Other Notes
Small volumes of anti-E-Cadherin / Cadherin-1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Cadherins constitute a family of transmembrane glycoproteins involved in Ca2+-dependent cell-cell interactions. The members of this family are differentially expressed in various tissues. They function in the maintenance of tissue integrity and morphogenesis. Cadherins are divided into type I and type II subgroups. Type I cadherins include epithelial cadherin (E-cadherin, cadherin-1 or uvomorulin), neural cadherin (N-cadherin or cadherin-2), placental cadherin (P-cadherin or cadherin-3) and retinal cadherin (R-cadherin or cadherin-4), whereas kidney cadherin (K-cadherin or cadherin-6) and osteoblast cadherin (OB-cadherin or cadherin-11) are type II cadherins. One of the best characterized cadherins is E-cadherin, a 120 kD transmembrane glycoprotein consisting of an 80 kD extracellular and a 40 kD transmembrane and cytoplasmic part. The extracellular domains of E-cadherin are responsible for calcium binding which allows for homophilic interaction with other E-cadherin molecules on the same cell and neighbouring cells. In addition, E-cadherin can interact heterophilically with integrin alphaEbeta7. The cytoplasmic domain of E-cadherin is linked to the actin cytoskeleton through the associated cytoplasmic Catenin proteins, thus establishing a complex localized to adherens junctions. In carcinomas E-cadherin is frequently downregulated, which is consistent with its function of an invasion suppressor in normal epithelia.
5H9 is suitable for immunoblotting, immunocytochemistry and immunohistochemistry on frozen and paraffin embedded tissues. For frozen tissues use a PBS buffer containing 0.1 mM CaCl2 and 0.1 mM MgCl2. For paraffin embedded tissues use a pretreatment step of 15 minutes incubation in TRIS-EDTA buffer pH 9 in a microwave. Optimal antibody dilution should be determined by titRation, recommended range is 1:2 - 1:5 for immunohistochemistry with avidin-biotinylated Horseradish peroxidase complex (ABC) as detection reagent, and 1:2 - 1:25 for immunoblotting appliCations.
Product References and Citations for
anti-E-Cadherin / Cadherin-1 antibody
Frixen, U. H., Behrens, J., Sachs, M., Eberle, G., Voss, B., Warda, A., Lochner, D., and Birchmeier, W. (1991). E-cadherin-mediated cell-cell adhesion prevents invasiveness of Human carcinoma cells, J Cell Biol 113, 173-85. Moll, R., Mitze, M., Frixen, U. H., and Birchmeier, W. (1993). Differential loss of E-cadherin expression in infiltRating ductal and lobular breast carcinomas, Am J Pathol 143, 1731-42. Gabbert, H. E., Mueller, W., Schneiders, A., Meier, S., Moll, R., Birchmeier, W., and Hommel, G. (1996). Prognostic value of E-cadherin expression in 413 gastric carcinomas. Int J Cancer 69, 184-189. Schwechheimer, K., Zhou, L., and Birchmeier, W. (1998). E-Cadherin in Human brain tumours: loss of immunoreactivity in malignant meningiomas. Virchows Arch 432, 163-167.
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