View our list of available Coronavirus (COVID-19, SARS-CoV-2, 2019-nCOV) antibodies, recombinant proteins, ELISA Kits... Options Full Datasheet Printer Friendly Datasheet Prepare for Printing Full Datasheet Printer Friendly Datasheet Prepare for Printing Have Questions? Ask Us! Request A Quote Today! Add To Compare List Mouse anti-E Coli Escherichia coli, O157 Somatic Antigen Monoclonal Antibody Catalog # MBS602479 Unit / Price 1 mg / $1,240 +1 FREE 8GB USB 5x1 mg / $5,400 +6 FREE 8GB USB Product Name Product Info Accession #s Product Desc Diseases/Tissues/Pathways Applications References Product Name Escherichia coli, O157 Somatic Antigen, Monoclonal Antibody Full Product Name Escherichia coli, O157 Somatic Antigen (E. coli) Product Synonym Names Anti -Escherichia coli, O157 Somatic Antigen (E. coli) Research Use Only For Research Use Only. Not for use in diagnostic procedures. TOP Clonality Monoclonal Isotype IgG2b Clone Number 1.B.250A Host Mouse Species Reactivity E Coli Specificity Specific for E. coli serotype O157. Non-reactive with E. coli serotypes O111, O125, O20, O55 and K12. Purity/Purification Affinity PurifiedPurified by Protein A affinity chromatography. Form/Format Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide. No stabilizing proteins added. Immunogen E. coli (whole cell). Preparation and Storage May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Other Notes Small volumes of anti-Escherichia coli, O157 Somatic Antigen antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply. TOP Related Product Information for anti-Escherichia coli, O157 Somatic Antigen antibody Escherichia coli (E. coli) is a Gram negative bacterium that is commonly found in the lower intestine of warm-blooded organisms (endotherms). Most E. coli strains are harmless, but some, such as serotype O157:H7, can cause serious food poisoning in humans, and are occasionally responsible for costly product recalls.[1][2] The harmless strains are part of the normal flora of the gut, and can benefit their hosts by producing vitamin K2,[3] or by preventing the establishment of pathogenic bacteria within the intestine.[4][5] E. coli are not always confined to the intestine, and their ability to survive for brief periods outside the body makes them an ideal indicator organism to test environmental samples for fecal contamination.[6][7] The bacteria can also be grown easily and its genetics are comparatively simple and easily-manipulated or duplicated through a process of metagenics, making it one of the best-studied prokaryotic model organisms, and an important species in biotechnology and microbiology. E. coli was discovered by German pediatrician and bacteriologist Theodor Escherich in 1885,[6] and is now classified as part of the Enterobacteriaceae family of gamma-proteobacteria.[8]A strain of E. coli is a sub-group within the species that has unique characteristics that distinguish it from other E. coli strains. These differences are often detectable only on the molecular level; however, they may result in changes to the physiology or lifecycle of the bacterium. For example, a strain may gain pathogenic capacity, the ability to use a unique carbon source, the ability to inhabit a particular ecological niche or the ability to resist antimicrobial agents. Different strains of E. coli are often host-specific, making it possible to determine the source of fecal contamination in environmental samples.[6][7] For example, knowing which E. coli strains are present in a water sample allows to make assumptions about whether the contamination originated from a human, another mammal or a bird. Product Categories/Family for anti-Escherichia coli, O157 Somatic Antigen antibody Antibodies; Abs to Infectious Disease E. coli Applications Tested/Suitable for anti-Escherichia coli, O157 Somatic Antigen antibody ELISA (EL/EIA), Immunofluorescence (IF) Application Notes for anti-Escherichia coli, O157 Somatic Antigen antibody Suitable for use in ELISA and Immunofluorescence.Dilution: Immunofluorescence: 1:10-1:50ELISA: 1:20-1:200 TOP Precautions All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines. Disclaimer While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information. TOP