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anti-EGFR antibody :: anti-Human Epidermal Growth Factor Receptor (EGFR), phospho-Ser 1047 + Positive Control Monoclonal Antibody

Scan QR to view Datasheet Catalog #    MBS311326
Testing Data
Unit / Price
0.1 mg  /  $675 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Epidermal Growth Factor Receptor (EGFR), phospho-Ser 1047 + Positive Control, Monoclonal Antibody

 Also Known As   

MAb to EGFR pS1047

 Product Synonym Names    Monoclonal Antibody to Human Epidermal Growth Factor Receptor (EGFR), phosphorylated at Serine 1047
 Product Gene Name   

anti-EGFR antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Chromosome Location    Chromosome: 7; NC_000007.13 (55086725..55275031). Location: 7p12
 OMIM    131550
 3D Structure    ModBase 3D Structure for P00533
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 Clonality    Monoclonal
 Isotype    IgG1, kappa
 Clone Number    1H9
 Host    Host: Mouse
Source: Cell Culture
 Species Reactivity    Human
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 Specificity    Epidermal Growth Factor Receptor (EGFR), phospho-Ser 1047 + Positive Control
The EGF Receptor (EFR) is activated upon stimulation with growth factors of the EGF family and autophosphorylates at multiple tyrosine residues. Serine phosphorylation of the
 Purity/Purification    Thiophilic adsorption and size exclusion chromatography
 Form/Format    Purified, Lyophilized.
Reconstitute with 1ml water for 15 minutes at room temperature.
 Concentration    100ug/ml (prior to lyophilization) (lot specific)
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 Immunogen    Synthetic phosphopeptide corresponding to amino acid residues surrounding Serine 1047
 Affinity Constant    Not determined
 Buffer    Lyophilized from 2X PBS, PEG and Sucrose
 Preservative    0.09% Sodium Azide
 Lyophilized    Yes
 Important Note    Centrifuge before opening to ensure complete recovery of vial contents.
 Warnings    This product contains sodium azide, which has been classified as Xn (Harmful) in European Directive 67/548/EEC in the concentration range of 0.1 - 1.0 %. When disposing of this reagent through lead or copper plumbing, flush with copious volumes of water to prevent azide build-up in drains.
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 Preparation and Storage    Store lyophilized product at -20 degree C. After reconstitution, aliquot and store at -80 degree C. Thawed aliquots may be stored at 2 to 8 degree C for up to 3 months.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Other Notes    Small volumes of anti-EGFR antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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 Product Categories/Family for anti-EGFR antibody    Monoclonal Antibodies to Cytokines and Growth Factors
 Applications Tested/Suitable for anti-EGFR antibody   

