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anti-EIF2AK3 antibody :: Mouse EIF2AK3 Monoclonal Antibody

Scan QR to view Datasheet Catalog #    MBS9217062
Western Blot (WB)
Unit / Price
0.05 mL  /  $155 +1 FREE 8GB USB
0.2 mL  /  $340 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

EIF2AK3, Monoclonal Antibody

★Popular Item★
 Also Known As   

EIF2AK3 Antibody

 Product Synonym Names    Eukaryotic translation initiation factor 2-alpha kinase 3; 2.7.11.1; PRKR-like endoplasmic reticulum kinase; Pancreatic eIF2-alpha kinase; HsPEK; EIF2AK3; PEK; PERK
 Product Gene Name   

anti-EIF2AK3 antibody

[Similar Products]
 Product Synonym Gene Name    PEK; PERK [Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 OMIM    226980
 3D Structure    ModBase 3D Structure for Q9NZJ5
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 Clonality    Monoclonal
 Isotype    IgG1
 Clone Number    1385CT582.4.39
 Host    Mouse
 Species Reactivity    Human, Mouse, Rat
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 Specificity    This EIF2AK3 antibody is generated from a mouse immunized with a recombinant protein conjugated synthetic peptide between 530-850 amino acids from human EIF2AK3.
 Form/Format    Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
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 Cellular Location    Endoplasmic reticulum membrane; Single-pass type I membrane protein.
 Function    Metabolic-stress sensing protein kinase that phosphorylates the alpha subunit of eukaryotic translation initiation factor 2 (eIF-2-alpha/EIF2Sl) on 'Ser-52' during the unfolded protein response (UPR) and in response to low amino acid availability. Converts phosphorylated eIF-2-alpha/EIF2Sl either in a global protein synthesis inhibitor, leading to a reduced overall utilization of amino acids, or to a translation initiation activator of specific mRNAs, such as the transcriptional activator ATF4, and hence allowing ATF4-mediated reprogramming of amino acid biosynthetic gene expression to alleviate nutrient depletion. Serves as a critical effector of unfolded protein response (UPR)-induced Gl growth arrest due to the loss of cyclin-Dl (CCNDl). Involved in control of mitochondrial morphology and function.
 Tissue Location    Ubiquitous. A high level expression is seen in secretory tissues
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 Preparation and Storage    Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
 Other Notes    Small volumes of anti-EIF2AK3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Product Description specifically for anti-EIF2AK3 antibody

   Phosphorylates the alpha subunit of eukaryotic translation-initiation factor 2 (EIF2), leading to its inactivation and thus to a rapid reduction of translational initiation and repression of global protein synthesis. Serves as a critical effector of unfolded protein response (UPR)-induced G1 growth arrest due to the loss of cyclin-D1(CCND1). Involved in control of mitochondrial morphology and function (By similarity).
 Product Categories/Family for anti-EIF2AK3 antibody    New Antibodies; Primary Antibodies; Metabolism
 Applications Tested/Suitable for anti-EIF2AK3 antibody   

Western Blot (WB), ELISA (EIA)

