Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
1.855.MyBioSource
Fax 1.858.633.0166    Email sales@mybiosource.com 
tel/fax
menu1 Home Products Ordering Company Help menu7
bottom
   MyBioSource rightarrow Antibody rightarrow COL4a5 rightarrow Monoclonal COL4a5  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Menu separator
separator
ELISA Kit ELISA Kit
CLIA Kit CLIA Kit
PCR Kit PCR Kit
Monoclonal Antibody Monoclonal Antibody
Polyclonal Antibody Polyclonal Antibody
Secondary Antibody Secondary Antibody
Antigen Antigen
Biochemical Biochemical
cDNA Clone cDNA Clone
siRNA siRNA
Peptide Peptide
Recombinant/Purified Protein Rec./Purified Protein

Custom ELISA Kit Custom ELISA Kit
Custom Protein Custom Protein
Custom Antibody Custom Antibody
Antibody Matched Pairs Antibody Matched Pairs
Antibody & Corresponding Blocking Peptide Pairs Antibody Peptide Pairs
Phospho-Specific Antibodies Phospho Antibodies
Products by Disease Products by Disease
Products by Pathway Products by Pathway
Products by Tissue Products by Tissue

arrow Advanced Search
arrow Submit Technical Q&A
arrow International Distributors
arrow Contact Us
separator
Our Best Sellers moreseparator
separator
 • HP Antibody
 • ApoC3 ELISA Kit
 • Myosin Heavy Chain (MHC) ELISA Kit
 • MARVEL domain containing 2 (MARVELD2) Antibody
 • Serpin B3 (SERPINB3) Antibody
 • Protein ROS1 (ROS1), partial Recombinant Protein
 • Retinoic Acid ELISA Kit
 • Cytochrome c oxidase subunit 5B (COX5B) Antibody
 • NDUFS7 Antibody
 • HDAC8 Antibody
 • C Reactive Protein (CRP) >95% Pure Native Protein
 • SNAI 1 Antibody
 • Cerebellin-4 (CBLN4) ELISA Kit
 • Fizzy-related (FZR1) Antibody
 downarrow more ...
separator
separator
DatasheetFull DatasheetPrinter Friendly DatasheetPrint This DatasheetAdd to Compare ListHave Questions? Ask UsRequest for a Quotation today

anti-COL4a5 antibody :: Mouse Collagen Type IV Alpha 5 Monoclonal Antibody

Scan QR to view Datasheet Catalog #    MBS2025713 (SPECIAL PROMOTION: Get FREE $5 Starbucks gift card (Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Western Blot (WB)
Unit / Price
0.01 mg  /  $105 +1 FREE 8GB USB
0.02 mg  /  $110 +1 FREE 8GB USB
0.05 mg  /  $145 +1 FREE 8GB USB
0.1 mg  /  $175 +1 FREE 8GB USB
0.2 mg  /  $230 +1 FREE 8GB USB
1 mg  /  $515 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Collagen Type IV Alpha 5 (COL4a5), Monoclonal Antibody

★Popular Item★
 Also Known As   

Monoclonal Antibody to Collagen Type IV Alpha 5 (COL4a5)

