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anti-CDH1 antibody :: Mouse anti-Human E-Cadherin Monoclonal Antibody

Scan QR to view Datasheet Catalog #    MBS690861 anti-CDH1 antibody
Unit / Price
0.1 mg  /  $460 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

E-Cadherin (CDH1), Monoclonal Antibody

 Also Known As   

Mouse Anti-Human E-Cadherin

 Product Synonym Names    CDH1; UVO; CDHE; ECAD; LCAM; Arc-1; CD324
 Product Gene Name   

anti-CDH1 antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Chromosome Location    Chromosome: 16; NC_000016.10 (68737292..68835541). Location: 16q22.1
 OMIM    137215
 3D Structure    ModBase 3D Structure for P12830
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 Clonality    Monoclonal
 Isotype    IgG2
 Clone Number    21E9
 Host    Mouse
 Species Reactivity    Human
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 Form/Format    Lyophilized
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 Antibody Generation    This antibody was produced from a hybridoma (mouse myeloma fused with spleen cells from a mouse immunized with purified recombinant human E-Cadherin. The IgG2 fraction of the tissue culture supernatant was purified by Protein G affinity chromatography.
 Reconstitution    Reconstitute the antibody with 200 ul sterile PBS and the final concentration is 500 ug/ml.
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 Preparation and Storage    Lyophilized samples are stable for 2 years from date of receipt when stored at -70 degree C. Reconstituted antibody can be aliquoted and stored frozen at < -20 degree C for at least for six months without detectable loss of activity.
 ISO Certification    Manufactured in an ISO 9001:2015 Certified Laboratory.
 Other Notes    Small volumes of anti-CDH1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Product Description specifically for anti-CDH1 antibody

   Epithelial (E)Cadherin (E-CAD), also known as Cadherin1, cell-CAM120/ 80 in the human, uvomorulin in the mouse, Arc1 in the dog, and LCAM in the chicken, is a member of the Cadherin family of cell adhesion molecules (gene name CDH1). Cadherins are calcium-dependent transmembrane proteins which bind to one another in a homophilic manner. On their cytoplasmic side, they associate with the three catenins, alpha, beta, and gamma (plakoglobin). This association links the cadherin protein to the cytoskeleton. Without association with the catenins, the cadherins are non-adhesive. Cadherins play a role in development, specifically in tissue formation. They may also help to maintain tissue architecture in the adult. E-Cadherin may also play a role in tumor development, as loss of E-Cadherin has been associated with tumor invasiveness. E-Cadherin is a classical cadherin molecule. Classical cadherins consist of a large extracellular domain which contains DXD and DXNDN repeats responsible for mediating calcium-dependent adhesion, a singlepass-transmembrane domain, and a short carboxyterminal cytoplasmic domain responsible for interacting with the catenins. E-Cadherin contains five extracellular calciumbinding domains of approx. 110 amino acids each (amino acids 155-697).
 Applications Tested/Suitable for anti-CDH1 antibody   

Western Blot (WB), Immunohistochemistry (IHC) - Paraffin

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NCBI/Uniprot data below describe general gene information for CDH1. It may not necessarily be applicable to this product.
 NCBI GI #    4757960
 NCBI GeneID    999
 NCBI Accession #    NP_004351.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_004360.3 [Other Products]
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 UniProt Primary Accession #    P12830 [Other Products]
 UniProt Secondary Accession #    Q13799; Q14216; Q15855; Q16194; Q4PJ14; A8K1U7 [Other Products]
 UniProt Related Accession #    P12830 [Other Products]
 Molecular Weight    97,456 Da [Similar Products]
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 NCBI Official Full Name    cadherin-1 preproprotein
 NCBI Official Synonym Full Names    cadherin 1, type 1, E-cadherin (epithelial)
 NCBI Official Symbol    CDH1 [Similar Products]
 NCBI Official Synonym Symbols   
UVO; CDHE; ECAD; LCAM; Arc-1; CD324
[Similar Products]
 NCBI Protein Information    cadherin-1; CAM 120/80; E-Cadherin; uvomorulin; cell-CAM 120/80; epithelial cadherin; cadherin 1, E-cadherin (epithelial); calcium-dependent adhesion protein, epithelial
 UniProt Protein Name    Cadherin-1
 UniProt Synonym Protein Names   
CAM 120/80; Epithelial cadherin; E-cadherin; Uvomorulin
 Protein Family    Cadherin
 UniProt Gene Name    CDH1 [Similar Products]
 UniProt Synonym Gene Names    CDHE; UVO; E-cadherin [Similar Products]
 UniProt Entry Name    CADH1_HUMAN
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 NCBI Summary for CDH1    This gene is a classical cadherin from the cadherin superfamily. The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Mutations in this gene are correlated with gastric, breast, colorectal, thyroid and ovarian cancer. Loss of function is thought to contribute to progression in cancer by increasing proliferation, invasion, and/or metastasis. The ectodomain of this protein mediates bacterial adhesion to mammalian cells and the cytoplasmic domain is required for internalization. Identified transcript variants arise from mutation at consensus splice sites. [provided by RefSeq, Jul 2008]
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 UniProt Comments for CDH1    Function: Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells. Has a potent invasive suppressor role. It is a ligand for integrin alpha-E/beta-7. Ref.25E-Cad/CTF2 promotes non-amyloidogenic degradation of Abeta precursors. Has a strong inhibitory effect on APP C99 and C83 production. Ref.25