 Application Notes for anti-EIF2AK3 antibody    WB:~~1:250-1:1000
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 Western Blot (WB) of anti-EIF2AK3 antibody    All lanes : Anti-EIF2AK3 Antibody at 1:250-1:1000 dilution
Lane 1: A549 whole cell lysate
Lane 2: PC-12 whole cell lysate
Lane 3: mouse brain lysate
Lane 4: mouse liver lysate
Lysates/proteins at 20 ug per lane.
Secondary
Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 125 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
anti-EIF2AK3 antibody Western Blot (WB) (WB) image
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NCBI/Uniprot data below describe general gene information for EIF2AK3. It may not necessarily be applicable to this product.
 NCBI GI #    134304838
 NCBI GeneID    9451
 NCBI Accession #    NP_004827.4 [Other Products]
 NCBI GenBank Nucleotide #    NM_004836.6 [Other Products]
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 UniProt Primary Accession #    Q9NZJ5 [Other Products]
 UniProt Secondary Accession #    O95846; Q53QY0; Q53SB1; A0AVH1; A0AVH2; B2RCU9 [Other Products]
 UniProt Related Accession #    Q9NZJ5 [Other Products]
 Molecular Weight    125216 Da
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 NCBI Official Full Name    eukaryotic translation initiation factor 2-alpha kinase 3 isoform 1
 NCBI Official Synonym Full Names    eukaryotic translation initiation factor 2 alpha kinase 3
 NCBI Official Symbol    EIF2AK3 [Similar Products]
 NCBI Official Synonym Symbols   
PEK; WRS; PERK
[Similar Products]
 NCBI Protein Information    eukaryotic translation initiation factor 2-alpha kinase 3
 UniProt Protein Name    Eukaryotic translation initiation factor 2-alpha kinase 3
 UniProt Synonym Protein Names   
PRKR-like endoplasmic reticulum kinase; Pancreatic eIF2-alpha kinase; HsPEK
 Protein Family    Eukaryotic translation initiation factor 2-alpha kinase
 UniProt Gene Name    EIF2AK3 [Similar Products]
 UniProt Synonym Gene Names    PEK; PERK; HsPEK [Similar Products]
 UniProt Entry Name    E2AK3_HUMAN
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 NCBI Summary for EIF2AK3    The protein encoded by this gene phosphorylates the alpha subunit of eukaryotic translation-initiation factor 2, leading to its inactivation, and thus to a rapid reduction of translational initiation and repression of global protein synthesis. This protein is thought to modulate mitochondrial function. It is a type I membrane protein located in the endoplasmic reticulum (ER), where it is induced by ER stress caused by malfolded proteins. Mutations in this gene are associated with Wolcott-Rallison syndrome. [provided by RefSeq, Sep 2015]
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 UniProt Comments for EIF2AK3    PERK: a transmembrane protein kinase of the PEK family resident in the endoplasmic reticulum (ER) membrane and is linked to insulin processing. Couples ER stress to translation inhibition. Stress induces autophosphorylation of its kinase domain and increases its activity. Phosphorylates the alpha subunit of eukaryotic translation-initiation factor 2 (eIF2alpha), leading to its inactivation and thus to a rapid reduction of translational initiation and repression of global protein synthesis. A critical effector of unfolded protein response (UPR)-induced G1 growth arrest due to the loss of cyclin d1. Forms dimers with BiP in resting cells. Oligomerizes in ER-stressed cells. LOF mutations cause Wolcott-Rallison syndrome (WRS), characterized by insulin-dependent diabetes in early infancy and, later, multiple system abnormalities. Neuronal death in Alzheimer?s and Parkinson?s diseases is thought to be due to ER stress and has been weakly linked to PEK.

Protein type: Kinase, protein; Translation; Membrane protein, integral; Protein kinase, Ser/Thr (non-receptor); Protein kinase, Other; EC 2.7.11.1; Other group; PEK family; PEK subfamily

Chromosomal Location of Human Ortholog: 2p12

Cellular Component: cytoplasm; endoplasmic reticulum; endoplasmic reticulum membrane; integral to endoplasmic reticulum membrane; membrane; perinuclear region of cytoplasm

Molecular Function: ATP binding; enzyme binding; eukaryotic translation initiation factor 2alpha kinase activity; Hsp90 protein binding; identical protein binding; protein binding; protein homodimerization activity; protein kinase activity; protein phosphatase binding; protein serine/threonine kinase activity

Biological Process: angiogenesis; bone mineralization; calcium-mediated signaling; caspase activation; cellular response to amino acid starvation; cellular response to glucose starvation; chondrocyte development; endocrine pancreas development; endoplasmic reticulum organization and biogenesis; ER overload response; insulin secretion; insulin-like growth factor receptor signaling pathway; negative regulation of myelination; negative regulation of translation; negative regulation of translation initiation in response to stress; ossification; peptidyl-serine phosphorylation; positive regulation of transcription from RNA polymerase I promoter; protein amino acid autophosphorylation; protein amino acid phosphorylation; protein homooligomerization; skeletal development; unfolded protein response