 Product Gene Name   

anti-COL4a5 antibody

[Similar Products]
 Matching Pairs    Antibody: Collagen Type IV Alpha 5 Clone #D2 (MBS2025713)
Antigen: Collagen Type IV Alpha 5 (COL4a5) (MBS2009288)
 Matching Pairs    Unconjugated Antibody: Collagen Type IV Alpha 5 Clone #D2 (MBS2025713)
APC-CY7 Conjugated Antibody: Collagen Type IV Alpha 5 (COL4a5) (MBS2063530)
 Matching Pairs    Unconjugated Antibody: Collagen Type IV Alpha 5 Clone #D2 (MBS2025713)
PE Conjugated Antibody: Collagen Type IV Alpha 5 (COL4a5) (MBS2063532)
 Matching Pairs    Unconjugated Antibody: Collagen Type IV Alpha 5 Clone #D2 (MBS2025713)
APC-CY7 Conjugated Antibody: Collagen Type IV Alpha 5 (COL4a5) (MBS2063534)
 Matching Pairs    Unconjugated Antibody: Collagen Type IV Alpha 5 Clone #D2 (MBS2025713)
Cy3 Conjugated Antibody: Collagen Type IV Alpha 5 (COL4a5) (MBS2063536)
 Matching Pairs    Unconjugated Antibody: Collagen Type IV Alpha 5 Clone #D2 (MBS2025713)
FITC Conjugated Antibody: Collagen Type IV Alpha 5 (COL4a5) (MBS2063539)
 Matching Pairs    Unconjugated Antibody: Collagen Type IV Alpha 5 Clone #D2 (MBS2025713)
HRP Conjugated Antibody: Collagen Type IV Alpha 5 (COL4a5) (MBS2063541)
 Matching Pairs    Unconjugated Antibody: Collagen Type IV Alpha 5 Clone #D2 (MBS2025713)
Biotin Conjugated Secondary Antibody: IgG (MBS2086030)
 Matching Pairs    Unconjugated Antibody: Collagen Type IV Alpha 5 Clone #D2 (MBS2025713)
FITC Conjugated Secondary Antibody: IgG (MBS2086031)
 Matching Pairs    Unconjugated Antibody: Collagen Type IV Alpha 5 Clone #D2 (MBS2025713)
Biotin Conjugated Secondary Antibody: IgG (MBS2086033)
 Matching Pairs    Unconjugated Antibody: Collagen Type IV Alpha 5 Clone #D2 (MBS2025713)
FITC Conjugated Secondary Antibody: IgG (MBS2086034)
 Matching Pairs    Unconjugated Antibody: Collagen Type IV Alpha 5 Clone #D2 (MBS2025713)
HRP Conjugated Secondary Antibody: IgG (MBS2086035)
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
Table BarTOPTable Bar
 MBS2025713 Technical Datasheet    Technical Datasheet PDF
 OMIM    M58526 mRNA
 3D Structure    ModBase 3D Structure for P29400
Section Bar
 Clonality    Monoclonal
 Isotype    IgG1 Kappa
 Clone Number    D2
 Host    Mouse
Section Bar
 Purity/Purification    Protein A + Protein G affinity chromatography
 Form/Format    Supplied as solution form in 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
 Concentration    1mg/mL (lot specific)
Section Bar
 Organism Species    Homo sapiens (Human)
 Source    Monoclonal antibody preparation
 Traits    Liquid
 Immunogen    Recombinant COL4a5 (Gly1461~Thr1685) expressed in E.coli
 Conjugate    No Conjugate
 Conjugated Antibody    The APC conjugated antibody version of this item is also available as catalog #MBS2063534
Section Bar
 Preparation and Storage    Storage: Avoid repeated freeze/thaw cycles.
Store at 4C for frequent use.
Aliquot and store at -20C for 12 months.

Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
 ISO Certification    Manufactured in an ISO 9001:2015 and ISO 13485:2016 Certified Laboratory.
 Supply Chain Verification    Manufactured in a lab with traceable raw materials manufactured on site. Coordinated product portfolio of antibodies, pairs, conjugates, recombinant proteins, and immunoassay materials available. Please inquire.
 Other Notes    Small volumes of anti-COL4a5 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Table BarTOPTable Bar
 Applications Tested/Suitable for anti-COL4a5 antibody   

WB; IHC; ICC; IP.

 Application Notes for anti-COL4a5 antibody    Western blotting: 0.5-2ug/mL;1:500-2000
Immunohistochemistry: 5-20ug/mL;1:50-200
Immunocytochemistry: 5-20ug/mL;1:50-200
Optimal working dilutions must be determined by end user.
Section Bar
 Western Blot (WB) of anti-COL4a5 antibody    (Figure. Western Blot; Sample: Recombinant COL4a5, Human.)
anti-COL4a5 antibody Western Blot (WB) (WB) image
Table BarTOPTable Bar
NCBI/Uniprot data below describe general gene information for COL4a5. It may not necessarily be applicable to this product.
 NCBI GI #    4502955
 NCBI GeneID    1287
 NCBI Accession #    NP_000486.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_000495.4 [Other Products]
Section Bar
 UniProt Primary Accession #    P29400 [Other Products]
 UniProt Secondary Accession #    Q16006; Q16126; Q6LD84; Q7Z700; Q9NUB7 [Other Products]
 UniProt Related Accession #    P29400 [Other Products]
Table BarTOPTable Bar
 NCBI Official Full Name    collagen alpha-5(IV) chain isoform 1
 NCBI Official Synonym Full Names    collagen type IV alpha 5 chain
 NCBI Official Symbol    COL4A5 [Similar Products]
 NCBI Official Synonym Symbols   
ATS; ASLN; CA54
[Similar Products]
 NCBI Protein Information    collagen alpha-5(IV) chain
 UniProt Protein Name    Collagen alpha-5(IV) chain
 Protein Family    Collagen
 UniProt Gene Name    COL4A5 [Similar Products]
Section Bar
 NCBI Summary for COL4a5    This gene encodes one of the six subunits of type IV collagen, the major structural component of basement membranes. Mutations in this gene are associated with X-linked Alport syndrome, also known as hereditary nephritis. Like the other members of the type IV collagen gene family, this gene is organized in a head-to-head conformation with another type IV collagen gene so that each gene pair shares a common promoter. Alternatively spliced transcript variants have been identified for this gene. [provided by RefSeq, Aug 2010]
Section Bar
 UniProt Comments for COL4a5    COL4A5: Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen. Defects in COL4A5 are the cause of Alport syndrome X- linked (APSX). APSX is characterized by progressive glomerulonephritis, renal failure, sensorineural deafness, specific eye abnormalities (lenticonous and macular flecks), and glomerular basement membrane defects. The disorder shows considerable heterogeneity in that families differ in the age of end-stage renal disease and the occurrence of deafness. Deletions covering the N-terminal regions of COL4A5 and COL4A6, which are localized in a head-to-head manner, are found in the chromosome Xq22.3 centromeric deletion syndrome. This results in a phenotype with features of diffuse leiomyomatosis and Alport syndrome (DL-ATS). Belongs to the type IV collagen family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Extracellular matrix; Motility/polarity/chemotaxis; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: Xq22.3

