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anti-ENO1 antibody :: Mouse anti-Human ENO1 / Alpha Enolase Monoclonal Antibody

Scan QR to view Datasheet Catalog #    MBS246171
Unit / Price
0.05 mg  /  $555 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

ENO1 / Alpha Enolase, Monoclonal Antibody

 Also Known As   

Mouse Monoclonal [clone 8G8] (IgG1,k) to Human ENO1 / Alpha Enolase

 Product Synonym Names    Anti-ENO1 / Alpha Enolase Antibody (clone 8G8) IHC-plus; ENO1; Alpha Enolase; Alpha enolase like 1; ENO1L1; Enolase 1; MBP-1; MBPB1; MPB1; MYC promoter-binding protein 1; NNE; Phosphopyruvate hydratase; Alpha-enolase; Tau-crystallin; MBP1; MPB-1; C-myc promoter-binding protein; Enolase 1; (alpha); Human ENO1; Alpha Enolase
 Product Gene Name   

anti-ENO1 antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 OMIM    172430
 3D Structure    ModBase 3D Structure for P06733
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 Clonality    Monoclonal
 Isotype    IgG1,k
 Clone Number    8G8
 Host    Mouse
 Species Reactivity    Human
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 Purity/Purification    Protein A Purified
 Form/Format    PBS, pH 7.4. Sourced in Ascites.
 Concentration    0.5 mg/ml (lot specific)
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 Target Species    Human
 Immunogen Description    ENO1 (AAH22545, 1 a.a. ~ 435 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
 Immunogen Type    Recombinant protein
 Immunogen    ENO1 / Alpha Enolase antibody was raised against eNO1 (AAH22545, 1 a.a. ~ 435 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
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 Preparation and Storage    Store at -20 degree C. Aliquot to avoid freeze/thaw cycles.
 Other Notes    Small volumes of anti-ENO1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Product Description specifically for anti-ENO1 antibody

   This gene encodes alpha-enolase, one of three enolase isoenzymes found in mammals. Each isoenzyme is a homodimer composed of 2 alpha, 2 gamma, or 2 beta subunits, and functions as a glycolytic enzyme. Alpha-enolase in addition, functions as a structural lens protein (tau-crystallin) in the monomeric form. Alternative splicing of this gene results in a shorter isoform that has been shown to bind to the c-myc promoter and function as a tumor suppressor.
 Applications Tested/Suitable for anti-ENO1 antibody   

Immunohistochemistry (IHC - Paraffin), Immunofluorescence (IF), Western Blot (WB), ELISA (EIA)

 Application Notes for anti-ENO1 antibody    ELISA, IF (10 ug/ml), IHC-P (5 ug/ml), WB (1:500 - 1:1000)
Usage: Western Blot (Cell lysate) - positive control MCF-7. Western Blot (Transfected lysate) - positive control transfected 293T cell line. Immunofluorescence (10 ug/ml) - positive control HeLa cells. Sandwich ELISA (Recombinant protein).
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 Immunohistochemistry (IHC) of anti-ENO1 antibody    Anti-ENO1 antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
anti-ENO1 antibody Immunohistochemistry (IHC) (IHC) image
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 Immunohistochemistry (IHC) of anti-ENO1 antibody    Anti-ENO1 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
anti-ENO1 antibody Immunohistochemistry (IHC) (IHC) image
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 Immunohistochemistry (IHC) of anti-ENO1 antibody    Anti-ENO1 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
anti-ENO1 antibody Immunohistochemistry (IHC) (IHC) image
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 Immunofluorescence (IF) of anti-ENO1 antibody    Immunofluorescence of monoclonal antibody to ENO1 on HeLa cell (antibody concentration 10 ug/ml).
anti-ENO1 antibody Immunofluorescence (IF) image
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 Western Blot (WB) of anti-ENO1 antibody    Western blot of ENO1 expression in transfected 293T cell line by ENO1 monoclonal antibody clone 8G8.
anti-ENO1 antibody Western Blot (WB) (WB) image
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 Western Blot (WB) of anti-ENO1 antibody    ENO1 monoclonal antibody clone 8G8 Western blot of ENO1 expression in MCF-7.
anti-ENO1 antibody Western Blot (WB) (WB) image
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 ELISA (EIA) of anti-ENO1 antibody    Detection limit for recombinant GST tagged ENO1 is approximately 0.3 ng/ml as a capture antibody.
anti-ENO1 antibody ELISA (EIA) image
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NCBI/Uniprot data below describe general gene information for ENO1. It may not necessarily be applicable to this product.
 NCBI GI #    4503571
 NCBI GeneID    2023
 NCBI Accession #    NP_001419.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001428.3 [Other Products]
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 UniProt Primary Accession #    P06733 [Other Products]
 UniProt Secondary Accession #    P22712; Q16704; Q4TUS4; Q53FT9; Q53HR3; Q658M5; Q6GMP2; Q71V37; Q7Z3V6; Q8WU71; B2RD59 [Other Products]
 UniProt Related Accession #    P06733 [Other Products]
 Molecular Weight    36,928 Da
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 NCBI Official Full Name    alpha-enolase isoform 1
 NCBI Official Synonym Full Names    enolase 1, (alpha)
 NCBI Official Symbol    ENO1 [Similar Products]
 NCBI Official Synonym Symbols   
NNE; PPH; MPB1; ENO1L1
[Similar Products]
 NCBI Protein Information    alpha-enolase; c-myc promoter-binding protein-1; enolase-alpha; tau-crystallin; non-neural enolase; alpha enolase like 1; phosphopyruvate hydratase; plasminogen-binding protein; MYC promoter-binding protein 1; 2-phospho-D-glycerate hydro-lyase
 UniProt Protein Name    Alpha-enolase
 UniProt Synonym Protein Names   
2-phospho-D-glycerate hydro-lyase; C-myc promoter-binding protein; Enolase 1; MBP-1; MPB-1; Non-neural enolase; NNE; Phosphopyruvate hydratase; Plasminogen-binding protein
 Protein Family    Enolase
 UniProt Gene Name    ENO1 [Similar Products]
 UniProt Synonym Gene Names    ENO1L1; MBPB1; MPB1; NNE [Similar Products]
 UniProt Entry Name    ENOA_HUMAN
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 NCBI Summary for ENO1    This gene encodes alpha-enolase, one of three enolase isoenzymes found in mammals. Each isoenzyme is a homodimer composed of 2 alpha, 2 gamma, or 2 beta subunits, and functions as a glycolytic enzyme. Alpha-enolase in addition, functions as a structural lens protein (tau-crystallin) in the monomeric form. Alternative splicing of this gene results in a shorter isoform that has been shown to bind to the c-myc promoter and function as a tumor suppressor. Several pseudogenes have been identified, including one on the long arm of chromosome 1. Alpha-enolase has also been identified as an autoantigen in Hashimoto encephalopathy. [provided by RefSeq, Jan 2011]
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 UniProt Comments for ENO1    ENO1: an abundant cytoplasmic enzyme with 2-phospho-D-glycerate hydro-lyase activity. A target of excess protein nitration in Alzheimer disease (AD) brain. Tau-crystallin, a structural lens protein, is produced from an alternative translation start in the enolase gene.