Disease: Epiphyseal Dysplasia, Multiple, With Early-onset Diabetes Mellitus
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Product References and Citations for anti-EIF2AK3 antibody

   Shi Y.,et al.J. Biol. Chem. 274:5723-5730 (1999). Sood R.,et al.Biochem. J. 346:281-293 (2000). Delepine M.,et al.Nat. Genet. 25:406-409 (2000). Ota T.,et al.Nat. Genet. 36:40-45 (2004). Hillier L.W.,et al.Nature 434:724-731 (2005).
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 Research Articles on EIF2AK3    1. In human alveolar epithelial A549 cells, Lipopolysaccharide induces autophagic cell death that depends on the activation of the PERK branch of the unfolded protein response upon endoplasmic reticulum (ER) stress .
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-EIF2AK3 antibodyPathways associated with anti-EIF2AK3 antibody
 Reference Product  PubMed Publications
 HSPA5 antibody  >2 publications with EIF2AK3 and HSPA5
 EIF2S1 antibody  >1 publications with EIF2AK3 and EIF2S1
 DNAJC3 antibody  >1 publications with EIF2AK3 and DNAJC3
 Products by Pathway  Pathway Diagram
 Alzheimer's Disease Pathway antibodies  Alzheimer's Disease Pathway Diagram
 Alzheimer's Disease Pathway antibodies  Alzheimer's Disease Pathway Diagram
 Alzheimers Disease Pathway antibodies  Alzheimers Disease Pathway Diagram
 Epstein-Barr Virus Infection Pathway antibodies  Epstein-Barr Virus Infection Pathway Diagram
 Epstein-Barr Virus Infection Pathway antibodies  Epstein-Barr Virus Infection Pathway Diagram
 Hepatitis C Pathway antibodies  Hepatitis C Pathway Diagram
 Hepatitis C Pathway antibodies  Hepatitis C Pathway Diagram
 Herpes Simplex Infection Pathway antibodies  Herpes Simplex Infection Pathway Diagram
 Herpes Simplex Infection Pathway antibodies  Herpes Simplex Infection Pathway Diagram
 Influenza A Pathway antibodies  Influenza A Pathway Diagram
Diseases associated with anti-EIF2AK3 antibodyOrgans/Tissues associated with anti-EIF2AK3 antibody
 Disease Name  Pubmed Publications
 Wolcott-Rallison syndrome Antibodies  >28 publications with EIF2AK3 and Wolcott-Rallison syndrome
 Nervous System Diseases Antibodies  >6 publications with EIF2AK3 and Nervous System Diseases
 Liver Diseases Antibodies  >5 publications with EIF2AK3 and Liver Diseases
 Brain Diseases Antibodies  >5 publications with EIF2AK3 and Brain Diseases
 Supranuclear Palsy, Progressive Antibodies  >3 publications with EIF2AK3 and Supranuclear Palsy, Progressive
 Drug Toxicity Antibodies  >2 publications with EIF2AK3 and Drug Toxicity
 Renal Insufficiency Antibodies  >2 publications with EIF2AK3 and Renal Insufficiency
 Hepatitis Antibodies  >2 publications with EIF2AK3 and Hepatitis
 Atrophy Antibodies  >1 publications with EIF2AK3 and Atrophy
 Cognition Disorders Antibodies  >1 publications with EIF2AK3 and Cognition Disorders
 Organ/Tissue Name  Pubmed Publications
 Pancreas Antibodies  >16 publications with EIF2AK3 and Pancreas
 Bone Antibodies  >13 publications with EIF2AK3 and Bone
 Liver Antibodies  >11 publications with EIF2AK3 and Liver
 Brain Antibodies  >8 publications with EIF2AK3 and Brain
 Blood Antibodies  >5 publications with EIF2AK3 and Blood
 Heart Antibodies  >2 publications with EIF2AK3 and Heart
 Intestine Antibodies  >1 publications with EIF2AK3 and Intestine
 Skin Antibodies  >1 publications with EIF2AK3 and Skin
 Muscle Antibodies  >1 publications with EIF2AK3 and Muscle
 Thyroid Antibodies  >1 publications with EIF2AK3 and Thyroid
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