Cellular Component: basal lamina; collagen type IV; endoplasmic reticulum lumen; extracellular region; neuromuscular junction

Molecular Function: extracellular matrix structural constituent

Biological Process: collagen catabolic process; extracellular matrix organization; neuromuscular junction development

Disease: Alport Syndrome, X-linked
Table BarTOPTable Bar
 Research Articles on COL4a5    1. variant c.2858G>T, rs78972735, annotated as a pathogenic mutation in dbSNP and human gene mutation database (HGMD), found in four family members with no clinical traits of Alport syndrome
Table BarTOPTable Bar
 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Table BarTOPTable Bar
Pathways associated with anti-COL4a5 antibodyDiseases associated with anti-COL4a5 antibody
 Products by Pathway  Pathway Diagram
 Amoebiasis Pathway antibodies  Amoebiasis Pathway Diagram
 Amoebiasis Pathway antibodies  Amoebiasis Pathway Diagram
 Assembly Of Collagen Fibrils And Other Multimeric Structures Pathway antibodies  Assembly Of Collagen Fibrils And Other Multimeric Structures Pathway Diagram
 Axon Guidance Pathway antibodies  Axon Guidance Pathway Diagram
 Collagen Biosynthesis And Modifying Enzymes Pathway antibodies  Collagen Biosynthesis And Modifying Enzymes Pathway Diagram
 Collagen Formation Pathway antibodies  Collagen Formation Pathway Diagram
 Developmental Biology Pathway antibodies  Developmental Biology Pathway Diagram
 ECM-receptor Interaction Pathway antibodies  ECM-receptor Interaction Pathway Diagram
 ECM-receptor Interaction Pathway antibodies  ECM-receptor Interaction Pathway Diagram
 Extracellular Matrix Organization Pathway antibodies  Extracellular Matrix Organization Pathway Diagram
 Disease Name  Pubmed Publications
 Kidney Diseases Antibodies  >266 publications with COL4a5 and Kidney Diseases
 ALPORT SYNDROME, X-LINKED Antibodies  >251 publications with COL4a5 and ALPORT SYNDROME, X-LINKED
 Proteinuria Antibodies  >41 publications with COL4a5 and Proteinuria
 Neoplasms Antibodies  >33 publications with COL4a5 and Neoplasms
 Eye Diseases Antibodies  >18 publications with COL4a5 and Eye Diseases
 Autoimmune Diseases Antibodies  >9 publications with COL4a5 and Autoimmune Diseases
 Fibrosis Antibodies  >3 publications with COL4a5 and Fibrosis
 Necrosis Antibodies  >3 publications with COL4a5 and Necrosis
 Inflammation Antibodies  >3 publications with COL4a5 and Inflammation
 Hypertrophy Antibodies  >2 publications with COL4a5 and Hypertrophy
Organs/Tissues associated with anti-COL4a5 antibody
 Organ/Tissue Name  Pubmed Publications
 Kidney Antibodies  >153 publications with COL4a5 and Kidney
 Muscle Antibodies  >22 publications with COL4a5 and Muscle
 Eye Antibodies  >22 publications with COL4a5 and Eye
 Ear Antibodies  >10 publications with COL4a5 and Ear
 Vascular Antibodies  >9 publications with COL4a5 and Vascular
 Bone Antibodies  >6 publications with COL4a5 and Bone
 Embryonic Tissue Antibodies  >5 publications with COL4a5 and Embryonic Tissue
 Brain Antibodies  >4 publications with COL4a5 and Brain
 Liver Antibodies  >2 publications with COL4a5 and Liver
 Bladder Antibodies  >2 publications with COL4a5 and Bladder
Table BarTOPTable Bar
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions