|
| Product Name
|
|
EphA1, Monoclonal Antibody
|
| Also Known As
|
|
EphA1 Antibody
|
| Product Synonym Names
|
|
EPH; EPHT; EPHT1
|
| Product Gene Name
|
|
|
[Similar Products]
|
| Research Use Only
|
|
For Research Use Only. Not for use in diagnostic procedures.
|
 TOP
|
|
| OMIM
|
|
179610
|
|
| Clonality
|
|
Monoclonal
|
| Clone Number
|
|
5D2
|
| Host
|
|
Mouse
|
| Species Reactivity
|
|
Human
|
|
|
| Form/Format
|
|
Purified antibody in PBS containing 0.03% sodium azide.
|
| Concentration
|
|
100ug/100ul (lot specific)
|
|
|
| Immunogen
|
|
Purified recombinant fragment of EphA1 expressed in E. Coli.
|
| Species
|
|
Mouse IgG1
|
|
|
| Preparation and Storage
|
|
Store at 4 degree C, for long term storage, store at -20 degree C
|
| Other Notes
|
|
Small volumes of anti-EphA1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
|
|
TOP
|
|
|
|
|
Background: EPH receptor A1 (EphA1), with 976-amino acid protein(about 107 kDa), belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. The Eph subfamily represents the largest group of receptor protein tyrosine kinases identified to date and their ligands, the ephrins,can be subdivided into two major subclasses, ephrin-A and ephrin-B. Interaction of Eph receptor tyrosine kinases with their membrane bound ephrin ligands initiates bidirectional signaling events that regulate cell migratory and adhesive behavior, particularly in the nervous system. They have been implicated in various developmental processes, including axonal guidance, angiogenesis, morphogenesis and carcinogenesis.
|
| Product Categories/Family for anti-EphA1 antibody
|
|
Cancer; Cardiovascular; Cell Biology; Epigenetics & Nuclear Signaling; Developmental Biologys; Immunology; Drug Discovery Products; Metabolism; Neuroscience; Signal Transduction; Stem Cells; Autophagy antibody
|
|
| Applications Tested/Suitable for anti-EphA1 antibody
|
|
Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
|
| Application Notes for anti-EphA1 antibody
|
|
WB: 1/500 - 1/2000, IHC:1/200 - 1/1000, ELISA: Propose dilution 1/10000
Not yet tested in other applications. Determining optimal working dilutions by titration test.
|
|
|
| Testing Data of anti-EphA1 antibody
|
|
Figure 1. Western blot analysis using EphA1 mouse mAb against A549 (1) and Hela (2) cell lysate.
|
|
|
| Testing Data #2 of anti-EphA1 antibody
|
|
Figure 2. Immunohistochemical analysis of paraffin-embedded human ovary carcinoma (left) and breast carcinoma (right), showing cytoplasmic localization using EphA1 mouse mAb with DAB staining.
|
|
TOP
|
|
| NCBI/Uniprot data below describe general gene information for EphA1. It may not necessarily be applicable to this product. |
| NCBI GI #
|
|
221316650
|
| NCBI GeneID
|
|
2041
|
| NCBI Accession #
|
|
NP_005223.4
|
[Other Products]
|
| NCBI GenBank Nucleotide #
|
|
NM_005232.4
|
[Other Products]
|
|
|
| UniProt Secondary Accession #
|
|
Q15405; A1L3V3; B5A966; B5A967
|
[Other Products]
|
| UniProt Related Accession #
|
|
P21709
|
[Other Products]
|
| Molecular Weight
|
|
108kDa
|
|
TOP
|
| NCBI Official Full Name
|
|
ephrin type-A receptor 1
|
| NCBI Official Synonym Full Names
|
|
EPH receptor A1
|
| NCBI Official Symbol
|
|
EPHA1
|
[Similar Products]
|
| NCBI Official Synonym Symbols
|
|
EPH; EPHT; EPHT1
|
[Similar Products]
|
| NCBI Protein Information
|
|
ephrin type-A receptor 1; hEpha1; oncogene EPH; eph tyrosine kinase 1; soluble EPHA1 variant 1; soluble EPHA1 variant 2; tyrosine-protein kinase receptor EPH; erythropoietin-producing hepatoma receptor; erythropoietin-producing hepatoma amplified sequence
|
| UniProt Protein Name
|
|
Ephrin type-A receptor 1
|
| UniProt Synonym Protein Names
|
|
EPH tyrosine kinase; EPH tyrosine kinase 1; Erythropoietin-producing hepatoma receptor; Tyrosine-protein kinase receptor EPH
|
| Protein Family
|
|
Ephrin type-A receptor
|
| UniProt Gene Name
|
|
EPHA1
|
[Similar Products]
|
| UniProt Synonym Gene Names
