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anti-dsDNA antibody :: Mouse anti-Human Double Stranded DNA (dsDNA) Monoclonal Antibody

Scan QR to view Datasheet Catalog #    MBS438957
Unit / Price
0.02 mg (With BSA & Azide at 0.2mg/ml)  /  $190 +1 FREE 8GB USB
7 ml (Prediluted)  /  $270 +1 FREE 8GB USB
0.1 mg (With BSA & Azide at 0.2mg/ml)  /  $300 +1 FREE 8GB USB
0.1 mg (Without BSA & Azide at 1mg/ml)  /  $310 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Double Stranded DNA (dsDNA), Monoclonal Antibody

★Popular Item★
 Also Known As   

Double Stranded DNA (dsDNA) (Nuclear Marker) Mouse Monoclonal Antibody

 Product Gene Name   

anti-dsDNA antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Clonality    Monoclonal
 Isotype    IgG3, kappa
 Clone Number    DSD/958
 Host    Mouse
 Species Reactivity    Human
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 Specificity    This MAb recognizes the double stranded DNA in human cells. It can be used to stain the nuclei in cell or tissue preparations and can be used as a nuclear marker in human cells. This MAb produces a homogeneous staining pattern in the nucleus of normal and malignant cells. Deoxyribonucleic acid (DNA) is a nucleic acid that stores long-term information regarding the development and function of all known living organisms. DNA consists of two long nucleotide polymers, which are composed of four bases, namely adenine, thymine, guanine and cytosine, all of which are flanked by a phosphate-deoxyribose backbone. Normally, DNA exists as a double-stranded (ds) molecule that forms in the shape of a double helix, allowing the bases and the backbone of the two strands to interact, thus forming a polynucleotide. When the double helix is unwound (either by enzymes or heat), DNA exists as a single-stranded (ss) molecule that is less stable than the double helix, but is necessary for protein access to DNA bases. Double stranded DNA markers are useful tools in biology research and aid in the study of DNA behavior and characteristics.
 Form/Format    200ug/ml of Ab purified from Bioreactor Concentrate. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
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 Cellular Localization    Nuclear
 Immunogen    Nuclei of Burkitt's cells
 Positive Control    Tonsil
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 Preparation and Storage    Antibody with azide - store at 2 to 8 degree C. Antibody without azide - store at -20 to -80 degree C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
 Other Notes    Small volumes of anti-dsDNA antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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 Applications Tested/Suitable for anti-dsDNA antibody   

Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin

 Application Notes for anti-dsDNA antibody    Flow Cytometry (0.5-1ug/million cells in 0.1ml)
Immunofluorescence (1-2ug/ml)
Immunocytochemistry (Acetone-fixed cells) (0.5-1.0ug/ml for 30 minutes at RT)Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
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 Immunohistochemistry (IHC) of anti-dsDNA antibody    Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with Double Stranded DNA Monoclonal Antibody (DSD/958)
anti-dsDNA antibody Immunohistochemistry (IHC) (IHC) image
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 Immunohistochemistry (IHC) of anti-dsDNA antibody    Formalin-fixed, paraffin-embedded human Tonsil stained with Double Stranded DNA Monoclonal Antibody (DSD/958)
anti-dsDNA antibody Immunohistochemistry (IHC) (IHC) image
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Product References and Citations for anti-dsDNA antibody

   Epstein, A.L. and Clevenger, C.V., Identification of nuclear antigens in human cells by immunofluorescence, immunoelectron microscopy, and immuno-biochemical methods using monoclonal antibodies. In Progress on nonhistone protein research, Vol. 1, Isaac Bekhor, ed., 1985, CRC Press, Boca Raton, FL, pp 117-137
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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