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anti-KRT8 antibody :: Mouse CytokeRatin 8 / KeRatin K8 Monoclonal Antibody

Scan QR to view Datasheet Catalog #    MBS570004
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 Product Name   

CytokeRatin 8 / KeRatin K8 (KRT8), Monoclonal Antibody

★Popular Item★
 Also Known As   

Mouse anti CytokeRatin 8 / KeRatin K8

 Product Gene Name   

anti-KRT8 antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Chromosome Location    Chromosome: 12; NC_000012.11 (53290971..53298868, complement). Location: 12q13
 OMIM    148060
 3D Structure    ModBase 3D Structure for P05787
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 Clonality    Monoclonal
 Isotype    IgG1
 Clone Number    M20
 Host    Mouse
 Species Reactivity    Human, Rabbit, Rat
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 Specificity    M20 reacts with glandular epithelial cells (endocrine and exocrine) as well as mesothelial cells and epithelia of the digestive, respiRatory and urogenital tract. Also most adenocarcinomas derived from these cells are positive. The M20 epitope has been mapped to amino acids 353-367 on the alpha helical rod domain of cytokeRatin 8 (see reference 10).
 Form/Format    Each vial contains 1ml of culture supernatant of monoclonal antibody containing 0.09% sodium azide.
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 Source Note    M20 is a Mouse monoclonal IgG1 antibody derived by fusion of murine myeloma cells with spleen cells from a Mouse immunized with keRatin isolated from the Human breast carcinoma cell line MCF-7.
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 Preparation and Storage    Store at 4 degree C, or in small aliquots at -20 degree C.
 Other Notes    Small volumes of anti-KRT8 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for anti-KRT8 antibody

   CytokeRatins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40 kDa and 68 kDa and isoelectric pH between 4.9 - 7.8. The individual cytokeRatin polypeptides are numbered 1 to 20. The various epithelia in the Human body usually express cytokeRatins which are not only characteristic of the type of epithelium, but also related to the degree of matuRation or differentiation within an epithelium. CytokeRatin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeRatin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.
 Product Categories/Family for anti-KRT8 antibody    Cytoskeletal, Cell differentiation, Cytoskeleton
 Applications Tested/Suitable for anti-KRT8 antibody   

Flow Cytometry (FC), Immunocytochemistry (IHC), Immunohistochemistry (IHC) (frozen), Immunohistochemistry (IHC) (paraffin), Immunoblotting

 Application Notes for anti-KRT8 antibody    M20 is suitable for immunoblotting, immunocytochemistry, immunohistochemistry on frozen and paraffin-embedded tissues and flow cytometry. Optimal antibody dilution should be determined by titRation; recommended range is 1:2 - 1:10 for immunohistochemistry with avidin-biotinylated Horseradish peroxidase complex (ABC) as detection reagent, and 1:5 - 1:50 for immunoblotting appliCations.
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 Testing Data of anti-KRT8 antibody    Figure 1: Immunohistochemistry on frozen section of human colon
anti-KRT8 antibody Testing Data image
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 Testing Data of anti-KRT8 antibody    Figure 2: Immunohistochemistry on paraffin section of human colon
anti-KRT8 antibody Testing Data image
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NCBI/Uniprot data below describe general gene information for KRT8. It may not necessarily be applicable to this product.
 NCBI GI #    181400
 NCBI GeneID    3856
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 UniProt Primary Accession #    P05787 [Other Products]
 UniProt Secondary Accession #    Q14099; Q14716; Q14717; Q53GJ0; Q6DHW5; Q6GMY0; Q6P4C7; Q96J60; A8K4H3; B0AZN5 [Other Products]
 UniProt Related Accession #    P05787; Q7L4M3; Q969I0 [Other Products]
 Molecular Weight    53,704 Da [Similar Products]
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 NCBI Official Full Name    cytokeratin 8
 NCBI Official Synonym Full Names    keratin 8
 NCBI Official Symbol    KRT8 [Similar Products]
 NCBI Official Synonym Symbols   
K8; KO; CK8; CYK8; K2C8; CARD2
[Similar Products]
 NCBI Protein Information    keratin, type II cytoskeletal 8; CK-8; keratin-8; cytokeratin 8; cytokeratin-8; type-II keratin Kb8
 UniProt Protein Name    Keratin, type II cytoskeletal 8
 UniProt Synonym Protein Names   
Cytokeratin-8; CK-8; Keratin-8; K8; Type-II keratin Kb8
 Protein Family    Keratin
 UniProt Gene Name    KRT8 [Similar Products]
 UniProt Synonym Gene Names    CYK8 [Similar Products]
 UniProt Entry Name    K2C8_HUMAN
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 NCBI Summary for KRT8    This gene is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. The product of this gene typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis. [provided by RefSeq]
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 UniProt Comments for KRT8    K8: a type II cytoskeletal keratin. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. Phosphorylation of keratins at specific sites affects their organization, assembly dynamics, and their interaction with signaling molecules. Phsophorylated by p38 kinase, regulating cellular keratin filament reorganization. Phosphorylation on serine residues is enhanced during EGF stimulation and mitosis. Mutation of this protein is a risk factor for cryptogenic liver failure.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 12q13

Cellular Component: dystrophin-associated glycoprotein complex; nucleoplasm; intermediate filament cytoskeleton; nuclear matrix; costamere; keratin filament; cytoplasm; intermediate filament; intercellular junction; nucleus; Z disc; sarcolemma

Molecular Function: protein binding; protein complex binding; structural molecule activity