Protein type: Carbohydrate Metabolism - glycolysis and gluconeogenesis; EC 4.2.1.11; Transcription, coactivator/corepressor; Lyase

Chromosomal Location of Human Ortholog: 1p36.2

Cellular Component: extracellular space; phosphopyruvate hydratase complex; membrane; cytoplasm; plasma membrane; M band; nucleus; cytosol

Molecular Function: protein binding; DNA binding; magnesium ion binding; phosphopyruvate hydratase activity; transcription corepressor activity; transcription factor activity

Biological Process: glycolysis; transcription, DNA-dependent; response to virus; carbohydrate metabolic process; glucose metabolic process; pathogenesis; negative regulation of cell growth; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; gluconeogenesis
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 Research Articles on ENO1    1. Isotypes of anti-citrullinated fibrinogen and alpha-enolase can be found in the serum of children with juvenile idiopathic arthritis of all onset types.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with anti-ENO1 antibodyDiseases associated with anti-ENO1 antibody
 Products by Pathway  Pathway Diagram
 Biosynthesis Of Amino Acids Pathway antibodies  Biosynthesis Of Amino Acids Pathway Diagram
 Biosynthesis Of Amino Acids Pathway antibodies  Biosynthesis Of Amino Acids Pathway Diagram
 Carbon Metabolism Pathway antibodies  Carbon Metabolism Pathway Diagram
 Carbon Metabolism Pathway antibodies  Carbon Metabolism Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 Gluconeogenesis Pathway antibodies  Gluconeogenesis Pathway Diagram
 Gluconeogenesis, Oxaloacetate => Fructose-6P Pathway antibodies  Gluconeogenesis, Oxaloacetate => Fructose-6P Pathway Diagram
 Gluconeogenesis, Oxaloacetate => Fructose-6P Pathway antibodies  Gluconeogenesis, Oxaloacetate => Fructose-6P Pathway Diagram
 Glucose Metabolism Pathway antibodies  Glucose Metabolism Pathway Diagram
 Glycogen Storage Diseases Pathway antibodies  Glycogen Storage Diseases Pathway Diagram
 Disease Name  Pubmed Publications
 Carcinoma Antibodies  >38 publications with ENO1 and Carcinoma
 Nervous System Diseases Antibodies  >20 publications with ENO1 and Nervous System Diseases
 Arthritis, Rheumatoid Antibodies  >14 publications with ENO1 and Arthritis, Rheumatoid
 Adenocarcinoma Antibodies  >13 publications with ENO1 and Adenocarcinoma
 Breast Neoplasms Antibodies  >13 publications with ENO1 and Breast Neoplasms
 Carcinoma, Non-Small-Cell Lung Antibodies  >12 publications with ENO1 and Carcinoma, Non-Small-Cell Lung
 Inflammation Antibodies  >12 publications with ENO1 and Inflammation
 Neoplasms, Experimental Antibodies  >9 publications with ENO1 and Neoplasms, Experimental
 Carcinoma, Squamous Cell Antibodies  >7 publications with ENO1 and Carcinoma, Squamous Cell
 Liver Neoplasms Antibodies  >7 publications with ENO1 and Liver Neoplasms
Organs/Tissues associated with anti-ENO1 antibody
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >50 publications with ENO1 and Blood
 Lung Antibodies  >31 publications with ENO1 and Lung
 Brain Antibodies  >31 publications with ENO1 and Brain
 Liver Antibodies  >17 publications with ENO1 and Liver
 Muscle Antibodies  >15 publications with ENO1 and Muscle
 Eye Antibodies  >12 publications with ENO1 and Eye
 Vascular Antibodies  >12 publications with ENO1 and Vascular
 Kidney Antibodies  >11 publications with ENO1 and Kidney
 Nerve Antibodies  >9 publications with ENO1 and Nerve
 Ovary Antibodies  >8 publications with ENO1 and Ovary
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