EIA/ELISA, Immunofluorescence Assay, Western Blot

 Application Notes for anti-EGFR antibody    Immunoblotting: 0.5ug/ml for HRP/ECL detection.
Recommended blocking buffer CPPT: 0.5% (w/v) casein, 1% (w/v) PEG 4000, 1% (w/v) Polyvinylpyrrolidone (PVP), 0.1% Tween 20, 10mM Tris/HCl, pH 7.4, 150mM Sodium Chloride
Immunocytochemistry: 0.1-1ug/ml<
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 Testing Data of anti-EGFR antibody    anti-EGFR antibody Testing Data image
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NCBI/Uniprot data below describe general gene information for EGFR. It may not necessarily be applicable to this product.
 NCBI GI #    553271
 NCBI GeneID    1956
 NCBI Accession #    AAA63171.1 [Other Products]
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 UniProt Primary Accession #    P00533 [Other Products]
 UniProt Secondary Accession #    O00688; O00732; P06268; Q14225; Q68GS5; Q92795; Q9BZS2; Q9GZX1; Q9H2C9; Q9H3C9; Q9UMD7 [Other Products]
 UniProt Related Accession #    P00533; Q147T7; Q2TTR7; Q504U8; Q59FL8; Q68GS6; Q75MF2; Q8WZA5; Q9H3C8 [Other Products]
 Molecular Weight    134,277 Da [Similar Products]
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 NCBI Official Full Name    epidermal growth factor receptor
 NCBI Official Synonym Full Names    epidermal growth factor receptor
 NCBI Official Symbol    EGFR [Similar Products]
 NCBI Official Synonym Symbols   
ERBB; HER1; mENA; ERBB1; PIG61
[Similar Products]
 NCBI Protein Information    epidermal growth factor receptor; OTTHUMP00000159661; OTTHUMP00000159662; OTTHUMP00000159663; OTTHUMP00000209210; OTTHUMP00000209211; proto-oncogene c-ErbB-1; cell growth inhibiting protein 40; cell proliferation-inducing protein 61; receptor tyrosine-protein kinase erbB-1; avian erythroblastic leukemia viral (v-erb-b) oncogene homolog
 UniProt Protein Name    Epidermal growth factor receptor
 UniProt Synonym Protein Names   
Proto-oncogene c-ErbB-1; Receptor tyrosine-protein kinase erbB-1
 Protein Family    Epidermal growth factor receptor
 UniProt Gene Name    EGFR [Similar Products]
 UniProt Synonym Gene Names    ERBB1 [Similar Products]
 UniProt Entry Name    EGFR_HUMAN
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 NCBI Summary for EGFR    The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. Multiple alternatively spliced transcript variants that encode different protein isoforms have been found for this gene. [provided by RefSeq]
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 UniProt Comments for EGFR    Function: Cell surface receptor for EGF, but also for other members of the EGF family, such as TGF-alpha, BTC/betacellulin, AREGAREGB/amphiregulin, HBEGF, GP30 and vaccinia virus growth factor. Ligand binding triggers a conformation change, leading to activation of the kinase and subsequent phosphorylation of down-stream protein kinases. Is involved in the control of cell growth, proliferation and differentiation. Phosphorylates MUC1 in breast cancer cells and increases the interaction of MUC1 with SRC and CTNNB1/beta-catenin. Ref.33 Ref.54 Ref.55 Ref.56 Ref.60 Ref.63 Ref.65Isoform 2 may act as an antagonist of EGF action. Ref.33 Ref.54 Ref.55 Ref.56 Ref.60 Ref.63 Ref.65

Catalytic activity: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate. Ref.56 Ref.59 Ref.60 Ref.61 Ref.62 Ref.63

Subunit structure: EGF binding triggers dimerization of the receptor and promotes autophosphorylation. The activate receptor dimer binds one EGF molecule. Heterodimer with ERBB2. Interacts with ERRFI1. Interaction with ERRFI1 inhibits dimerization of the kinase domain and autophosphorylation. Binds RIPK1. Interacts (via autophosphorylated C-terminal tail) with CBL. Part of a complex with ERBB2 and either PIK3C2A or PIK3C2B. Interacts (autophosphorylated form) with PIK3C2B; the interaction may be indirect. Interacts with PELP1. Binds MUC1. Interacts with AP2M1. Interacts with GAB2. Interacts with COPG, upon EGF treatment; interaction is essential for regulation of EGF-dependent nuclear transport of EGFR by retrograde trafficking from the Golgi to the ER. Interacts with TNK2; this interaction is dependent on EGF stimulation and kinase activity of EGFR. Ref.26 Ref.28 Ref.31 Ref.32 Ref.33 Ref.37 Ref.41 Ref.44 Ref.52 Ref.54 Ref.55 Ref.60 Ref.62 Ref.63 Ref.64 Ref.65

Subcellular location: Cell membrane; Single-pass type I membrane protein. Endoplasmic reticulum membrane; Single-pass type I membrane protein. Golgi apparatus membrane; Single-pass type I membrane protein. Nucleus membrane; Single-pass type I membrane protein. Endosome. Note: In response to EGF, translocated from the cell membrane to the nucleus via Golgi and ER. Co-localizes with TNK2 on the endosomes. Ref.41 Ref.52 Ref.54 Ref.63Isoform 2: Secreted Ref.41 Ref.52 Ref.54 Ref.63.

Tissue specificity: Ubiquitously expressed. Isoform 2 is also expressed in ovarian cancers. Ref.40

Post-translational modification: Phosphorylation of Ser-695 is partial and occurs only if Thr-693 is phosphorylated. Ref.17 Ref.25 Ref.30 Ref.34 Ref.35 Ref.36 Ref.38 Ref.39 Ref.42 Ref.43 Ref.45 Ref.46 Ref.47 Ref.48 Ref.50 Ref.51 Ref.59 Ref.60 Ref.62 Ref.63Monoubiquitinated and polyubiquitinated upon EGF stimulation; which does not affect tyrosine kinase activity or signaling capacity but may play a role in lysosomal targeting. Polyubiquitin linkage is mainly through 'Lys-63', but linkage through 'Lys-48', 'Lys-11' and 'Lys-29' also occur.

