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anti-F4/80 antibody :: Rat F4/80 Monoclonal Antibody

Scan QR to view Datasheet Catalog #    MBS216411
Unit / Price
0.025 mg  /  $165 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

F4/80, Monoclonal Antibody

 Also Known As   

RAT ANTI MOUSE F4/80:Biotin

 Product Gene Name   

anti-F4/80 antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Clonality    Monoclonal
 Isotype    IgG2b
 Clone Number    Cl:A3-1
 Host    Rat
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 Form/Format    Biotin
Purified IgG conjugated to Biotin - liquid
 Concentration    IgG concentration 0.1 mg/ml (lot specific)
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 Perservative Stabilisers    0.09% Sodium Azide
1% Bovine Serum Albumin
Preparation
 Buffer Solution    Phosphate buffered saline
 Target Species    Mouse
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 Preparation and Storage    Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.
 Other Notes    Small volumes of anti-F4/80 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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 Applications Tested/Suitable for anti-F4/80 antibody   

Flow cytometry (FC/FACS)

 Application Notes for anti-F4/80 antibody    Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry: Minimum Dilution: Neat; Maximum Dilution: 1/10
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 Testing Data of anti-F4/80 antibody    Staining of mouse peritoneal macrophages with Rat anti Mouse F4/80 antigen:FITC (MBS216414)
anti-F4/80 antibody Testing Data image
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 Testing Data of anti-F4/80 antibody    Immunofluorescence image of mouse small intestine stained with Rat anti Mouse F4/80 (MBS216424), red and Rat anti Mouse CD45 , green; nuclei are stained with DAPI, blue. The image higlights macrophages within the lamina propria
anti-F4/80 antibody Testing Data image
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 Testing Data of anti-F4/80 antibody    Staining of mouse peritoneal macrophages cells with Rat anti Mouse F4/80 antigen:APC (MBS216408)
anti-F4/80 antibody Testing Data image
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 Testing Data of anti-F4/80 antibody    Staining of mouse peritoneal macrophages with Rat anti Mouse F4/80 antigen: RPE - Alexa Fluor 750 (MBS219371P750)
anti-F4/80 antibody Testing Data image
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 Testing Data of anti-F4/80 antibody    Staining of J774 cells with Rat anti Mouse F4/80 antigen:Low Endotoxin (MBS216412)
anti-F4/80 antibody Testing Data image
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 Testing Data of anti-F4/80 antibody    Frozen mouse spleen stained with A: Rat anti Mouse F4/80 (MBS219371) followed by Goat anti Rat IgG:HRP (MBS235195) or B: Rat anti Mouse F4/80 (MBS219371) preincubated with 2 molar excess of Human anti Idiotypic MBS219371 (HCA154) followed by Goat anti Rat IgG:HRP (MBS235195)
anti-F4/80 antibody Testing Data image
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 Testing Data of anti-F4/80 antibody    Staining of J774 cells with Rat anti Mouse F4/80 antigen:Biotin (MBS216409)
anti-F4/80 antibody Testing Data image
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 Testing Data of anti-F4/80 antibody    Staining of mouse peritoneal macrophages with Rat anti Mouse F4/80 antigen: Pacific Blue(MBS219371PB)
anti-F4/80 antibody Testing Data image
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 Testing Data of anti-F4/80 antibody    Published customer image: Post-injury 7,8-dihydroxyflavone treatment increased brain BDNF levels and promoted CREB activation. (A) Bar graphs demonstrating brain derived neurotrophic factor (BDNF) protein concentrations measured by enzyme-linked immunosorbent assay (ELISA) in sham-injured, vehicle-treated, and 20 mg/kg 7,8-dihydroxyflavone (DHF 20)-treated mice at 4 days post-TBI. DHF 20 significantly increased BDNF protein levels in the ipsilateral hemisphere at 4 days post-injury. (B) Bar graphs demonstrating BDNF mRNA expression measured by real -time quantitative reverse transcriptase polymerase chain reaction (RT-PCR) at 1 and 4 days post-injury. DHF 20 significantly increased BDNF mRNA levels in the ipsilateral hemisphere at 4 days post-injury. (C) Western blot analysis of the phospho-CREB level in the ipsilateral hemisphere of sham-injured, vehicle-treated, and DHF 20-treated mice at 1 h, 1 day, and 4 days post-injury. DHF 20 enhanced CREB phosphorylation at 4 days post-injury. (D) Identification of phospho-CREB-positive cells 4 day post-injury in the peri-contusional margin by double immunofluorescence staining. Phospho-CREB is shown in red, and NeuN (neurons), GFAP (astrocytes), or F4/80 (microglia) is shown in green. Co-localization of phospho-CREB with NeuN is shown by yellow labeling. Sections were stained with DAPI (blue) to show all nuclei. Values are mean +/- SEM; *P
anti-F4/80 antibody Testing Data image
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 Testing Data of anti-F4/80 antibody    Staining of J774 cells with Rat anti Mouse F4/80 antigen Biotin (MBS216410)
anti-F4/80 antibody Testing Data image
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NCBI/Uniprot data below describe general gene information for F4/80. It may not necessarily be applicable to this product.
 NCBI GI #    33859546
 NCBI GeneID    13733
 NCBI Accession #    NP_034260.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_010130.4 [Other Products]
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 UniProt Related Accession #    Q61549 [Other Products]
 Molecular Weight    102,130 Da
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 NCBI Official Full Name    EGF-like module-containing mucin-like hormone receptor-like 1
 NCBI Official Synonym Full Names    EGF-like module containing, mucin-like, hormone receptor-like sequence 1
 NCBI Official Symbol    Emr1 [Similar Products]
 NCBI Official Synonym Symbols   
Ly71; F4/80; Gpf480; TM7LN3; DD7A5-7; EGF-TM7
[Similar Products]
 NCBI Protein Information    EGF-like module-containing mucin-like hormone receptor-like 1; EGF-like module receptor 1; EMR1 hormone receptor; cell surface glycoprotein F4/80; lymphocyte antigen 71
 UniProt Protein Name    EGF-like module-containing mucin-like hormone receptor-like 1
 UniProt Synonym Protein Names   
Cell surface glycoprotein F4/80; EGF-like module receptor 1; EMR1 hormone receptor
 UniProt Gene Name    Emr1 [Similar Products]
 UniProt Synonym Gene Names    Gpf480 [Similar Products]
 UniProt Entry Name    EMR1_MOUSE
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 UniProt Comments for F4/80    EMR1: Could be involved in cell-cell interactions. Belongs to the G-protein coupled receptor 2 family. LN-TM7 subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, multi-pass; GPCR, family 2; Receptor, GPCR; Membrane protein, integral

