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anti-E2F1 antibody :: Mouse E2F-1 Monoclonal Antibody

Scan QR to view Datasheet Catalog #    MBS190137 anti-E2F1 antibody
Unit / Price
0.1 mL  /  $410 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

E2F-1 (E2F1), Monoclonal Antibody

 Also Known As   

E2F-1 Monoclonal Antibody

 Product Synonym Names    Anti-E2F-1; E2F-1
 Product Gene Name   

anti-E2F1 antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Chromosome Location    Chromosome: 20; NC_000020.10 (32263292..32274210, complement). Location: 20q11.2
 OMIM    189971
 3D Structure    ModBase 3D Structure for Q01094
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 Clonality    Monoclonal
 Isotype    IgG1 kappa
 Host    Mouse
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 Specificity    Anti-E2F-1 [Murine MAb to GST fusion protein corresponding to aa 244-264 of E2F-1, a cellular protein with DNA binding activity associated with the adenovirus E2 gene promoter.]
This antibody recognizes an epitope within aa 244-264 of human E2F-1.
 Form/Format    100 ug Protein G-purified antibody in PBS, pH 7.4
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 Antigen    GST fusion protein expressed in E. coli corresponding to aa 162-476 of human E2F-1.
 Stabilizer    None
 Preservatives    None. Available on request.
 Dilutions Instructions    Dilute in PBS or medium which is identical to that used in the assay system.
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 Preparation and Storage    This antibody is stable for at least one (1) year at -70 degree C. Avoid multiple freeze thaw cycles.
 Other Notes    Small volumes of anti-E2F1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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 Product Categories/Family for anti-E2F1 antibody    Cancer Research Products
 Applications Tested/Suitable for anti-E2F1 antibody   

Immunoblot

 Application Notes for anti-E2F1 antibody    Immunoblotting: use at 1-5 ug/ml.
Immuno precipitation: use at 1-5 ug/ml.
Positive controls: E2F-1 transfectedSoas2 cells and recombinant fusion protein.
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NCBI/Uniprot data below describe general gene information for E2F1. It may not necessarily be applicable to this product.
 NCBI GI #    181918
 NCBI GeneID    1869
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 UniProt Primary Accession #    Q01094 [Other Products]
 UniProt Secondary Accession #    Q13143; Q92768 [Other Products]
 UniProt Related Accession #    Q01094; Q9BSD8 [Other Products]
 Molecular Weight    46,920 Da [Similar Products]
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 NCBI Official Full Name    E2F-1
 NCBI Official Synonym Full Names    E2F transcription factor 1
 NCBI Official Symbol    E2F1 [Similar Products]
 NCBI Official Synonym Symbols   
RBP3; E2F-1; RBAP1; RBBP3
[Similar Products]
 NCBI Protein Information    transcription factor E2F1; PBR3; RBAP-1; RBBP-3; OTTHUMP00000030661; PRB-binding protein E2F-1; retinoblastoma-binding protein 3; retinoblastoma-associated protein 1
 UniProt Protein Name    Transcription factor E2F1
 UniProt Synonym Protein Names   
PBR3; PRB-binding protein E2F-1; Retinoblastoma-associated protein 1; RBAP-1; Retinoblastoma-binding protein 3
 Protein Family    Transcription factor
 UniProt Gene Name    E2F1 [Similar Products]
 UniProt Synonym Gene Names    RBBP3 [Similar Products]
 UniProt Entry Name    E2F1_HUMAN
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 NCBI Summary for E2F1    The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F2 and E2F3, have an additional cyclin binding domain. This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent/independent apoptosis. [provided by RefSeq]
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 UniProt Comments for E2F1    E2F1: a member of the E2F/DP family of transcription factors.The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. Binds DNA cooperatively with Dp transcription factors in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication.The Dp-1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. The E2F-1 complex binds specifically hypophosphorylated retinoblastoma protein RB1. During the cell cycle, RB1 becomes phosphorylated in mid-to-late G1 phase, detaches from the DRTF1/E2F complex, rendering E2F transcriptionally active. Phosphorylated by CDK2 and cyclin A-CDK2 in the S-phase. It can mediate both cell proliferation and p53-dependent apoptosis.