Involvement in disease: Defects in EGFR are associated with lung cancer (LNCR) [

MIM:211980].

Miscellaneous: Binding of EGF to the receptor leads to dimerization, internalization of the EGF-receptor complex, induction of the tyrosine kinase activity, stimulation of cell DNA synthesis, and cell proliferation.

Sequence similarities: Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily.Contains 1 protein kinase domain.
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 Research Articles on EGFR    1. EGFR was found to be expressed in 61.8% of brain gliomas, with strongly positive expression in 12.2% of them
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-EGFR antibodyPathways associated with anti-EGFR antibody
 Reference Product  PubMed Publications
 EGF antibody  >4510 publications with EGFR and EGF
 ERBB2 antibody  >2276 publications with EGFR and ERBB2
 MET antibody  >534 publications with EGFR and MET
 MAPK1 antibody  >398 publications with EGFR and MAPK1
 ERBB3 antibody  >306 publications with EGFR and ERBB3
 STAT3 antibody  >299 publications with EGFR and STAT3
 CTNNB1 antibody  >232 publications with EGFR and CTNNB1
 GRB2 antibody  >216 publications with EGFR and GRB2
 ERBB4 antibody  >211 publications with EGFR and ERBB4
 CBL antibody  >203 publications with EGFR and CBL
 Products by Pathway  Pathway Diagram
 Adherens Junction Pathway antibodies  Adherens Junction Pathway Diagram
 Adherens Junction Pathway antibodies  Adherens Junction Pathway Diagram
 Alpha6-Beta4 Integrin Signaling Pathway antibodies  Alpha6-Beta4 Integrin Signaling Pathway Diagram
 Androgen Receptor Signaling Pathway antibodies  Androgen Receptor Signaling Pathway Diagram
 Arf6 Signaling Events Pathway antibodies  Arf6 Signaling Events Pathway Diagram
 Axon Guidance Pathway antibodies  Axon Guidance Pathway Diagram
 Bladder Cancer Pathway antibodies  Bladder Cancer Pathway Diagram
 Bladder Cancer Pathway antibodies  Bladder Cancer Pathway Diagram
 Calcium Signaling Pathway antibodies  Calcium Signaling Pathway Diagram
 Calcium Signaling Pathway antibodies  Calcium Signaling Pathway Diagram
Diseases associated with anti-EGFR antibodyOrgans/Tissues associated with anti-EGFR antibody
 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >9975 publications with EGFR and Neoplasms
 Carcinoma Antibodies  >5441 publications with EGFR and Carcinoma
 Lung Neoplasms Antibodies  >2652 publications with EGFR and Lung Neoplasms
 Lung Diseases Antibodies  >2485 publications with EGFR and Lung Diseases
 Adenocarcinoma Antibodies  >2073 publications with EGFR and Adenocarcinoma
 Breast Neoplasms Antibodies  >1765 publications with EGFR and Breast Neoplasms
 Carcinoma, Non-Small-Cell Lung Antibodies  >1763 publications with EGFR and Carcinoma, Non-Small-Cell Lung
 Kidney Diseases Antibodies  >1550 publications with EGFR and Kidney Diseases
 Carcinoma, Squamous Cell Antibodies  >1512 publications with EGFR and Carcinoma, Squamous Cell
 Head and Neck Neoplasms Antibodies  >1305 publications with EGFR and Head and Neck Neoplasms
 Organ/Tissue Name  Pubmed Publications
 Lung Antibodies  >3470 publications with EGFR and Lung
 Kidney Antibodies  >2186 publications with EGFR and Kidney
 Vascular Antibodies  >1437 publications with EGFR and Vascular
 Brain Antibodies  >1203 publications with EGFR and Brain
 Skin Antibodies  >754 publications with EGFR and Skin
 Liver Antibodies  >648 publications with EGFR and Liver
 Heart Antibodies  >503 publications with EGFR and Heart
 Prostate Antibodies  >484 publications with EGFR and Prostate
 Muscle Antibodies  >439 publications with EGFR and Muscle
 Eye Antibodies  >328 publications with EGFR and Eye
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