|
|
EPH; EPHT; EPHT1; hEpha1
|
[Similar Products]
|
| UniProt Entry Name
|
|
EPHA1_HUMAN
|
|
|
| NCBI Summary for EphA1
|
|
This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. This gene is expressed in some human cancer cell lines and has been implicated in carcinogenesis. [provided by RefSeq, Jul 2008]
|
|
|
| UniProt Comments for EphA1
|
|
EphA1: a receptor tyrosine kinase. Receptor for members of the ephrin-A family. Binds with a low affinity to ephrin-A1. The Eph receptor tyrosine kinase family, the largest in the tyrosine kinase group, has fourteen members. They bind membrane-anchored ligands, ephrins, at sites of cell-cell contact, regulating the repulsion and adhesion of cells that underlie the establishment, maintenance, and remodeling of patterns of cellular organization. Eph signals are particularly important in regulating cell adhesion and cell migration during development, axon guidance, homeostasis and disease. EphA receptors bind to GPI-anchored ephrin-A ligands, while EphB receptors bind to ephrin-B proteins that have a transmembrane and cytoplasmic domain. Interactions between EphB receptor kinases and ephrin-B proteins transduce signals bidirectionally, signaling to both interacting cell types. Eph receptors and ephrins also regulate the adhesion of endothelial cells and are required for the remodeling of blood vessels. Misexpressed in several cancers, including upregulation in head and neck cancer, and downregulation in invasive breast cancer cell lines and glioblastoma
Protein type: Protein kinase, tyrosine (receptor); Protein kinase, TK; Kinase, protein; EC 2.7.10.1; Membrane protein, integral; TK group; Eph family
Chromosomal Location of Human Ortholog: 7q34
Cellular Component: integral to plasma membrane; plasma membrane
Molecular Function: transmembrane-ephrin receptor activity; protein kinase binding; ATP binding; protein kinase activity
Biological Process: axon guidance; peptidyl-tyrosine phosphorylation; somatic stem cell maintenance; protein amino acid autophosphorylation; positive regulation of angiogenesis; cell surface receptor linked signal transduction; positive regulation of cell proliferation; positive regulation of stress fiber formation; ephrin receptor signaling pathway; negative regulation of protein kinase activity; angiogenesis; positive regulation of cell-matrix adhesion; negative regulation of cell migration; positive regulation of cell migration
|
|
TOP
|
|
| Research Articles on EphA1
|
|
1. Suggest EphA1 receptor may have roles in carcinogenesis and progression of prostate cancer.
|
|
TOP
|
| Precautions
|
|
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
|
| Disclaimer
|
|
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
|
|
TOP
|
|
| Products associated with anti-EphA1 antibody | Pathways associated with anti-EphA1 antibody |
|
|
|
| Diseases associated with anti-EphA1 antibody | Organs/Tissues associated with anti-EphA1 antibody |
| Disease Name |
Pubmed Publications |
| Alzheimer Disease Antibodies |
>23 publications with EphA1 and Alzheimer Disease |
| Adenocarcinoma Antibodies |
>8 publications with EphA1 and Adenocarcinoma |
| Inflammation Antibodies |
>6 publications with EphA1 and Inflammation |
| Colorectal Neoplasms Antibodies |
>6 publications with EphA1 and Colorectal Neoplasms |
| Carcinoma, Hepatocellular Antibodies |
>4 publications with EphA1 and Carcinoma, Hepatocellular |
| Carcinoma, Squamous Cell Antibodies |
>4 publications with EphA1 and Carcinoma, Squamous Cell |
| Cell Transformation, Neoplastic Antibodies |
>3 publications with EphA1 and Cell Transformation, Neoplastic |
| Neoplasms, Experimental Antibodies |
>3 publications with EphA1 and Neoplasms, Experimental |
| Breast Neoplasms Antibodies |
>3 publications with EphA1 and Breast Neoplasms |
| Disease Models, Animal Antibodies |
>3 publications with EphA1 and Disease Models, Animal |
|
|
|
|
|
TOP
|
Antibody 
