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anti-CACNA1H antibody :: Mouse Cav3.2 Monoclonal Antibody

Scan QR to view Datasheet Catalog #    MBS803808
Unit / Price
0.1 mg  /  $425 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Cav3.2 (CACNA1H), Monoclonal Antibody

★Popular Item★
 Also Known As   

CaV3.2 Antibody: HRP

 Product Synonym Names    Cav3.2; CACNA1H; CACNA1HB; calcium channel; voltage-dependent; T type; alpha 1H subunit; calcium channel; voltage-dependent; T type; alpha 1Hb subunit; ECA6; EIG6; FLJ90484; Low-voltage-activated calcium channel alpha1 3.2 subunit
 Product Gene Name   

anti-CACNA1H antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 OMIM    gene 611942
 3D Structure    ModBase 3D Structure for O95180
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 Clonality    Monoclonal
 Isotype    IgG1
 Clone Number    S55-10
 Host    Mouse
 Species Reactivity    Human, Mouse, Rat.
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 Specificity    Detects ~260kDa. No cross-reactivity against Cav1.3
 Form/Format    Protein G Purified
 Concentration    1mg/mL (lot specific)
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 Conjugate    HRP
 Immmunogen    Fusion protein amino acids 1019-1293 (II-III loop} of human Cav3.2
 Storage Buffer    PBS pH7.4, 50% glycerol, 0.09% sodium azide
 Certificate of Analysis    1 ug/ml of SMC-303 was sufficient for detection of Cav3.2 in 10ug of HEK cell lysate expressing Cav3.2 by colorimetric immunoblot analysis using Goat anti-mouse IgG: HRP as the secondary antibody.
 Research Area(s)    Neuroscience
Ion Channels
Calcium Channels
Voltage-Gated Calcium Channels
Cancer
Cell Signaling
 Cellular Localization    Membrane
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 Preparation and Storage    -20 degree C; 1 year +
Avoid freeze/thaw cycles.
 Other Notes    Small volumes of anti-CACNA1H antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for anti-CACNA1H antibody

   Background Info: Detects ~260kDa. No cross-reactivity against Cav1.3 or Cav3.1

Scientific Background: Ion channels are integral membrane proteins that help establish and control the small voltage gradient across the plasma membrane of living cells by allowing the flow of ions down their electrochemical gradient (1). They are present in the membranes that surround all biological cells because their main function is to regulate the flow of ions across this membrane. Whereas some ion channels permit the passage of ions based on charge, others conduct based on a ionic species, such as sodium or potassium. Furthermore, in some ion channels, the passage is governed by a gate which is controlled by chemical or electrical signals, temperature, or mechanical forces. There are a few main classifications of gated ion channels. There are voltage- gated ion channels, ligand-gated, other gating systems and finally those that are classified differently, having more exotic characteristics. The first are voltage- gated ion channels which open and close in response to membrane potential. These are then separated into sodium, calcium, potassium, proton, transient receptor, and cyclic nucleotide-gated channels; each of which is responsible for a unique role. Ligand-gated ion channels are also known as ionotropic receptors, and they open in response to specific ligand molecules binding to the extracellular domain of the receptor protein. The other gated classifications include activation and inactivation by second messengers, inward-rectifier potassium channels, calcium-activated potassium channels, two-pore-domain potassium channels, light-gated channels, mechano-sensitive ion channels and cyclic nucleotide-gated channels. Finally, the other classifications are based on less normal characteristics such as two-pore channels, and transient receptor potential channels (2). Specifically, CaV3.2 is a protein which in humans is encoded by the CACNA1H gene. Studies suggest certain mutations in this gene lead to childhood absence epilepsy (3, 4). Studies also suggest that the up-regulations of CaV3.2 may participate in the progression of prostate cancer toward an androgen-independent stage (5).
 Product Categories/Family for anti-CACNA1H antibody    Ion Channels, Neuroscience
 Applications Tested/Suitable for anti-CACNA1H antibody   

Western Blot (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunofluorescence (IF)

