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anti-EGF antibody :: Mouse EGF Receptor Monoclonal Antibody

Scan QR to view Datasheet Catalog #    MBS475155
Unit / Price
0.1 mL  /  $335 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

EGF Receptor, Monoclonal Antibody

★Popular Item★
 Also Known As   

Anti-EGF Receptor Mouse mAb

 Product Synonym Names    Avian erythroblastic leukemia viral (v erb b) oncogene homolog; Cell growth inhibiting protein 40; Cell proliferation inducing protein 61; EGF R; EGFR; EGFR_HUMAN; Epidermal growth factor receptor (avian erythroblastic leukemia viral (v erb b) oncogene homolog); Epidermal growth factor receptor (erythroblastic leukemia viral (v erb b) oncogene homolog avian); Epidermal growth factor receptor; erbb 1; Erbb; Erbb1; ERBB1; Errp; HER1; mENA; Oncogene ERBB; PIG61; Proto-oncogene c-ErbB-1; Receptor tyrosine protein kinase ErbB 1; Receptor tyrosine-protein kinase ErbB-1; Urogastrone; wa2; Wa5
 Product Gene Name   

anti-EGF antibody

[Similar Products]
 Product Synonym Gene Name    ERBB; HER1; mENA; ERBB1; PIG61 [Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 OMIM    X00588 mRNA
 3D Structure    ModBase 3D Structure for P00533
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 Clonality    Monoclonal
 Isotype    IgG1
 Clone Number    5E10-G10-E11
 Host    Mouse
 Species Reactivity    Human, Monkey
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 Purity/Purification    Affinity Purified
 Form/Format    Purified mouse monoclonal antibody in PBS (pH 7.4) containing with 0.02% sodium azide and 50% glycerol.
 Concentration    0.5mg/ml (lot specific)
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 Source    Human
 Immunogen    Purified recombinant human EGFR protein fragments expressed in E Coli.
 Sensitivity    The antibody detects endogenous level of total EGFR and does not cross-react with related proteins.
 Sequence Similarities    Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily. Contains 1 protein kinase domain.
 Subunit Structure    Binding of the ligand triggers homo- and/or heterodimerization of the receptor triggering its autophosphorylation. Heterodimer with ERBB2. Interacts with ERRFI1; inhibits dimerization of the kinase domain and autophosphorylation. Part of a complex with ERBB2 and either PIK3C2A or PIK3C2B. Interacts with GRB2; an adapter protein coupling the receptor to downstream signaling pathways. Interacts with GAB2; involved in signaling downstream of EGFR. Interacts with STAT3; mediates EGFR downstream signaling in cell proliferation. Interacts with RIPK1; involved in NF-kappa-B activation. Interacts (autophosphorylated) with CBL, CBLB and CBLC; involved in EGFR ubiquitination and regulation. Interacts with SOCS5; regulates EGFR degradation through TCEB1- and TCEB2-mediated ubiquitination and proteasomal degradation. Interacts with PRMT5; methylates EGFR and enhances interaction with PTPN6. Interacts (phosphorylated) with PTPN6; inhibits EGFR-dependent activation of MAPK/ERK. Interacts with COPG1; essential for regulation of EGF-dependent nuclear transport of EGFR by retrograde trafficking from the Golgi to the ER. Interacts with TNK2; this interaction is dependent on EGF stimulation and kinase activity of EGFR. Interacts with PCNA; positively regulates PCNA. Interacts with PELP1. Interacts with MUC1. Interacts with AP2M1. Interacts with FER. May interact with EPS8; mediates EPS8 phosphorylation. Interacts (via SH2 domains) with GRB2, NCK1 and NCK2. Interacts with ATX2. Interacts with GAREM. Interacts (ubiquitinated) with ANKRD13A/B/D; the interaction is direct and may regulate EGFR internalization after EGF stimulation. Interacts with GPER1; the interaction occurs in an estrogen-dependent manner.
 Subcellular Location    Cell membrane; Single-pass type I membrane protein. Endoplasmic reticulum membrane; Single-pass type I membrane protein. Golgi apparatus membrane; Single-pass type I membrane protein. Nucleus membrane; Single-pass type I membrane protein. Endosome. Endoso
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 Other Notes    Small volumes of anti-EGF antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for anti-EGF antibody

   Entrez Summary: The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. Multiple alternatively spliced transcript variants that encode different protein isoforms have been found for this gene. [provided by RefSeq, Jul 2010]
 Product Categories/Family for anti-EGF antibody    Signal Transduction; Protein Phosphorylation; Tyrosine Kinases; Receptor Tyrosine Kinases; Signal Transduction; Growth Factors/Hormones; EGF
 Applications Tested/Suitable for anti-EGF antibody   

Western Blot (WB), Immunocytochemistry (ICC), Immunoprecipitation (IP)

