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anti-CYCS antibody :: Mouse Cytochrome C Monoclonal Antibody

Scan QR to view Datasheet Catalog #    MBS439344
Unit / Price
0.02 mg (With BSA & Azide at 0.2mg/ml)  /  $190 +1 FREE 8GB USB
7 ml (Prediluted)  /  $270 +1 FREE 8GB USB
0.1 mg (With BSA & Azide at 0.2mg/ml)  /  $300 +1 FREE 8GB USB
0.1 mg (Without BSA & Azide at 1mg/ml)  /  $310 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Cytochrome C (CYCS), Monoclonal Antibody

★Popular Item★
 Also Known As   

Cytochrome C (Mitochondrial Marker) Mouse Monoclonal Antibody

 Product Synonym Names    CYC; CYCS; HCS; THC4
 Product Gene Name   

anti-CYCS antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 OMIM    123970
 3D Structure    ModBase 3D Structure for P99999
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 Clonality    Monoclonal
 Isotype    IgG2b, kappa
 Clone Number    7H8.2C12
 Host    Mouse
 Species Reactivity    Human, Mouse, Rat, Horse, Dog, Pigeon, Frog, Drosophila.
Others not known
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 Specificity    It recognizes an epitope within amino acids 93-104 of pigeon Cytochrome C, a well-characterized mobile electron transport protein that is essential to energy conversion in all aerobic organisms. In mammalian cells, this highly conserved protein is normally localized to the mitochondrial inter-membrane space. More recent studies have identified cytosolic cytochrome c as a factor necessary for activation of apoptosis. During apoptosis, cytochrome c is trans-located from the mitochondrial membrane to the cytosol, where it is required for activation of caspase-3 (CPP32). Overexpression of Bcl-2 has been shown to prevent the translocation of cytochrome c, thereby blocking the apoptotic process. Overexpression of Bax has been shown to induce the release of cytochrome c and to induce cell death. The release of cytochrome c from the mitochondria is thought to trigger an apoptotic cascade, whereby Apaf-1 binds to Apaf-3 (caspase-9) in a cytochrome c-dependent manner, leading to caspase-9 cleavage of caspase-3. This MAb recognizes total cytochrome C which includes both apocytochrome (i.e. cytochrome in the cytosol without heme attached) and holocytochrome (i.e cytochrome in the mitochondria with heme attached).
 Form/Format    200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 1mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
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 Cellular Localization    Cytoplasmic
 Immunogen    Synthetic peptides corresponding to amino acid 1-80, 81-104 and 66-104 of pigeon cytochrome c
 Hu-Chromosome Location    7p15.3
 Positive Control    K-562, HL-60, Jurkat, NIH3T3 or PC-3 cells. Liver or Cardiac muscle.
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 Preparation and Storage    Antibody with azide - store at 2 to 8 degree C. Antibody without azide - store at -20 to -80 degree C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
 Other Notes    Small volumes of anti-CYCS antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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 Applications Tested/Suitable for anti-CYCS antibody   

Flow Cytometry (FC/FACS), Immunofluorescence (IF), Western Blot (WB), Immunohistochemistry (IHC) Formalin

 Application Notes for anti-CYCS antibody    Flow Cytometry (0.5-1ug/million cells in 0.1ml)
Immunofluorescence (0.5-1ug/ml)
Western Blot (0.5-1ug/ml)
Immunohistochemistry (Formalin-fixed) (0.25-0.5ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
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 Immunohistochemistry (IHC) of anti-CYCS antibody    Formalin-fixed, paraffin-embedded human Salivary Tumor stained with Cytochrome C Monoclonal Antibody (7H8.2C12).
anti-CYCS antibody Immunohistochemistry (IHC) (IHC) image
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 Immunohistochemistry (IHC) of anti-CYCS antibody    Formalin-fixed, paraffin-embedded human Pancreas stained with Cytochrome C Monoclonal Antibody (7H8.2C12).
anti-CYCS antibody Immunohistochemistry (IHC) (IHC) image
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NCBI/Uniprot data below describe general gene information for CYCS. It may not necessarily be applicable to this product.
 NCBI GI #    11128019
 NCBI GeneID    54205
 NCBI Accession #    NP_061820.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_018947.5 [Other Products]
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 UniProt Primary Accession #    P99999 [Other Products]
 UniProt Secondary Accession #    P00001; Q6NUR2; Q6NX69; Q96BV4; A4D166; B2R4I1 [Other Products]
 UniProt Related Accession #    P99999 [Other Products]
 Molecular Weight    15kDa
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 NCBI Official Full Name    cytochrome c
 NCBI Official Synonym Full Names    cytochrome c, somatic
 NCBI Official Symbol    CYCS [Similar Products]
 NCBI Official Synonym Symbols   
CYC; HCS; THC4
[Similar Products]
 NCBI Protein Information    cytochrome c
 UniProt Protein Name    Cytochrome c
 Protein Family    Cytochrome
 UniProt Gene Name    CYCS [Similar Products]
 UniProt Synonym Gene Names    CYC [Similar Products]
 UniProt Entry Name    CYC_HUMAN
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 NCBI Summary for CYCS    This gene encodes a small heme protein that functions as a central component of the electron transport chain in mitochondria. The encoded protein associates with the inner membrane of the mitochondrion where it accepts electrons from cytochrome b and transfers them to the cytochrome oxidase complex. This protein is also involved in initiation of apoptosis. Mutations in this gene are associated with autosomal dominant nonsyndromic thrombocytopenia. Numerous processed pseudogenes of this gene are found throughout the human genome.[provided by RefSeq, Jul 2010]
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 UniProt Comments for CYCS    CYCS: Electron carrier protein. The oxidized form of the cytochrome c heme group can accept an electron from the heme group of the cytochrome c1 subunit of cytochrome reductase. Cytochrome c then transfers this electron to the cytochrome oxidase complex, the final protein carrier in the mitochondrial electron-transport chain. Belongs to the cytochrome c family.