Subunit structure: Homodimer; disulfide-linked. Component of an E-cadherin/ catenin adhesion complex composed of at least E-cadherin/CDH1, beta-catenin/CTNNB1 or gamma-catenin/JUP, and potentially alpha-catenin/CTNNA1; the complex is located to adherens junctions. The stable association of CTNNA1 is controversial as CTNNA1 was shown not to bind to F-actin when assembled in the complex. Alternatively, the CTNNA1-containing complex may be linked to F-actin by other proteins such as LIMA1. Interaction with PSEN1, cleaves CDH1 resulting in the disassociation of cadherin-based adherens junctions (CAJs). Interacts with AJAP1, CTNND1 and DLGAP5

By similarity. Interacts with TBC1D2. Interacts with LIMA1. Interacts with CAV1. Interacts with the TRPV4 and CTNNB1 complex

By similarity. Interacts with PIP5K1C. Interacts with RAB8B

By similarity. Interacts with RAPGEF2

By similarity. Interacts with DDR1; this stabilizes CDH1 at the cell surface and inhibits its internalization. Ref.10 Ref.17 Ref.19 Ref.20 Ref.21 Ref.22 Ref.23 Ref.29 Ref.31 Ref.32

Subcellular location: Cell junction. Cell membrane; Single-pass type I membrane protein. Endosome. Golgi apparatus › trans-Golgi network. Note: Colocalizes with DLGAP5 at sites of cell-cell contact in intestinal epithelial cells. Anchored to actin microfilaments through association with alpha-, beta- and gamma-catenin. Sequential proteolysis induced by apoptosis or calcium influx, results in translocation from sites of cell-cell contact to the cytoplasm. Colocalizes with RAB11A endosomes during its transport from the Golgi apparatus to the plasma membrane. Ref.4 Ref.21 Ref.24 Ref.32

Tissue specificity: Non-neural epithelial tissues.

Induction: Expression is repressed by MACROD1. Ref.26

Domain: Three calcium ions are usually bound at the interface of each cadherin domain and rigidify the connections, imparting a strong curvature to the full-length ectodomain. Ref.16

Post-translational modification: During apoptosis or with calcium influx, cleaved by a membrane-bound metalloproteinase (ADAM10), PS1/gamma-secretase and caspase-3 to produce fragments of about 38 kDa (E-CAD/CTF1), 33 kDa (E-CAD/CTF2) and 29 kDa (E-CAD/CTF3), respectively. Processing by the metalloproteinase, induced by calcium influx, causes disruption of cell-cell adhesion and the subsequent release of beta-catenin into the cytoplasm. The residual membrane-tethered cleavage product is rapidly degraded via an intracellular proteolytic pathway. Cleavage by caspase-3 releases the cytoplasmic tail resulting in disintegration of the actin microfilament system. The gamma-secretase-mediated cleavage promotes disassembly of adherens junctions. Ref.4 Ref.15 Ref.18N-glycosylation at Asn-637 is essential for expression, folding and trafficking.Ubiquitinated by a SCF complex containing SKP2, which requires prior phosphorylation by CK1/CSNK1A1. Ubiquitinated by CBLL1/HAKAI, requires prior phosphorylation at Tyr-754. Ref.34 Ref.35

Involvement in disease: Hereditary diffuse gastric cancer (HDGC) [MIM:137215]: A cancer predisposition syndrome with increased susceptibility to diffuse gastric cancer. Diffuse gastric cancer is a malignant disease characterized by poorly differentiated infiltrating lesions resulting in thickening of the stomach. Malignant tumors start in the stomach, can spread to the esophagus or the small intestine, and can extend through the stomach wall to nearby lymph nodes and organs. It also can metastasize to other parts of the body.Note: The disease is caused by mutations affecting the gene represented in this entry. Heterozygous CDH1 germline mutations are responsible for familial cases of diffuse gastric cancer. Somatic mutations has also been found in patients with sporadic diffuse gastric cancer and lobular breast cancer. Ref.47 Ref.52Endometrial cancer (ENDMC) [MIM:608089]: A malignancy of endometrium, the mucous lining of the uterus. Most endometrial cancers are adenocarcinomas, cancers that begin in cells that make and release mucus and other fluids.Note: Disease susceptibility is associated with variations affecting the gene represented in this entry.Ovarian cancer (OC) [MIM:167000]: The term ovarian cancer defines malignancies originating from ovarian tissue. Although many histologic types of ovarian tumors have been described, epithelial ovarian carcinoma is the most common form. Ovarian cancers are often asymptomatic and the recognized signs and symptoms, even of late-stage disease, are vague. Consequently, most patients are diagnosed with advanced disease.Note: Disease susceptibility is associated with variations affecting the gene represented in this entry.Breast cancer, lobular (LBC) [MIM:137215]: A type of breast cancer that begins in the milk-producing glands (lobules) of the breast.Note: The gene represented in this entry may be involved in disease pathogenesis. Ref.27

