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anti-DDX5 antibody :: Mouse anti-Human DDX5 Monoclonal Antibody

Scan QR to view Datasheet Catalog #    MBS120235
Unit / Price
0.1 mg  /  $345 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

DDX5, Monoclonal Antibody

★Popular Item★
 Also Known As   

Mouse monoclonal antibody Anti-Human DDX5

 Product Synonym Names    Homo sapiens DEAD (Asp-Glu-Ala-Asp) box polypeptide 5 (DDX5); p68; HLR1; G17P1; HUMP68; DKFZp686J01190
 Product Gene Name   

anti-DDX5 antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 OMIM    180630
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 Clonality    Monoclonal
 Isotype    IgG1
 Clone Number    2257C3a
 Host    Mouse
 Species Reactivity    Human
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 Form/Format    Mouse monoclonal anti-human DDX5antibody in PBS (3.0 mM KCl, 1.5 mM KH PO , 140 mM NaCl, 8.0 mM Na HPO (pH 7.4)) containing 1% bovine serum albumin (BSA) and 0.05% sodium azide (NaN3)
 Concentration    100 ug/ml (1.0 ml) (lot specific)
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 Preparation    This antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.
 Immunogen    Recombinant
 Sterility    Filtered through a 0.22 um membrane.
 Disposal    This antibody solution contains sodium azide (NaN )as a preservative. There is a potential hazard that NaN3 reacts with copper or lead to produce an explosive compound. For safe disposal, the vial has to be washed thoroughly with water.
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 Preparation and Storage    Store at 2-8 degree C for up to one year. We recommend storing at 20 degree C for long-term storage. Avoid repeat freezing and thawing cycles
 Other Notes    Small volumes of anti-DDX5 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for anti-DDX5 antibody

   DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is a RNA-dependent ATPase, and also a proliferation-associated nuclear antigen, specifically reacting with the simian virus 40 tumor antigen. This gene consists of 13 exons, and alternatively spliced transcripts containing several intron sequences have been detected, but no isoforms encoded by these transcripts have been identified. [NCBI Entrez Gene Summary]
 Applications Tested/Suitable for anti-DDX5 antibody   

Immunocytochemistry (ICC), Immunoprecipitation (IP), Western Blot (WB)

 Application Notes for anti-DDX5 antibody    WB: 0.2-2ug/ml
IP: 100-500ug/sample
ICC: 2-100 ug/ml
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 Quality Control of anti-DDX5 antibody    Western blot analysis of immunized recombinant protein, using anti-DDX5 monoclonal antibody.
anti-DDX5 antibody Quality Control image
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 Quality Control #2 of anti-DDX5 antibody    Arrow indicates the region of immunized recombinant protein carrying 50-200 amino acids.
anti-DDX5 antibody Quality Control #2 image
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 Western Blot (WB) of anti-DDX5 antibody    Detection of DDX5 by Western blot.

Samples: Whole cell lysate from human HeLa (H, 25 ug), mouse NIH3T3 (M, 25 ug) and rat F2408 (R, 25 ug) cells. [Lot No. 2257C3a-1]

Predicted molecular weight: 69 kDa
anti-DDX5 antibody Western Blot (WB) (WB) image
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 Immunoprecipitation (IP) of anti-DDX5 antibody    Immunoprecipitation: RIPA lysate of HeLa cells was incubated with anti-DDX5 mAb. [Lot No. 2257C3a-1]

Predicted molecular weight: 69 kDa
anti-DDX5 antibody Immunoprecipitation (IP) (IP) image
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 Immunocytochemistry (ICC) of anti-DDX5 antibody    Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-DDX5 mAb. [Lot No. 2257C3a-1]
anti-DDX5 antibody Immunocytochemistry (ICC) (ICC) image
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NCBI/Uniprot data below describe general gene information for DDX5. It may not necessarily be applicable to this product.
 NCBI GI #    221139768
 NCBI GeneID    1655
 NCBI Accession #    NP_004387.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_004396 [Other Products]
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 UniProt Secondary Accession #    O75681; Q53Y61; B4DLW8; B5BU21; D3DU32; E7ETL9 [Other Products]
 UniProt Related Accession #    P17844 [Other Products]
 Molecular Weight    60,563 Da
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 NCBI Official Full Name    Homo sapiens DEAD (Asp-Glu-Ala-Asp) box helicase 5 (DDX5), mRNA
 NCBI Official Synonym Full Names    DEAD (Asp-Glu-Ala-Asp) box helicase 5
 NCBI Official Symbol    DDX5 [Similar Products]
 NCBI Official Synonym Symbols   
p68; HLR1; G17P1; HUMP68
[Similar Products]
 NCBI Protein Information    probable ATP-dependent RNA helicase DDX5; DEAD (Asp-Glu-Ala-Asp) box polypeptide 5; DEAD box protein 5; DEAD box-5; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 5 (RNA helicase, 68kD); RNA helicase p68
 UniProt Protein Name    Probable ATP-dependent RNA helicase DDX5
 UniProt Synonym Protein Names   
DEAD box protein 5; RNA helicase p68
 Protein Family    Probable ATP-dependent RNA helicase
 UniProt Gene Name    DDX5 [Similar Products]
 UniProt Entry Name    DDX5_HUMAN
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 NCBI Summary for DDX5    DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is a RNA-dependent ATPase, and also a proliferation-associated nuclear antigen, specifically reacting with the simian virus 40 tumor antigen. This gene consists of 13 exons, and alternatively spliced transcripts containing several intron sequences have been detected, but no isoforms encoded by these transcripts have been identified. [provided by RefSeq, Jul 2008]
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 UniProt Comments for DDX5    DDX5: a putative ATP-dependent RNA helicase. A proliferation-associated nuclear antigen, specifically reacting with the simian virus 40 tumor antigen. The rate of ATP hydrolysis is highly stimulated by single-stranded RNA. Belongs to the DEAD box helicase family. DDX5/DDX17 subfamily. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly.