Protein type: Mitochondrial; Oxidoreductase; Protein phosphatase, Ser/Thr (non-receptor); Apoptosis

Chromosomal Location of Human Ortholog: 7p15.3

Cellular Component: cytosol; mitochondrial inner membrane; mitochondrial intermembrane space; mitochondrion; nucleus; protein phosphatase type 2A complex

Molecular Function: electron transporter, transferring electrons from CoQH2-cytochrome c reductase complex and cytochrome c oxidase complex activity; heme binding; protein binding; protein serine/threonine phosphatase activity

Biological Process: caspase activation via cytochrome c; cellular respiration; mitochondrial electron transport, cytochrome c to oxygen; mitochondrial electron transport, ubiquinol to cytochrome c; mitochondrion organization and biogenesis; response to reactive oxygen species

Disease: Thrombocytopenia 4
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Product References and Citations for anti-CYCS antibody

   Goshorn SG, E Retzel, and R Jemmerson. Common Structural Features among Monoclonal Antibodies Binding the Same Antigenic Region of Cytochrome c. J Biol Chem 266:2134-2142 (1991).
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 Research Articles on CYCS    1. These findings establish a framework for understanding the molecular basis of cytochrome c-mediated blocking of SET/TAF-Ibeta.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with anti-CYCS antibodyDiseases associated with anti-CYCS antibody
 Products by Pathway  Pathway Diagram
 AGE/RAGE Pathway antibodies  AGE/RAGE Pathway Diagram
 Activation Of Caspases Through Apoptosome-mediated Cleavage Pathway antibodies  Activation Of Caspases Through Apoptosome-mediated Cleavage Pathway Diagram
 Alzheimer's Disease Pathway antibodies  Alzheimer's Disease Pathway Diagram
 Alzheimer's Disease Pathway antibodies  Alzheimer's Disease Pathway Diagram
 Alzheimers Disease Pathway antibodies  Alzheimers Disease Pathway Diagram
 Amyotrophic Lateral Sclerosis (ALS) Pathway antibodies  Amyotrophic Lateral Sclerosis (ALS) Pathway Diagram
 Amyotrophic Lateral Sclerosis (ALS) Pathway antibodies  Amyotrophic Lateral Sclerosis (ALS) Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
 Disease Name  Pubmed Publications
 Thrombocytopenia Antibodies  >6 publications with CYCS and Thrombocytopenia
 Necrosis Antibodies  >2 publications with CYCS and Necrosis
 Liver Diseases Antibodies  >1 publications with CYCS and Liver Diseases
 Hyperglycemia Antibodies  >1 publications with CYCS and Hyperglycemia
 Myocardial Infarction Antibodies  >1 publications with CYCS and Myocardial Infarction
 Neoplasms, Experimental Antibodies  >1 publications with CYCS and Neoplasms, Experimental
 Fibrosis Antibodies  >1 publications with CYCS and Fibrosis
 Heart Diseases Antibodies  >1 publications with CYCS and Heart Diseases
 Thrombocytopenia 4 Antibodies  >1 publications with CYCS and Thrombocytopenia 4
 Neoplasm Metastasis Antibodies  >1 publications with CYCS and Neoplasm Metastasis
Organs/Tissues associated with anti-CYCS antibody
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >7 publications with CYCS and Blood
 Prostate Antibodies  >5 publications with CYCS and Prostate
 Muscle Antibodies  >5 publications with CYCS and Muscle
 Liver Antibodies  >4 publications with CYCS and Liver
 Lung Antibodies  >3 publications with CYCS and Lung
 Heart Antibodies  >3 publications with CYCS and Heart
 Brain Antibodies  >3 publications with CYCS and Brain
 Vascular Antibodies  >2 publications with CYCS and Vascular
 Bone Antibodies  >2 publications with CYCS and Bone
 Skin Antibodies  >1 publications with CYCS and Skin
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