 Application Notes for anti-EGF antibody    WB: 1:1000
ICC: 1:200
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 Western Blot (WB) of anti-EGF antibody    Western blot detection of EGFR in A549, MDA-MB-468 and COS7 cell lysates using EGFR mouse mAb (dilution 1:1000).Predicted band size:134 Kda.Observed band size:175KDa.
anti-EGF antibody Western Blot (WB) (WB) image
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 Immunocytochemistry (ICC) of anti-EGF antibody    Immunocytochemistry staining of HeLa cells using EGFR mouse mAb (dilution 1:200).
anti-EGF antibody Immunocytochemistry (ICC) (ICC) image
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 Immunoprecipitation (IP) of anti-EGF antibody    Immunoprecipitation analysis of Hela cell lysates using EGFR mouse mAb.
anti-EGF antibody Immunoprecipitation (IP) (IP) image
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 Immunocytochemistry (ICC) of anti-EGF antibody    Immunocytochemistry staining of MDA-MB-468 cells fixed with 4% Paraformaldehyde and using EGFR mouse mAb (dilution 1:200).
anti-EGF antibody Immunocytochemistry (ICC) (ICC) image
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NCBI/Uniprot data below describe general gene information for EGF. It may not necessarily be applicable to this product.
 NCBI GI #    29725609
 NCBI GeneID    1956
 NCBI Accession #    NP_005219.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_005228 [Other Products]
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 UniProt Primary Accession #    P00533 [Other Products]
 UniProt Secondary Accession #    O00688; O00732; P06268; Q14225; Q68GS5; Q92795; Q9BZS2; Q9GZX1; Q9H2C9; Q9H3C9; Q9UMD7 [Other Products]
 UniProt Related Accession #    P00533 [Other Products]
 Molecular Weight    175
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 NCBI Official Full Name    epidermal growth factor receptor isoform a
 NCBI Official Synonym Full Names    epidermal growth factor receptor
 NCBI Official Symbol    EGFR [Similar Products]
 NCBI Official Synonym Symbols   
ERBB; HER1; mENA; ERBB1; PIG61; NISBD2
[Similar Products]
 NCBI Protein Information    epidermal growth factor receptor
 UniProt Protein Name    Epidermal growth factor receptor
 UniProt Synonym Protein Names   
Proto-oncogene c-ErbB-1; Receptor tyrosine-protein kinase erbB-1
 Protein Family    Pro-epidermal growth factor
 UniProt Gene Name    EGFR [Similar Products]
 UniProt Synonym Gene Names    ERBB; ERBB1; HER1 [Similar Products]
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 NCBI Summary for EGF    The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. [provided by RefSeq, Jun 2016]
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 UniProt Comments for EGF    EGFR: a receptor tyrosine kinase. This is a receptor for epidermal growth factor (EGF) and related growth factors including TGF-alpha, amphiregulin, betacellulin, heparin-binding EGF-like growth factor, GP30, and vaccinia virus growth factor. EGFR is involved in the control of cell growth and differentiation. It is a single-pass transmembrane tyrosine kinase. Ligand binding to this receptor results in receptor dimerization, autophosphorylation (in trans), activation of various downstream signaling molecules and lysosomal degradation. It can be phosphorylated and activated by Src. Activated EGFR binds the SH2 domain of phospholipase C-gamma (PLC-gamma), activating PLC-gamma-mediated downstream signaling. Phosphorylated EGFR binds Cbl, leading to its ubiquitination and degradation. Grb2 and SHC bind to phospho-EGFR and are involved in the activation of MAP kinase signaling pathways. Phosphorylation on Ser and Thr residues is thought to represent a mechanism for attenuation of EGFR kinase activity. EGFR is overexpressed in breast, head and neck cancers, correlating with poor survival. Activating somatic mutations are seen in lung cancer, corresponding to the minority of patients with strong responses to the EGFR inhibitor Iressa (gefitinib). Mutations and amplifications are also seen in glioblastoma, and upregulation is seen in colon cancer and neoplasms. In xenografts, inhibitors synergize with cytotoxic drugs in the inhibition of many tumor types. Inhibitors include: Iressa/ZD1839, Erbitux, Tarceva, and lapatinib. Four alternatively spliced isoforms have been described.

Protein type: EC 2.7.10.1; EGFR family; Kinase, protein; Membrane protein, integral; Protein kinase, TK; Protein kinase, tyrosine (receptor); TK group; Tumor suppressor

Chromosomal Location of Human Ortholog: 7p11.2

Cellular Component: AP-2 adaptor complex; basolateral plasma membrane; cell surface; cell-cell adherens junction; cytoplasm; early endosome membrane; endosome; endosome membrane; focal adhesion; lipid raft; membrane; nucleus; plasma membrane; receptor complex

Molecular Function: actin filament binding; chromatin binding; enzyme binding; epidermal growth factor receptor activity; identical protein binding; nitric-oxide synthase regulator activity; phosphatidylinositol-4,5-bisphosphate 3-kinase activity; protein binding; protein heterodimerization activity; protein phosphatase binding; protein-tyrosine kinase activity; Ras guanyl-nucleotide exchange factor activity; transmembrane receptor activity; transmembrane receptor protein tyrosine kinase activity; ubiquitin protein ligase binding

