View our list of available Coronavirus (COVID-19, SARS-CoV-2, 2019-nCOV) antibodies, recombinant proteins, ELISA Kits... Options Full Datasheet Printer Friendly Datasheet Prepare for Printing Full Datasheet Printer Friendly Datasheet Prepare for Printing Have Questions? Ask Us! Add To Compare List E Coli RNA Sigma 70 Monoclonal Antibody Catalog # MBS680120 Unit / Price DISCONTINUEDYou will be redirected to our replacement product Product Name Product Info Accession #s Product Desc Diseases/Tissues/Pathways Applications References Product Name E Coli RNA Sigma 70, Monoclonal Antibody Full Product Name HRP anti-E Coli RNA Sigma 70 Antibody Product Synonym Names RNA pol sigma 70; rpoD; sigma D Research Use Only For Research Use Only. Not for use in diagnostic procedures. TOP Clonality Monoclonal Isotype IgG2b Clone Number 2G10 Species Reactivity E Coli, Chlamydia trachomatis, Mycobacterium smegmatis, Staphylococcus aureus, Bordetella pertussis, Rhodobacter sphaeroides, Vibrio parahaemolyticus, Pseudomonas aeruginosa, Agrobacterium tumefaciens, Streptococcus faecalis, Arthrobacter globiformis Immunogen Recombinant protein Preparation This product is conjugated with horseradish peroxidase under optimal conditions. Structure Sigma 70 (sigma70) is a 70 kD transcription factor of E Coli RNA polymerase. Preparation and Storage Store lyophilized antibody at -20 degree C. After reconstitution, store at 4 degree C. May be sub-aliquoted and stored at -20 degree C to avoid multiple freeze thaw cycles. Other Notes Small volumes of anti-E Coli RNA Sigma 70 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply. TOP Related Product Information for anti-E Coli RNA Sigma 70 antibody RNA polymerase transcribes DNA to synthesize RNA. Prokaryotes contain a single RNA polymerase- core RNA polymerase. RNA polymerase sigma 70 (also known as RNA pol sigma 70 and rpoD) is apart of a family of sigma factor subunits that are required for specific promoter recognition and initiation. RNA pol sigma 70 is the major housekeeping sigma factor responsible for RNA polymerase recognition out of the majority of promoters necessary to transcribe bacterial genes which are expressed during expansion. Applications Tested/Suitable for anti-E Coli RNA Sigma 70 antibody Western Blot (WB), ELISA (EIA), Functional Assay, Immunoprecipitation (IP) Application Notes for anti-E Coli RNA Sigma 70 antibody Each lot of this antibody is quality control tested by Western blotting. To reconstitute, add 50 ul of ddH2O to the tube containing antibody and mix to dissolve. For Western blotting, the suggested dilution is to be determined empirically after reconstitution. It is recommended that the reagent be titrated for optimal performance for each application.Clone 2G10 is extremely useful for identifying the major sigma factor from a variety of bacteria. It does not react with Borellia burgdorferi. Clone 2G10 recognizes epitope 470-486. TOP NCBI GeneID 947567 TOP Product References and Citations for anti-E Coli RNA Sigma 70 antibody 1. Breyer MJ, et al. 1997. J. Bacterial 179:1404.2. Lesley SA, et al. 1989. J. Biol. Chem. 264:3794.3. Strickland MS and Thompson NE and Burgess RR. 1988. Biochemistry 27:5755. TOP Precautions All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines. Disclaimer While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information. TOP