongation factor (NELF), which also includes NELFA (WHSC2; MIM 606026), either NELFC or NELFD (TH1L; MIM 605297), and NELFE (RDBP; MIM 154040). NELF acts with DRB sensitivity-inducing factor (DSIF), a heterodimer of SPT4 (SUPT4H1; MIM 603555) and SPT5 (SUPT5H; MIM 602102), to cause transcriptional pausing of RNA polymerase II (see MIM 180660) (Narita et al., 2003 [PubMed 12612062]).[supplied by OMIM, Mar 2008]
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 UniProt Comments for COBRA1    Function: Essential component of the NELF complex, a complex that negatively regulates the elongation of transcription by RNA polymerase II. The NELF complex, which acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex. May be able to induce chromatin unfolding. Ref.7 Ref.8

Subunit structure: The NELF complex is composed of NELFA, NELFB, NELFCD (isoform NELF-Cor isoform NELF-D)and NELFE. Interacts with the first BRCT repeat of BRCA1. Interacts with KIAA1191. Ref.1 Ref.7 Ref.15

Subcellular location: Nucleus Ref.1.

Tissue specificity: Widely expressed. Expressed in heart, brain, lung, placenta, liver, skeletal muscle, kidney and pancreas. Ref.7

Sequence similarities: Belongs to the NELF-B family.

Sequence caution: The sequence AAH11892.1 differs from that shown. Reason: Erroneous initiation. The sequence BAB14157.1 differs from that shown. Reason: Erroneous initiation. The sequence CAB43381.3 differs from that shown. Reason: Erroneous initiation.
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Product References and Citations for anti-COBRA1 antibody

   • Aiyar, SE, Sun J, Blair AL, Moskaluk CA, Lu Y, Ye Q, Yamaguchi Y, Mukherjee A, Ren D, Handa H, Li R (2004). Attenuation of estrogen receptor alpha-mediated transcription through estrogen-stimulated recruitment of a negative elongation factor. Genes Dev. 18 (17): 2134- 46.
• Zhong, H, Zhu J, Zhang H, Ding L, Sun Y, Huang C, Ye Qi (2004). COBRA1 inhibits AP-1 transcriptional activity in transfected cells. Biochem. Biophys. Res. Commun.325 (2): 568- 73.
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 Research Articles on COBRA1    1. These studies allow us to position the actions of two new modulators of GR-regulated transactivation, NELF-A and NELF-B, relative to other factors in the overall gene induction sequence.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with anti-COBRA1 antibody
 Products by Pathway  Pathway Diagram
 Abortive Elongation Of HIV-1 Transcript In The Absence Of Tat Pathway antibodies  Abortive Elongation Of HIV-1 Transcript In The Absence Of Tat Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 Formation Of HIV Elongation Complex In The Absence Of HIV Tat Pathway antibodies  Formation Of HIV Elongation Complex In The Absence Of HIV Tat Pathway Diagram
 Formation Of HIV-1 Elongation Complex Containing HIV-1 Tat Pathway antibodies  Formation Of HIV-1 Elongation Complex Containing HIV-1 Tat Pathway Diagram
 Formation Of RNA Pol II Elongation Complex Pathway antibodies  Formation Of RNA Pol II Elongation Complex Pathway Diagram
 Formation Of The Early Elongation Complex Pathway antibodies  Formation Of The Early Elongation Complex Pathway Diagram
 Formation Of The HIV-1 Early Elongation Complex Pathway antibodies  Formation Of The HIV-1 Early Elongation Complex Pathway Diagram
 Gene Expression Pathway antibodies  Gene Expression Pathway Diagram
 HIV Infection Pathway antibodies  HIV Infection Pathway Diagram
 HIV Life Cycle Pathway antibodies  HIV Life Cycle Pathway Diagram
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