Sequence similarities: Contains 5 cadherin domains.

Sequence caution: The sequence AAA61259.1 differs from that shown. Reason: Frameshift at positions 16, 22, 25, 28, 31, 34, 52, 67, 73, 76, 94, 102, 633 and 636.
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 Research Articles on CDH1    1. Our results establish that the E-cadherin ectodomain-specific mAb DECMA-1 inhibits Ecad(+)/HER2(+) breast cancers by hindering tumor growth and inducing apoptosis via downregulation of key oncogenic pathways
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-CDH1 antibodyPathways associated with anti-CDH1 antibody
 Reference Product  PubMed Publications
 CTNNB1 antibody  >130 publications with CDH1 and CTNNB1
 CDC27 antibody  >20 publications with CDH1 and CDC27
 SRC antibody  >9 publications with CDH1 and SRC
 CTNNA1 antibody  >7 publications with CDH1 and CTNNA1
 JUP antibody  >3 publications with CDH1 and JUP
 CBLL1 antibody  >2 publications with CDH1 and CBLL1
 UBC antibody  >1 publications with CDH1 and UBC
 CTNND1 antibody  >1 publications with CDH1 and CTNND1
 Products by Pathway  Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 Adherens Junction Pathway antibodies  Adherens Junction Pathway Diagram
 Adherens Junction Pathway antibodies  Adherens Junction Pathway Diagram
 Adherens Junctions Interactions Pathway antibodies  Adherens Junctions Interactions Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
 Apoptotic Cleavage Of Cell Adhesion Proteins Pathway antibodies  Apoptotic Cleavage Of Cell Adhesion Proteins Pathway Diagram
 Apoptotic Cleavage Of Cellular Proteins Pathway antibodies  Apoptotic Cleavage Of Cellular Proteins Pathway Diagram
 Apoptotic Execution Phase Pathway antibodies  Apoptotic Execution Phase Pathway Diagram
 Arf6 Trafficking Events Pathway antibodies  Arf6 Trafficking Events Pathway Diagram
 Bacterial Invasion Of Epithelial Cells Pathway antibodies  Bacterial Invasion Of Epithelial Cells Pathway Diagram
Diseases associated with anti-CDH1 antibodyOrgans/Tissues associated with anti-CDH1 antibody
 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >913 publications with CDH1 and Neoplasms
 Carcinoma Antibodies  >445 publications with CDH1 and Carcinoma
 Stomach Neoplasms Antibodies  >280 publications with CDH1 and Stomach Neoplasms
 Adenocarcinoma Antibodies  >252 publications with CDH1 and Adenocarcinoma
 Breast Neoplasms Antibodies  >218 publications with CDH1 and Breast Neoplasms
 Neoplasm Metastasis Antibodies  >128 publications with CDH1 and Neoplasm Metastasis
 Neoplasm Invasiveness Antibodies  >116 publications with CDH1 and Neoplasm Invasiveness
 Colorectal Neoplasms Antibodies  >98 publications with CDH1 and Colorectal Neoplasms
 Disease Progression Antibodies  >94 publications with CDH1 and Disease Progression
 Carcinoma, Squamous Cell Antibodies  >64 publications with CDH1 and Carcinoma, Squamous Cell
 Organ/Tissue Name  Pubmed Publications
 Stomach Antibodies  >293 publications with CDH1 and Stomach
 Blood Antibodies  >98 publications with CDH1 and Blood
 Lymph Node Antibodies  >82 publications with CDH1 and Lymph Node
 Lung Antibodies  >78 publications with CDH1 and Lung
 Liver Antibodies  >63 publications with CDH1 and Liver
 Brain Antibodies  >53 publications with CDH1 and Brain
 Prostate Antibodies  >49 publications with CDH1 and Prostate
 Embryonic Tissue Antibodies  >38 publications with CDH1 and Embryonic Tissue
 Kidney Antibodies  >38 publications with CDH1 and Kidney
 Bladder Antibodies  >25 publications with CDH1 and Bladder
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