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anti-DFFB antibody :: Mouse anti-Human DFFB Monoclonal Antibody

Scan QR to view Datasheet Catalog #    MBS120177
Unit / Price
0.1 mg  /  $345 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

DFFB, Monoclonal Antibody

 Also Known As   

Mouse monoclonal antibody Anti-Human DFFB

 Product Synonym Names    Homo sapiens DNA fragmentation factor, 40kDa, beta polypeptide (caspase-activated DNase) (DFFB), transcript variant 1, mRNA; CAD; CPAN; DFF2;DFF40; DFF-40
 Product Gene Name   

anti-DFFB antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 OMIM    601883
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 Clonality    Monoclonal
 Isotype    IgG1
 Clone Number    2263C6a
 Host    Mouse
 Species Reactivity    Human
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 Concentration    100 ug/ml (1.0 ml) (lot specific)
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 Preparation    This antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.
 Sterility    Filtered through a 0.22 um membrane.
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 Other Notes    Small volumes of anti-DFFB antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for anti-DFFB antibody

   Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene but the biological validity of these variants has not been determined. [NCBI Entrez Gene Summary]
 Applications Tested/Suitable for anti-DFFB antibody   

Dot Blot (DB)

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 Quality Control of anti-DFFB antibody    Western blot analysis of immunized recombinant protein, using anti-DFFB monoclonal antibody.
anti-DFFB antibody Quality Control image
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 Quality Control #2 of anti-DFFB antibody    Arrow indicates the region of immunized recombinant protein carrying 50-200 amino acids.
anti-DFFB antibody Quality Control #2 image
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NCBI/Uniprot data below describe general gene information for DFFB. It may not necessarily be applicable to this product.
 NCBI GI #    544063487
 NCBI GeneID    1677
 NCBI Accession #    NP_004393.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_004402 [Other Products]
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 UniProt Secondary Accession #    O60521; Q5SR22; Q9BYI4; Q9BYI5; Q9BYI6 [Other Products]
 UniProt Related Accession #    O76075 [Other Products]
 Molecular Weight    11,482 Da
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 NCBI Official Full Name    Homo sapiens DNA fragmentation factor, 40kDa, beta polypeptide (caspase-activated DNase) (DFFB), transcript variant 2, mRNA
 NCBI Official Synonym Full Names    DNA fragmentation factor, 40kDa, beta polypeptide (caspase-activated DNase)
 NCBI Official Symbol    DFFB [Similar Products]
 NCBI Official Synonym Symbols   
CAD; CPAN; DFF2; DFF40; DFF-40
[Similar Products]
 NCBI Protein Information    DNA fragmentation factor subunit beta; DNA fragmentation factor 40 kDa subunit; caspase-activated DNase; caspase-activated deoxyribonuclease; caspase-activated nuclease
 UniProt Protein Name    DNA fragmentation factor subunit beta
 UniProt Synonym Protein Names   
Caspase-activated deoxyribonuclease; CAD; Caspase-activated DNase; Caspase-activated nuclease; CPAN; DNA fragmentation factor 40 kDa subunit; DFF-40
 Protein Family    DNA fragmentation factor
 UniProt Gene Name    DFFB [Similar Products]
 UniProt Entry Name    DFFB_HUMAN
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 NCBI Summary for DFFB    Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene but the biological validity of some of these variants has not been determined. [provided by RefSeq, Sep 2013]
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 UniProt Comments for DFFB    DFFB: Nuclease that induces DNA fragmentation and chromatin condensation during apoptosis. Degrades naked DNA and induces apoptotic morphology. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.-.-.-; Deoxyribonuclease; Apoptosis

Chromosomal Location of Human Ortholog: 1p36.3

Cellular Component: nucleoplasm; nuclear chromatin; nucleolus; nucleus; cytosol

Molecular Function: enzyme binding; deoxyribonuclease activity

Biological Process: apoptosis; DNA fragmentation during apoptosis; apoptotic chromosome condensation; cell structure disassembly during apoptosis
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 Research Articles on DFFB    1. DFF40/CAD-independent mechanism driving conformational nuclear changes during caspase-dependent cell death
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-DFFB antibodyPathways associated with anti-DFFB antibody
 Reference Product  PubMed Publications
 CASP3 antibody  >38 publications with DFFB and CASP3
 DFFA antibody  >6 publications with DFFB and DFFA
 DNASE2 antibody  >3 publications with DFFB and DNASE2
 CIDEA antibody  >3 publications with DFFB and CIDEA
 CIDEB antibody  >2 publications with DFFB and CIDEB
 Products by Pathway  Pathway Diagram
 Activation Of DNA Fragmentation Factor Pathway antibodies  Activation Of DNA Fragmentation Factor Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
 Apoptosis Modulation And Signaling Pathway antibodies  Apoptosis Modulation And Signaling Pathway Diagram
 Apoptosis Induced DNA Fragmentation Pathway antibodies  Apoptosis Induced DNA Fragmentation Pathway Diagram
 Apoptotic Execution Phase Pathway antibodies  Apoptotic Execution Phase Pathway Diagram
 Caspase Cascade In Apoptosis Pathway antibodies  Caspase Cascade In Apoptosis Pathway Diagram
 FAS Pathway And Stress Induction Of HSP Regulation antibodies  FAS Pathway And Stress Induction Of HSP Regulation Diagram
Diseases associated with anti-DFFB antibodyOrgans/Tissues associated with anti-DFFB antibody
 Disease Name  Pubmed Publications
 Necrosis Antibodies  >13 publications with DFFB and Necrosis
 Carcinoma Antibodies  >7 publications with DFFB and Carcinoma
 Adenocarcinoma Antibodies  >4 publications with DFFB and Adenocarcinoma
 Immune System Diseases Antibodies  >4 publications with DFFB and Immune System Diseases
 Neoplasms, Experimental Antibodies  >4 publications with DFFB and Neoplasms, Experimental
 Cognition Disorders Antibodies  >3 publications with DFFB and Cognition Disorders
 Cardiovascular Diseases Antibodies  >3 publications with DFFB and Cardiovascular Diseases
 Inflammation Antibodies  >2 publications with DFFB and Inflammation
 Disease Models, Animal Antibodies  >2 publications with DFFB and Disease Models, Animal
 Lung Neoplasms Antibodies  >1 publications with DFFB and Lung Neoplasms
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >9 publications with DFFB and Brain
 Lung Antibodies  >3 publications with DFFB and Lung
 Liver Antibodies  >3 publications with DFFB and Liver
 Bone Antibodies  >2 publications with DFFB and Bone
 Vascular Antibodies  >2 publications with DFFB and Vascular
 Connective Tissue Antibodies  >2 publications with DFFB and Connective Tissue
 Kidney Antibodies  >2 publications with DFFB and Kidney
 Skin Antibodies  >1 publications with DFFB and Skin
 Testis Antibodies  >1 publications with DFFB and Testis
 Bone Marrow Antibodies  >1 publications with DFFB and Bone Marrow
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