Biological Process: tumor necrosis factor-mediated signaling pathway; viral reproduction; sarcomere organization; response to other organism; response to hydrostatic pressure

Disease: Cirrhosis, Familial
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Product References and Citations for anti-KRT8 antibody

   Van Muijen, G. N., Ruiter, D. J., and Warnaar, S. O. (1987). Coexpression of intermediate filament polypeptides in Human fetal and adult tissues. Lab Invest 57, 359-69. Van Muijen, G. N., Warnaar, S. O., and Ponec, M. (1987). Differentiation-related changes of cytokeRatin expression in cultured keRatinocytes and in fetal, newborn, and adult epidermis. Exp Cell Res 171, 331-45. Schaafsma, H. E., Ramaekers, F. C., van Muijen, G. N., Ooms, E. C., and Ruiter, D. J. (1989). Distribution of cytokeRatin polypeptides in epithelia of the adult Human urinary tract. Histochemistry 91, 151-9. Smedts, F., Ramaekers, F., Robben, H., Pruszczynski, M., van Muijen, G., Lane, B., Leigh, I., and Vooijs, P. (1990). Changing patterns of keRatin expression during progression of cervical intraepithelial neoplasia. Am J Pathol 136, 657-68. Ramaekers, F., van Niekerk, C., Poels, L., Schaafsma, E., Huijsmans, A., Robben, H., Schaart, G., and Vooijs, P. (1990). Use of monoclonal antibodies to keRatin 7 in the differential diagnosis of adenocarcinomas. Am J Pathol 136, 641-55. Schaafsma, H. E., Ramaekers, F. C., van Muijen, G. N., Lane, E. B., Leigh, I. M., Robben, H., Huijsmans, A., Ooms, E. C., and Ruiter, D. J. (1990). Distribution of cytokeRatin polypeptides in Human transitional cell carcinomas, with special emphasis on changing expression patterns during tumor progression. Am J Pathol 136, 329-43. Ivanyi, D., Groeneveld, E., Van Doornewaard, G., Mooi, W. J., and Hageman, P. C. (1990). KeRatin subtypes in carcinomas of the uterine cervix: impliCations for histogenesis and differential diagnosis. Cancer Res 50, 5143-52. Wetzels, R. H., Kuijpers, H. J., Lane, E. B., Leigh, I. M., Troyanovsky, S. M., Holland, R., van Haelst, U. J., and Ramaekers, F. C. (1991). Basal cell-specific and hyperprolifeRation-related keRatins in Human breast cancer. Am J Pathol 138, 751-63. Ku, N. O., Gish, R., Wright, T. L., and Omary, M. B. (2001). KeRatin 8 mutations in patients with cryptogenic liver disease. N Engl J Med 344, 1580-7. Waseem, A., Karsten, U., Leigh, I.M., Purkis, P., Waseem, H. and Lane, B. (2004). Conformational changes in the rod domain of Human keRatin 8 following heterotypic association with keRatin 18 and its impliCation for filament stability. Biochemistry 43, 1283-95.
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 Research Articles on KRT8    1. The study demonstrates the strong expression of CK8 in limbal epithelial basal cells, which is maintained during the differentiation and migration of the limbal cells toward the central corneal epithelium.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-KRT8 antibodyPathways associated with anti-KRT8 antibody
 Reference Product  PubMed Publications
 KRT18 antibody  >31 publications with KRT8 and KRT18
 EGFR antibody  >5 publications with KRT8 and EGFR
 MAPK1 antibody  >1 publications with KRT8 and MAPK1
 FGFR3 antibody  >1 publications with KRT8 and FGFR3
 Products by Pathway  Pathway Diagram
 EGFR1 Signaling Pathway antibodies  EGFR1 Signaling Pathway Diagram
 Signaling Mediated By P38-alpha And P38-beta Pathway antibodies  Signaling Mediated By P38-alpha And P38-beta Pathway Diagram
Diseases associated with anti-KRT8 antibodyOrgans/Tissues associated with anti-KRT8 antibody
 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >38 publications with KRT8 and Neoplasms
 Carcinoma Antibodies  >28 publications with KRT8 and Carcinoma
 Liver Diseases Antibodies  >26 publications with KRT8 and Liver Diseases
 Skin Diseases Antibodies  >12 publications with KRT8 and Skin Diseases
 Adenocarcinoma Antibodies  >12 publications with KRT8 and Adenocarcinoma
 Intestinal Diseases Antibodies  >11 publications with KRT8 and Intestinal Diseases
 Hepatitis Antibodies  >10 publications with KRT8 and Hepatitis
 Death Antibodies  >8 publications with KRT8 and Death
 Fibrosis Antibodies  >8 publications with KRT8 and Fibrosis
 Liver Cirrhosis Antibodies  >8 publications with KRT8 and Liver Cirrhosis
 Organ/Tissue Name  Pubmed Publications
 Liver Antibodies  >36 publications with KRT8 and Liver
 Kidney Antibodies  >8 publications with KRT8 and Kidney
 Lung Antibodies  >6 publications with KRT8 and Lung
 Eye Antibodies  >5 publications with KRT8 and Eye
 Intestine Antibodies  >5 publications with KRT8 and Intestine
 Prostate Antibodies  >4 publications with KRT8 and Prostate
 Pancreas Antibodies  >4 publications with KRT8 and Pancreas
 Mammary Gland Antibodies  >3 publications with KRT8 and Mammary Gland
 Vascular Antibodies  >3 publications with KRT8 and Vascular
 Placenta Antibodies  >2 publications with KRT8 and Placenta
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