 Application Notes for anti-CACNA1H antibody    WB (1:1000), IHC (1:1000), ICC/IF (1:100); Optimal dilutions for assays should be determined by the user.
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 Immunohistochemistry (IHC) of anti-CACNA1H antibody    Immunohistochemistry analysis using Mouse Anti-CaV3.2 Calcium Channel Monoclonal Antibody, Clone S55-10. Tissue: hippocampus. Species: Human. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-CaV3.2 Calcium Channel Monoclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT.
anti-CACNA1H antibody Immunohistochemistry (IHC) (IHC) image
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 Immunohistochemistry (IHC) of anti-CACNA1H antibody    Immunohistochemistry analysis using Mouse Anti-CaV3.2 Calcium channel Monoclonal Antibody, Clone S55-10. Tissue: frozen brain section. Species: human. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-CaV3.2 Calcium channel Monoclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 ul for 5 minutes at RT.
anti-CACNA1H antibody Immunohistochemistry (IHC) (IHC) image
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NCBI/Uniprot data below describe general gene information for CACNA1H. It may not necessarily be applicable to this product.
 NCBI GI #    53832011
 NCBI GeneID    8912
 NCBI Accession #    NP_001005407.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001005407.1 [Other Products]
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 UniProt Primary Accession #    O95180 [Other Products]
 UniProt Secondary Accession #    O95802; Q8WWI6; Q96QI6; Q96RZ9; Q9NYY4; Q9NYY5; B5ME00; F8WFD1 [Other Products]
 UniProt Related Accession #    O95180 [Other Products]
 Molecular Weight    258,545 Da [Similar Products]
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 NCBI Official Full Name    voltage-dependent T-type calcium channel subunit alpha-1H isoform b
 NCBI Official Synonym Full Names    calcium channel, voltage-dependent, T type, alpha 1H subunit
 NCBI Official Symbol    CACNA1H [Similar Products]
 NCBI Official Synonym Symbols   
ECA6; EIG6; Cav3.2; CACNA1HB
[Similar Products]
 NCBI Protein Information    voltage-dependent T-type calcium channel subunit alpha-1H; voltage-gated calcium channel alpha subunit Cav3.2; voltage-gated calcium channel alpha subunit CavT.2; voltage-gated calcium channel subunit alpha Cav3.2; low-voltage-activated calcium channel alpha13.2 subunit; low-voltage-activated calcium channel alpha1 3.2 subunit; voltage dependent t-type calcium channel alpha-1H subunit; calcium channel, voltage-dependent, T type, alpha 1Hb subunit
 UniProt Protein Name    Voltage-dependent T-type calcium channel subunit alpha-1H
 UniProt Synonym Protein Names   
Low-voltage-activated calcium channel alpha1 3.2 subunit; Voltage-gated calcium channel subunit alpha Cav3.2
 Protein Family    Voltage-dependent T-type calcium channel
 UniProt Gene Name    CACNA1H [Similar Products]
 UniProt Entry Name    CAC1H_HUMAN
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 NCBI Summary for CACNA1H    This gene encodes a T-type member of the alpha-1 subunit family, a protein in the voltage-dependent calcium channel complex. Calcium channels mediate the influx of calcium ions into the cell upon membrane polarization and consist of a complex of alpha-1, alpha-2/delta, beta, and gamma subunits in a 1:1:1:1 ratio. The alpha-1 subunit has 24 transmembrane segments and forms the pore through which ions pass into the cell. There are multiple isoforms of each of the proteins in the complex, either encoded by different genes or the result of alternative splicing of transcripts. Alternate transcriptional splice variants, encoding different isoforms, have been characterized for the gene described here. Studies suggest certain mutations in this gene lead to childhood absence epilepsy (CAE). [provided by RefSeq, Jul 2008]
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 UniProt Comments for CACNA1H    CACNA1H: Voltage-sensitive calcium channels (VSCC) mediate the entry of calcium ions into excitable cells and are also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, gene expression, cell motility, cell division and cell death. The isoform alpha-1H gives rise to T-type calcium currents. T-type calcium channels belong to the low-voltage activated (LVA) group and are strongly blocked by nickel and mibefradil. A particularity of this type of channels is an opening at quite negative potentials, and a voltage-dependent inactivation. T-type channels serve pacemaking functions in both central neurons and cardiac nodal cells and support calcium signaling in secretory cells and vascular smooth muscle. They may also be involved in the modulation of firing patterns of neurons which is important for information processing as well as in cell growth processes. Defects in CACNA1H are a cause of susceptibility to epilepsy, idiopathic generalized type 6 (EIG6). A disorder characterized by recurring generalized seizures in the absence of detectable brain lesions and/or metabolic abnormalities. Generalized seizures arise diffusely and simultaneously from both hemispheres of the brain. EIG6 is a polygenic and multifactorial disease. Defects in CACNA1H are a cause of susceptibility to epilepsy, childhood absence type 6 (ECA6). A subtype of idiopathic generalized epilepsy characterized by an onset at age 6-7 years, frequent absence seizures (several per day) and bilateral, synchronous, symmetric 3-Hz spike waves on EEG. Tonic- clonic seizures often develop in adolescence. Absence seizures may either remit or persist into adulthood. Belongs to the calcium channel alpha-1 subunit (TC 1.A.1.11) family. CACNA1H subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, multi-pass; Channel, calcium; Cell development/differentiation; Membrane protein, integral

