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anti-EphA2 antibody :: Mouse anti-Human, Mouse EphA2 Monoclonal Antibody

Scan QR to view Datasheet Catalog #    MBS200110
Unit / Price
0.05 mL  /  $240 +1 FREE 8GB USB
0.1 mL  /  $315 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

EphA2, Monoclonal Antibody

 Also Known As   

EphA2 antibody

 Product Synonym Names    Ephrin receptor EphA2; epoxide hydrolase 1 microsomal (xenobiotic);
 Product Gene Name   

anti-EphA2 antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 OMIM    M59371 mRNA
 3D Structure    ModBase 3D Structure for P29317
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 Clonality    Monoclonal
 Isotype    IgG2b, k
 Clone Number    3D7
 Host    Mouse
 Species Reactivity    Human, Mouse
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 Purity/Purification    By protein-G affinity chromatography
 Form/Format    Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
 Concentration    1mg/ml (lot specific)
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 Antigen Species    Human
 Immunogen    Recombinant human EphA2 (559-976aa) purified from E. coli
 Clone Note    Anti-human EphA2 mAb, clone 3D7, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human EphA2 protein.
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 Preparation and Storage    Can be stored at 4 degree C.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
 Other Notes    Small volumes of anti-EphA2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for anti-EphA2 antibody

   The Eph family of receptors is the largest known subfamily of receptor tyrosine kinases (RTk). Eph RTks and their ligands, the ephrins, are frequently overexpressed in a variety of cancers and tumor cell lines. One Eph family member, EphA2, is also overexpressed in a wide variety of cancers, including breast, small-cell lung and gastrointestinal cancers, melanomas, and neuroblastomas. The ephrin-Eph interactions are important in development, especially in cell-cell interactions involved in nervous system patterning and possibly in cancer.
 Product Categories/Family for anti-EphA2 antibody    Enzymes & Proteases
 Applications Tested/Suitable for anti-EphA2 antibody   

ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)

 Application Notes for anti-EphA2 antibody    Recommended dilution range for Western blot is 1:500 ~ 1:2,000 and immunohistochemistry analysis is 1:100~200.
Recommended starting dilution for Western blot is 1:500 and Immunohistochemistry is 1:100.
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 Western Blot (WB) of anti-EphA2 antibody    The cell lysates (10ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human EphA2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: 293T cell lysate Lane 2.: EphA2 extracellular domain transfected 293T cell lysate Lane 3.: EphA2 cytoplasmic domain transfected 293T cell lysate
anti-EphA2 antibody Western Blot (WB) (WB) image
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 Immunohistochemistry (IHC) of anti-EphA2 antibody    Paraffin embedded sections of human colon and gastric cancer tissue were incubated with anti-human EphA2 (1:100) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB)
anti-EphA2 antibody Immunohistochemistry (IHC) (IHC) image
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NCBI/Uniprot data below describe general gene information for EphA2. It may not necessarily be applicable to this product.
 NCBI GI #    32967311
 NCBI GeneID    1969
 NCBI Accession #    NP_004422 [Other Products]
 NCBI GenBank Nucleotide #    NM_004431.4 [Other Products]
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 UniProt Primary Accession #    P29317 [Other Products]
 UniProt Secondary Accession #    Q8N3Z2; B5A968 [Other Products]
 UniProt Related Accession #    P29317 [Other Products]
 Molecular Weight    54,305 Da
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 NCBI Official Full Name    ephrin type-A receptor 2 isoform 1
 NCBI Official Synonym Full Names    EPH receptor A2
 NCBI Official Symbol    EPHA2 [Similar Products]
 NCBI Official Synonym Symbols   
ECK; CTPA; ARCC2; CTPP1; CTRCT6
[Similar Products]
 NCBI Protein Information    ephrin type-A receptor 2
 UniProt Protein Name    Ephrin type-A receptor 2
 UniProt Synonym Protein Names   
Epithelial cell kinase; Tyrosine-protein kinase receptor ECK
 Protein Family    Ephrin type-A receptor
 UniProt Gene Name    EPHA2 [Similar Products]
 UniProt Synonym Gene Names    ECK [Similar Products]
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 NCBI Summary for EphA2    This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. This gene encodes a protein that binds ephrin-A ligands. Mutations in this gene are the cause of certain genetically-related cataract disorders.[provided by RefSeq, May 2010]
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 UniProt Comments for EphA2    Receptor tyrosine kinase which binds promiscuously membrane-bound ephrin-A family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Activated by the ligand ephrin-A1/EFNA1 regulates migration, integrin-mediated adhesion, proliferation and differentiation of cells. Regulates cell adhesion and differentiation through DSG1/desmoglein-1 and inhibition of the ERK1/ERK2 (MAPK3/MAPK1, respectively) signaling pathway. May also participate in UV radiation-induced apoptosis and have a ligand-independent stimulatory effect on chemotactic cell migration. During development, may function in distinctive aspects of pattern formation and subsequently in development of several fetal tissues. Involved for instance in angiogenesis, in early hindbrain development and epithelial proliferation and branching morphogenesis during mammary gland development. Engaged by the ligand ephrin-A5/EFNA5 may regulate lens fiber cells shape and interactions and be important for lens transparency development and maintenance. With ephrin-A2/EFNA2 may play a role in bone remodeling through regulation of osteoclastogenesis and osteoblastogenesis.
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Product References and Citations for anti-EphA2 antibody

