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anti-KRT77 antibody :: Mouse Cytokeratin, pan Monoclonal Antibody

Scan QR to view Datasheet Catalog #    MBS438275
Unit / Price
0.02 mg (With BSA & Azide at 0.2mg/ml)  /  $190 +1 FREE 8GB USB
7 ml (Prediluted)  /  $270 +1 FREE 8GB USB
0.1 mg (With BSA & Azide at 0.2mg/ml)  /  $300 +1 FREE 8GB USB
0.1 mg (Without BSA & Azide at 1mg/ml)  /  $310 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Cytokeratin, pan (KRT77), Monoclonal Antibody

★Popular Item★
 Also Known As   

Cytokeratin, pan (Epithelial Marker) Mouse Monoclonal Antibody

 Product Synonym Names    K1B; KRT1B; Keratin, type II cytoskeletal 1b; K77; CK-1B; Keratin 1B; Keratin-77; Cytokeratin-1B; Type-II Keratin Kb39
 Product Gene Name   

anti-KRT77 antibody

[Similar Products]
 Product Synonym Gene Name    KRT76 [Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 OMIM    611158
 3D Structure    ModBase 3D Structure for Q7Z794
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 Clonality    Monoclonal
 Isotype    IgG
 Clone Number    AE-1/AE-3
 Host    Mouse
 Species Reactivity    Human, Monkey, Cow, Dog, Rabbit, Mouse, Rat, Chicken
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 Specificity    Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. This antibody cocktail recognizes acidic (Type I or LMW) and basic (Type II or HMW) cytokeratins, which 67kDa (CK1); 64kDa (CK3); 59kDa (CK4); 58kDa (CK5); 56kDa (CK6); 52kDa (CK8); 56.5kDa (CK10); 50kDa (CK14); 50kDa (CK15); 48kDa (CK16); 40kDa (CK19). Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis. AE-1/AE-3 is a broad spectrum anti pan-cytokeratin antibody cocktail, which differentiates epithelial tumors from non-epithelial tumors e.g. squamous vs. adenocarcinoma of the lung, liver carcinoma, breast cancer, and esophageal cancer. It has been used to characterize the source of various neoplasms and to study the distribution of cytokeratin containing cells in epithelia during normal development and during the development of epithelial neoplasms. This antibody stains cytokeratins present in normal and abnormal human tissues and has shown high sensitivity in the recognition of epithelial cells and carcinomas.
 Form/Format    200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
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 Cellular Localization    Cytoplasmic
 Immunogen    Human epidermal keratin
 Hu-Chromosome Location    17q21.2 (KRT77) & 12q13.13 (KRT76)
 Positive Control    Skin, Adeno- or Squamous carcinomas
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 Preparation and Storage    Antibody with azide - store at 2 to 8 degree C. Antibody without azide - store at -20 to -80 degree C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
 Other Notes    Small volumes of anti-KRT77 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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 Applications Tested/Suitable for anti-KRT77 antibody   

Flow Cytometry (FC/FACS), Immunofluorescence (IF), Western Blot (WB), Immunohistochemistry (IHC) Formalin

 Application Notes for anti-KRT77 antibody    Flow Cytometry (0.5-1ug/million cells in 0.1ml)
Immunofluorescence (1-2ug/ml)
Western Blot (0.5-1ug/ml for 2 hours at RT)
Immunohistochemistry (Formalin-fixed) (0.25-0.5ug/ml for 30 min at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
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 Immunohistochemistry (IHC) of anti-KRT77 antibody    Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with pan Cytokeratin Monoclonal Antibody cocktail (AE-1/AE3).
anti-KRT77 antibody Immunohistochemistry (IHC) (IHC) image
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 Flow Cytometry (FC/FACS of anti-KRT77 antibody    Flow Cytometry of human Pan-Cytokeratins on HeLa Cells. Black: Cells alone; Green: Isotype Control; Red: PE-labeled Pan-Cytokeratin Monoclonal Antibody (AE-1/AE-3).
anti-KRT77 antibody Flow Cytometry (FC/FACS (FC/FACS) image
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NCBI/Uniprot data below describe general gene information for KRT77. It may not necessarily be applicable to this product.
 NCBI GI #    194018511
 NCBI GeneID    374454
 NCBI Accession #    NP_778253.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_175078.2 [Other Products]
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 UniProt Primary Accession #    Q7Z794 [Other Products]
 UniProt Secondary Accession #    Q7RTS8 [Other Products]
 UniProt Related Accession #    Q7Z794 [Other Products]
 Molecular Weight    40-67kDa
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 NCBI Official Full Name    keratin, type II cytoskeletal 1b
 NCBI Official Synonym Full Names    keratin 77
 NCBI Official Symbol    KRT77 [Similar Products]
 NCBI Official Synonym Symbols   
K1B; KRT1B
[Similar Products]
 NCBI Protein Information    keratin, type II cytoskeletal 1b
 UniProt Protein Name    Keratin, type II cytoskeletal 1b
 UniProt Synonym Protein Names   
Cytokeratin-1B; CK-1B; Keratin-77; K77; Type-II keratin Kb39
 Protein Family    Keratin
 UniProt Gene Name    KRT77 [Similar Products]
 UniProt Synonym Gene Names    KRT1B; CK-1B; K77 [Similar Products]
 UniProt Entry Name    K2C1B_HUMAN
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 NCBI Summary for KRT77    Keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into epithelial keratins and hair keratins. This gene encodes an epithelial keratin that is expressed in the skin and eccrine sweat glands. The type II keratins are clustered in a region of chromosome 12q13.[provided by RefSeq, Jun 2009]
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 UniProt Comments for KRT77    K77: Keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into epithelial keratins and hair keratins. This gene encodes an epithelial keratin that is expressed in the skin and eccrine sweat glands. The type II keratins are clustered in a region of chromosome 12q13.[provided by RefSeq, Jun 2009]

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 12q13.13

Cellular Component: cytoskeleton
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Product References and Citations for anti-KRT77 antibody

   Tseng SCG et. al. Cell 1982; 30361. Woodock-Mitchell J et. al. Journal of Cell Biology 1982;95:580-8
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 Research Articles on KRT77    1. Epithelial keratin, K1b, is exclusively expressed in luminal duct cells of eccrine sweat glands.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Organs/Tissues associated with anti-KRT77 antibody
 Organ/Tissue Name  Pubmed Publications
 Skin Antibodies  >5 publications with KRT77 and Skin
 Uncharacterized Tissue Antibodies  >1 publications with KRT77 and Uncharacterized Tissue
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