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anti-CRB3 antibody :: Rat CRB3 Monoclonal Antibody

Scan QR to view Datasheet Catalog #    MBS588104
Unit / Price
0.1 mg  /  $555 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

CRB3, Monoclonal Antibody

 Also Known As   

Rat anti-CRB3 (14F9)

 Product Gene Name   

anti-CRB3 antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 OMIM    609737
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 Clonality    Monoclonal
 Isotype    IgG2c
 Clone Number    14F9
 Host    Rat
 Species Reactivity    The antibody recognizes the CRB3 protein.
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 Specificity    Specific for: human and mouse; other species not tested.
 Form/Format    100 ug of lyophilized, purified antibody. Reconstitute in 100 ul of distilled H2O for a 1 ug/ul solution. It contains no additives.
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 Limitations    This product is to be used for research purposes only.
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 Preparation and Storage    Lyophilized antibody can be kept at 4 degree C for up to 3 months and should be kept at -20 degree C for long-term storage. To avoid freeze-thaw cycles, reconstituted antibody should be aliquoted before freezing for short-term storage (-20 degree C) or for long-term storage (-80 degree C). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
Stability: Minimum 1 year from reception date.
 Other Notes    Small volumes of anti-CRB3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Product Description specifically for anti-CRB3 antibody

   CRB3 (Crumbs homologue 3) is an apical transmembrane protein member of the CRB protein family reported to be associated with tight junctions at the apical surface of epithelial cells and to play a role in apical membrane morphogenesis and tight junction regulation. The formation and maintenance of tight junctions is essential for the development of epithelial cell polarity. Two CRB3 isoforms have been described and CRB3 is homologous to the Crumb protein found in Drosophila. CRB3 contains a putative signal peptide in its first 20 amino acids, a putative extracellular site, and a transmembrane domain. The intracellular domain contains a PDZ-binding motif which is involved in the interactions of CRB3 with PARD6A and MPP5. Loss of CRB3 expression in immortal baby mouse kidney (iBMK) cells was reported to render these cells tumorigenic, suggesting an important role for CRB3 function in maintaining epithelial junction formation and apicobasal polarity required for contact-inhibited growth, and to suppress invasion and metastasis.
 Applications Tested/Suitable for anti-CRB3 antibody   

ELISA (E), Western blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

 Application Notes for anti-CRB3 antibody    If reconstituted in 100 ul: WB 1:1000, IHC 1:100, IF 1:100. Optimal dilution has to be determined by the user.
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 Western blot of anti-CRB3 antibody    Western blot analysis of full length CRB3 (GST-CRB3) but not the extracellular domain of CRB3 (GST-CRB3extra) (anti-CRB3 (14F9) was used at 1:1000).
anti-CRB3 antibody Western blot image
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 Western blot of anti-CRB3 antibody    Western blot analysis of overexpressed (HEK293T cells) and endogenous CRB3 (mouse tissues).
anti-CRB3 antibody Western blot image
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 Immunofluorescence of anti-CRB3 antibody    Immunofluorescence analysis of overexpressed human CRB3 in lysates of HeLa cells.
anti-CRB3 antibody Immunofluorescence image
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 Immunofluorescence of anti-CRB3 antibody    Immunofluorescence analysis of endogenous human CRB3 expression in the mouse intestine. DAPI was used to stain the nuclei in HeLa cells, whereas E-cadherin staining was used to highlight the lateral membrane in mouse intestine.
anti-CRB3 antibody Immunofluorescence image
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NCBI/Uniprot data below describe general gene information for CRB3. It may not necessarily be applicable to this product.
 NCBI GI #    37182490
 NCBI GeneID    92359
 NCBI Accession #    AAQ89047.1 [Other Products]
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 UniProt Secondary Accession #    Q8N0V8; Q8WVA0; A8KA91; D6W643 [Other Products]
 UniProt Related Accession #    Q9BUF7 [Other Products]
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 NCBI Official Full Name    CRB3
 NCBI Official Synonym Full Names    crumbs family member 3
 NCBI Official Symbol    CRB3 [Similar Products]
 NCBI Protein Information    protein crumbs homolog 3; crumbs protein homolog 3
 UniProt Protein Name    Protein crumbs homolog 3
 Protein Family    Protein crumbs
 UniProt Gene Name    CRB3 [Similar Products]
 UniProt Entry Name    CRUM3_HUMAN
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 NCBI Summary for CRB3    This gene encodes a member of the Crumbs family of proteins. This protein may play a role in epithelial cell polarity and is associated with tight junctions at the apical surface of epithelial cells. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]
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 UniProt Comments for CRB3    CRB3: Involved in the establishment of cell polarity in mammalian epithelial cells. Regulates the morphogenesis of tight junctions. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell adhesion; Membrane protein, integral; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: tight junction; protein complex; apical plasma membrane; plasma membrane; integral to membrane; cell junction