   Ireton RC, Chen J: (2005) Curr Cancer Drug Tarqets. 5 (3), 149-57; Nakamoto M, Berqemann AD: (2002) Microsc Res Tech. 59 (1), 58-67
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 Research Articles on EphA2    1. Data show that Ephrin B3 was concomitantly expressed with EphA2 and Ephrin A1 with higher Ephrin B3 levels found in non-squamous than in squamous tumors.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-EphA2 antibodyPathways associated with anti-EphA2 antibody
 Reference Product  PubMed Publications
 EPHA1 antibody  >41 publications with EphA2 and EPHA1
 EFNA1 antibody  >21 publications with EphA2 and EFNA1
 EFNB3 antibody  >6 publications with EphA2 and EFNB3
 EFNB1 antibody  >6 publications with EphA2 and EFNB1
 EFNA4 antibody  >5 publications with EphA2 and EFNA4
 EFNA3 antibody  >4 publications with EphA2 and EFNA3
 EFNB2 antibody  >4 publications with EphA2 and EFNB2
 EFNA5 antibody  >3 publications with EphA2 and EFNA5
 EFNA2 antibody  >1 publications with EphA2 and EFNA2
 Products by Pathway  Pathway Diagram
 Arf6 Signaling Events Pathway antibodies  Arf6 Signaling Events Pathway Diagram
 Axon Guidance Pathway antibodies  Axon Guidance Pathway Diagram
 Axon Guidance Pathway antibodies  Axon Guidance Pathway Diagram
 Axon Guidance Pathway antibodies  Axon Guidance Pathway Diagram
 Developmental Biology Pathway antibodies  Developmental Biology Pathway Diagram
 Direct P53 Effectors Pathway antibodies  Direct P53 Effectors Pathway Diagram
 EPH-Ephrin Signaling Pathway antibodies  EPH-Ephrin Signaling Pathway Diagram
 EPH-ephrin Mediated Repulsion Of Cells Pathway antibodies  EPH-ephrin Mediated Repulsion Of Cells Pathway Diagram
 EPHA Forward Signaling Pathway antibodies  EPHA Forward Signaling Pathway Diagram
 EPHA-mediated Growth Cone Collapse Pathway antibodies  EPHA-mediated Growth Cone Collapse Pathway Diagram
Diseases associated with anti-EphA2 antibodyOrgans/Tissues associated with anti-EphA2 antibody
 Disease Name  Pubmed Publications
 Breast Neoplasms Antibodies  >83 publications with EphA2 and Breast Neoplasms
 Lung Neoplasms Antibodies  >63 publications with EphA2 and Lung Neoplasms
 Disease Progression Antibodies  >54 publications with EphA2 and Disease Progression
 Neoplasm Invasiveness Antibodies  >53 publications with EphA2 and Neoplasm Invasiveness
 Neoplasms, Experimental Antibodies  >50 publications with EphA2 and Neoplasms, Experimental
 Lung Diseases Antibodies  >48 publications with EphA2 and Lung Diseases
 Disease Models, Animal Antibodies  >43 publications with EphA2 and Disease Models, Animal
 Ovarian Neoplasms Antibodies  >39 publications with EphA2 and Ovarian Neoplasms
 Inflammation Antibodies  >32 publications with EphA2 and Inflammation
 Carcinoma, Hepatocellular Antibodies  >29 publications with EphA2 and Carcinoma, Hepatocellular
 Organ/Tissue Name  Pubmed Publications
 Vascular Antibodies  >109 publications with EphA2 and Vascular
 Lung Antibodies  >99 publications with EphA2 and Lung
 Brain Antibodies  >85 publications with EphA2 and Brain
 Prostate Antibodies  >60 publications with EphA2 and Prostate
 Liver Antibodies  >48 publications with EphA2 and Liver
 Eye Antibodies  >42 publications with EphA2 and Eye
 Lymph Node Antibodies  >31 publications with EphA2 and Lymph Node
 Embryonic Tissue Antibodies  >29 publications with EphA2 and Embryonic Tissue
 Kidney Antibodies  >24 publications with EphA2 and Kidney
 Skin Antibodies  >23 publications with EphA2 and Skin
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