Chromosomal Location of Human Ortholog: 16p13.3

Cellular Component: plasma membrane; integral to membrane; voltage-gated calcium channel complex; caveola; sarcolemma

Molecular Function: low voltage-gated calcium channel activity; metal ion binding

Biological Process: axon guidance; regulation of membrane potential; muscle development; muscle contraction; transport; aldosterone biosynthetic process; myoblast fusion; regulation of heart contraction; cellular response to hormone stimulus

Disease: Epilepsy, Childhood Absence, Susceptibility To, 6
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Product References and Citations for anti-CACNA1H antibody

   1. Hille B. (2001) Ion Channels of Excitable Membranes, 3rd Ed., Sinauer Associated Inc.:Sunderland, MA USA. 2. www.iochannels.org 3. Chen Y., et al. (2003) Ann. Neurol. 54(2): 239-43. 4. Khosravani H., et al. (2004) J Biol Chem. 279(11): 9681- 9684. 5. Gackiere F., et al. (2008) J Biol Chem. 283(28): 19872.
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 Research Articles on CACNA1H    1. N-linked glycosylation of Cav3.2 not only controls surface expression.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-CACNA1H antibodyPathways associated with anti-CACNA1H antibody
 Reference Product  PubMed Publications
 CACNA1G antibody  >34 publications with CACNA1H and CACNA1G
 CACNA1I antibody  >19 publications with CACNA1H and CACNA1I
 CACNA1C antibody  >3 publications with CACNA1H and CACNA1C
 CACNA1F antibody  >1 publications with CACNA1H and CACNA1F
 CACNB1 antibody  >1 publications with CACNA1H and CACNB1
 Products by Pathway  Pathway Diagram
 Axon Guidance Pathway antibodies  Axon Guidance Pathway Diagram
 Calcium Signaling Pathway antibodies  Calcium Signaling Pathway Diagram
 Calcium Signaling Pathway antibodies  Calcium Signaling Pathway Diagram
 Circadian Entrainment Pathway antibodies  Circadian Entrainment Pathway Diagram
 Circadian Entrainment Pathway antibodies  Circadian Entrainment Pathway Diagram
 Corticotropin-releasing Hormone Pathway antibodies  Corticotropin-releasing Hormone Pathway Diagram
 Developmental Biology Pathway antibodies  Developmental Biology Pathway Diagram
 MAPK Signaling Pathway antibodies  MAPK Signaling Pathway Diagram
 MAPK Signaling Pathway antibodies  MAPK Signaling Pathway Diagram
 NCAM Signaling For Neurite Out-growth Pathway antibodies  NCAM Signaling For Neurite Out-growth Pathway Diagram
Diseases associated with anti-CACNA1H antibodyOrgans/Tissues associated with anti-CACNA1H antibody
 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >36 publications with CACNA1H and Nervous System Diseases
 Brain Diseases Antibodies  >29 publications with CACNA1H and Brain Diseases
 Epilepsy, Absence Antibodies  >23 publications with CACNA1H and Epilepsy, Absence
 Seizures Antibodies  >12 publications with CACNA1H and Seizures
 Pain Antibodies  >12 publications with CACNA1H and Pain
 Autistic Disorder Antibodies  >5 publications with CACNA1H and Autistic Disorder
 Heart Diseases Antibodies  >4 publications with CACNA1H and Heart Diseases
 Hyperalgesia Antibodies  >4 publications with CACNA1H and Hyperalgesia
 Inflammation Antibodies  >3 publications with CACNA1H and Inflammation
 Breast Neoplasms Antibodies  >2 publications with CACNA1H and Breast Neoplasms
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >25 publications with CACNA1H and Brain
 Kidney Antibodies  >12 publications with CACNA1H and Kidney
 Embryonic Tissue Antibodies  >3 publications with CACNA1H and Embryonic Tissue
 Intestine Antibodies  >3 publications with CACNA1H and Intestine
 Ovary Antibodies  >2 publications with CACNA1H and Ovary
 Prostate Antibodies  >2 publications with CACNA1H and Prostate
 Uterus Antibodies  >2 publications with CACNA1H and Uterus
 Lung Antibodies  >1 publications with CACNA1H and Lung
 Testis Antibodies  >1 publications with CACNA1H and Testis
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