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 20q11.2

Cellular Component: nucleoplasm; Rb-E2F complex; cytoplasm; nucleus

Molecular Function: protein binding; DNA binding; sequence-specific DNA binding; transcription factor binding; transcription factor activity

Biological Process: Notch signaling pathway; transcription, DNA-dependent; apoptosis; positive regulation of transcription, DNA-dependent; mRNA stabilization; negative regulation of transcription from RNA polymerase II promoter; anoikis; positive regulation of fibroblast proliferation; regulation of transcription, DNA-dependent; DNA damage response, signal transduction resulting in induction of apoptosis; DNA damage checkpoint; forebrain development; positive regulation of transcription from RNA polymerase II promoter; spermatogenesis; mitotic cell cycle; negative regulation of transcription, DNA-dependent; G1/S transition of mitotic cell cycle
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 Research Articles on E2F1    1. Findings do not provide support for the model that protein-protein interactions are involved in recruiting E2F1 to the genome, but rather suggest that recognition of a motif found at most human promoters is the critical determinant.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-E2F1 antibodyPathways associated with anti-E2F1 antibody
 Reference Product  PubMed Publications
 MYC antibody  >211 publications with E2F1 and MYC
 E2F4 antibody  >185 publications with E2F1 and E2F4
 E2F2 antibody  >152 publications with E2F1 and E2F2
 CDKN2A antibody  >131 publications with E2F1 and CDKN2A
 TFDP1 antibody  >88 publications with E2F1 and TFDP1
 SP1 antibody  >76 publications with E2F1 and SP1
 ATM antibody  >48 publications with E2F1 and ATM
 RBL1 antibody  >37 publications with E2F1 and RBL1
 RBL2 antibody  >36 publications with E2F1 and RBL2
 CDC25A antibody  >34 publications with E2F1 and CDC25A
 Products by Pathway  Pathway Diagram
 Activation Of BH3-only Proteins Pathway antibodies  Activation Of BH3-only Proteins Pathway Diagram
 Activation Of NOXA And Translocation To Mitochondria Pathway antibodies  Activation Of NOXA And Translocation To Mitochondria Pathway Diagram
 Activation Of PUMA And Translocation To Mitochondria Pathway antibodies  Activation Of PUMA And Translocation To Mitochondria Pathway Diagram
 Adipogenesis Pathway antibodies  Adipogenesis Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
 Assembly Of The Pre-replicative Complex Pathway antibodies  Assembly Of The Pre-replicative Complex Pathway Diagram
 Association Of Licensing Factors With The Pre-replicative Complex Pathway antibodies  Association Of Licensing Factors With The Pre-replicative Complex Pathway Diagram
 Bladder Cancer Pathway antibodies  Bladder Cancer Pathway Diagram
 Bladder Cancer Pathway antibodies  Bladder Cancer Pathway Diagram
 CDC6 Association With The ORC:origin Complex Pathway antibodies  CDC6 Association With The ORC:origin Complex Pathway Diagram
Diseases associated with anti-E2F1 antibodyOrgans/Tissues associated with anti-E2F1 antibody
 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >808 publications with E2F1 and Neoplasms
 Carcinoma Antibodies  >263 publications with E2F1 and Carcinoma
 Skin Diseases Antibodies  >123 publications with E2F1 and Skin Diseases
 Urogenital Neoplasms Antibodies  >122 publications with E2F1 and Urogenital Neoplasms
 Breast Neoplasms Antibodies  >110 publications with E2F1 and Breast Neoplasms
 Cell Transformation, Neoplastic Antibodies  >106 publications with E2F1 and Cell Transformation, Neoplastic
 Adenocarcinoma Antibodies  >100 publications with E2F1 and Adenocarcinoma
 Neoplasms, Experimental Antibodies  >95 publications with E2F1 and Neoplasms, Experimental
 Leukemia Antibodies  >75 publications with E2F1 and Leukemia
 Prostatic Neoplasms Antibodies  >62 publications with E2F1 and Prostatic Neoplasms
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >115 publications with E2F1 and Blood
 Brain Antibodies  >108 publications with E2F1 and Brain
 Lung Antibodies  >106 publications with E2F1 and Lung
 Muscle Antibodies  >83 publications with E2F1 and Muscle
 Liver Antibodies  >81 publications with E2F1 and Liver
 Prostate Antibodies  >71 publications with E2F1 and Prostate
 Skin Antibodies  >61 publications with E2F1 and Skin
 Bone Antibodies  >50 publications with E2F1 and Bone
 Embryonic Tissue Antibodies  >45 publications with E2F1 and Embryonic Tissue
 Eye Antibodies  >39 publications with E2F1 and Eye
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