Molecular Function: protein domain specific binding; SH3 domain binding

Biological Process: intercellular junction assembly and maintenance
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 Research Articles on CRB3    1. Morg1 facilitates Par6-aPKC binding to Crb3 for definition of apical identity of epithelial cells.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-CRB3 antibodyPathways associated with anti-CRB3 antibody
 Reference Product  PubMed Publications
 CRB1 antibody  >14 publications with CRB3 and CRB1
 INADL antibody  >10 publications with CRB3 and INADL
 MPP5 antibody  >9 publications with CRB3 and MPP5
 CRB2 antibody  >8 publications with CRB3 and CRB2
 FOS antibody  >5 publications with CRB3 and FOS
 EPB41L5 antibody  >1 publications with CRB3 and EPB41L5
 PARD6A antibody  >1 publications with CRB3 and PARD6A
 Products by Pathway  Pathway Diagram
 Cell Junction Organization Pathway antibodies  Cell Junction Organization Pathway Diagram
 Cell-Cell Communication Pathway antibodies  Cell-Cell Communication Pathway Diagram
 Cell-cell Junction Organization Pathway antibodies  Cell-cell Junction Organization Pathway Diagram
 Tight Junction Pathway antibodies  Tight Junction Pathway Diagram
 Tight Junction Pathway antibodies  Tight Junction Pathway Diagram
 Tight Junction Interactions Pathway antibodies  Tight Junction Interactions Pathway Diagram
Diseases associated with anti-CRB3 antibodyOrgans/Tissues associated with anti-CRB3 antibody
 Disease Name  Pubmed Publications
 Liver Diseases Antibodies  >133 publications with CRB3 and Liver Diseases
 Inflammation Antibodies  >35 publications with CRB3 and Inflammation
 Carcinoma, Hepatocellular Antibodies  >28 publications with CRB3 and Carcinoma, Hepatocellular
 Liver Neoplasms Antibodies  >18 publications with CRB3 and Liver Neoplasms
 Male Urogenital Diseases Antibodies  >17 publications with CRB3 and Male Urogenital Diseases
 Necrosis Antibodies  >17 publications with CRB3 and Necrosis
 Fatty Liver Antibodies  >10 publications with CRB3 and Fatty Liver
 Eye Diseases Antibodies  >6 publications with CRB3 and Eye Diseases
 Lung Diseases Antibodies  >6 publications with CRB3 and Lung Diseases
 Hyperplasia Antibodies  >4 publications with CRB3 and Hyperplasia
 Organ/Tissue Name  Pubmed Publications
 Kidney Antibodies  >33 publications with CRB3 and Kidney
 Intestine Antibodies  >19 publications with CRB3 and Intestine
 Lung Antibodies  >17 publications with CRB3 and Lung
 Eye Antibodies  >16 publications with CRB3 and Eye
 Pancreas Antibodies  >9 publications with CRB3 and Pancreas
 Embryonic Tissue Antibodies  >7 publications with CRB3 and Embryonic Tissue
 Stomach Antibodies  >4 publications with CRB3 and Stomach
 Ovary Antibodies  >3 publications with CRB3 and Ovary
 Uterus Antibodies  >2 publications with CRB3 and Uterus
 Connective Tissue Antibodies  >2 publications with CRB3 and Connective Tissue
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