Protein type: Nucleolus; EC 3.6.4.13; RNA-binding; RNA splicing; Spliceosome; Nuclear receptor co-regulator; Helicase

Chromosomal Location of Human Ortholog: 17q21

Cellular Component: nucleoplasm; membrane; nucleolus; nucleus; ribonucleoprotein complex

Molecular Function: calmodulin binding; protein binding; enzyme binding; androgen receptor binding; transcription coactivator activity; estrogen receptor binding; calcium-dependent protein binding; ATP-dependent RNA helicase activity; ATP binding; RNA helicase activity

Biological Process: nuclear mRNA splicing, via spliceosome; circadian rhythm; transcription, DNA-dependent; regulation of osteoblast differentiation; in utero embryonic development; positive regulation of estrogen receptor signaling pathway; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription from RNA polymerase II promoter; positive regulation of DNA damage response, signal transduction by p53 class mediator; cell growth; regulation of viral genome replication; regulation of alternative nuclear mRNA splicing, via spliceosome
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 Research Articles on DDX5    1. Data indicate that armadillo repeat protein ARVCF interacts with the splicing factors the splicing factor SRSF1 (SF2/ASF), the RNA helicase p68 (DDX5), and the heterogeneous nuclear ribonucleoprotein hnRNP H2.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-DDX5 antibodyPathways associated with anti-DDX5 antibody
 Reference Product  PubMed Publications
 DDX17 antibody  >35 publications with DDX5 and DDX17
 DHX9 antibody  >7 publications with DDX5 and DHX9
 TP53 antibody  >4 publications with DDX5 and TP53
 HNRNPH2 antibody  >1 publications with DDX5 and HNRNPH2
 HNRNPK antibody  >1 publications with DDX5 and HNRNPK
 HNRNPH1 antibody  >1 publications with DDX5 and HNRNPH1
 Products by Pathway  Pathway Diagram
 Direct P53 Effectors Pathway antibodies  Direct P53 Effectors Pathway Diagram
 Proteoglycans In Cancer Pathway antibodies  Proteoglycans In Cancer Pathway Diagram
 Proteoglycans In Cancer Pathway antibodies  Proteoglycans In Cancer Pathway Diagram
 Spliceosome Pathway antibodies  Spliceosome Pathway Diagram
 Spliceosome Pathway antibodies  Spliceosome Pathway Diagram
 Transcriptional Misregulation In Cancer Pathway antibodies  Transcriptional Misregulation In Cancer Pathway Diagram
 Transcriptional Misregulation In Cancer Pathway antibodies  Transcriptional Misregulation In Cancer Pathway Diagram
Diseases associated with anti-DDX5 antibodyOrgans/Tissues associated with anti-DDX5 antibody
 Disease Name  Pubmed Publications
 Breast Neoplasms Antibodies  >13 publications with DDX5 and Breast Neoplasms
 Carcinoma Antibodies  >11 publications with DDX5 and Carcinoma
 Nervous System Diseases Antibodies  >7 publications with DDX5 and Nervous System Diseases
 Adenocarcinoma Antibodies  >5 publications with DDX5 and Adenocarcinoma
 Liver Diseases Antibodies  >4 publications with DDX5 and Liver Diseases
 Carcinoma, Hepatocellular Antibodies  >3 publications with DDX5 and Carcinoma, Hepatocellular
 Necrosis Antibodies  >3 publications with DDX5 and Necrosis
 Neoplasms, Experimental Antibodies  >2 publications with DDX5 and Neoplasms, Experimental
 Brain Diseases Antibodies  >2 publications with DDX5 and Brain Diseases
 Neoplasm Metastasis Antibodies  >2 publications with DDX5 and Neoplasm Metastasis
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >8 publications with DDX5 and Blood
 Bone Antibodies  >7 publications with DDX5 and Bone
 Liver Antibodies  >6 publications with DDX5 and Liver
 Kidney Antibodies  >6 publications with DDX5 and Kidney
 Muscle Antibodies  >6 publications with DDX5 and Muscle
 Prostate Antibodies  >6 publications with DDX5 and Prostate
 Embryonic Tissue Antibodies  >4 publications with DDX5 and Embryonic Tissue
 Vascular Antibodies  >4 publications with DDX5 and Vascular
 Connective Tissue Antibodies  >4 publications with DDX5 and Connective Tissue
 Bone Marrow Antibodies  >3 publications with DDX5 and Bone Marrow
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