Biological Process: calcium-dependent phospholipase A2 activation; cell proliferation; cell surface receptor linked signal transduction; cell-cell adhesion; epidermal growth factor receptor signaling pathway; G1/S-specific positive regulation of cyclin-dependent protein kinase activity; learning and/or memory; MAPKKK cascade; negative regulation of apoptosis; negative regulation of epidermal growth factor receptor signaling pathway; negative regulation of protein catabolic process; peptidyl-tyrosine phosphorylation; phosphoinositide-mediated signaling; phospholipase C activation; positive regulation of cell growth; positive regulation of cell migration; positive regulation of cell proliferation; positive regulation of DNA repair; positive regulation of DNA replication; positive regulation of epithelial cell proliferation; positive regulation of MAP kinase activity; positive regulation of nitric oxide biosynthetic process; positive regulation of phosphorylation; positive regulation of protein amino acid phosphorylation; positive regulation of protein kinase B signaling cascade; positive regulation of transcription from RNA polymerase II promoter; protein amino acid autophosphorylation; protein insertion into membrane; regulation of nitric-oxide synthase activity; regulation of peptidyl-tyrosine phosphorylation; regulation of phosphoinositide 3-kinase cascade; signal transduction

Disease: Lung Cancer
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 Research Articles on EGF    1. these data suggest that Lupeol may act as a potent inhibitor of the EGFR signaling in oral squamous cell carcinoma and therefore imply its role in triggering antitumor efficacy.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-EGF antibodyPathways associated with anti-EGF antibody
 Reference Product  PubMed Publications
 EGF antibody  >4897 publications with EGF and EGF
 ERBB2 antibody  >2515 publications with EGF and ERBB2
 MET antibody  >673 publications with EGF and MET
 MAPK1 antibody  >421 publications with EGF and MAPK1
 STAT3 antibody  >348 publications with EGF and STAT3
 ERBB3 antibody  >332 publications with EGF and ERBB3
 CTNNB1 antibody  >271 publications with EGF and CTNNB1
 GRB2 antibody  >229 publications with EGF and GRB2
 ERBB4 antibody  >224 publications with EGF and ERBB4
 CBL antibody  >221 publications with EGF and CBL
 Products by Pathway  Pathway Diagram
 AGE/RAGE Pathway antibodies  AGE/RAGE Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 Adherens Junction Pathway antibodies  Adherens Junction Pathway Diagram
 Adherens Junction Pathway antibodies  Adherens Junction Pathway Diagram
 AhR Pathway antibodies  AhR Pathway Diagram
 Alpha6-Beta4 Integrin Signaling Pathway antibodies  Alpha6-Beta4 Integrin Signaling Pathway Diagram
 Androgen Receptor Signaling Pathway antibodies  Androgen Receptor Signaling Pathway Diagram
 Arf6 Signaling Events Pathway antibodies  Arf6 Signaling Events Pathway Diagram
 Axon Guidance Pathway antibodies  Axon Guidance Pathway Diagram
 Bladder Cancer Pathway antibodies  Bladder Cancer Pathway Diagram
Diseases associated with anti-EGF antibodyOrgans/Tissues associated with anti-EGF antibody
 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >13105 publications with EGF and Neoplasms
 Carcinoma Antibodies  >7383 publications with EGF and Carcinoma
 Lung Neoplasms Antibodies  >3848 publications with EGF and Lung Neoplasms
 Adenocarcinoma Antibodies  >2924 publications with EGF and Adenocarcinoma
 Kidney Diseases Antibodies  >2669 publications with EGF and Kidney Diseases
 Small Cell Lung Carcinoma Antibodies  >2587 publications with EGF and Small Cell Lung Carcinoma
 Carcinoma, Non-Small-Cell Lung Antibodies  >2553 publications with EGF and Carcinoma, Non-Small-Cell Lung
 Breast Neoplasms Antibodies  >2207 publications with EGF and Breast Neoplasms
 Disease Progression Antibodies  >2145 publications with EGF and Disease Progression
 Carcinoma, Squamous Cell Antibodies  >2042 publications with EGF and Carcinoma, Squamous Cell
 Organ/Tissue Name  Pubmed Publications
 Lung Antibodies  >5123 publications with EGF and Lung
 Kidney Antibodies  >3722 publications with EGF and Kidney
 Vascular Antibodies  >1971 publications with EGF and Vascular
 Brain Antibodies  >1600 publications with EGF and Brain
 Skin Antibodies  >979 publications with EGF and Skin
 Liver Antibodies  >953 publications with EGF and Liver
 Heart Antibodies  >806 publications with EGF and Heart
 Prostate Antibodies  >571 publications with EGF and Prostate
 Muscle Antibodies  >556 publications with EGF and Muscle
 Stomach Antibodies  >403 publications with EGF and Stomach
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