Cellular Component: membrane; plasma membrane; integral to membrane; external side of plasma membrane

Molecular Function: G-protein coupled receptor activity; signal transducer activity; transmembrane receptor activity; calcium ion binding

Biological Process: G-protein coupled receptor protein signaling pathway; cell surface receptor linked signal transduction; signal transduction
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 Research Articles on F4/80    1. EMR1 expression is restricted to mature blood and tissue eosinophils. Targeting of eosinophils with afucosylated anti-EMR1 antibody shows promise as a treatment for eosinophilic disorders.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with anti-F4/80 antibodyDiseases associated with anti-F4/80 antibody
 Products by Pathway  Pathway Diagram
 GPCRs, Class B Secretin-like Pathway antibodies  GPCRs, Class B Secretin-like Pathway Diagram
 Non-odorant GPCRs Pathway antibodies  Non-odorant GPCRs Pathway Diagram
 Disease Name  Pubmed Publications
 Inflammation Antibodies  >31 publications with F4/80 and Inflammation
 Insulin Resistance Antibodies  >15 publications with F4/80 and Insulin Resistance
 Necrosis Antibodies  >8 publications with F4/80 and Necrosis
 Liver Diseases Antibodies  >7 publications with F4/80 and Liver Diseases
 Fibrosis Antibodies  >6 publications with F4/80 and Fibrosis
 Fatty Liver Antibodies  >6 publications with F4/80 and Fatty Liver
 Cardiovascular Diseases Antibodies  >4 publications with F4/80 and Cardiovascular Diseases
 Atherosclerosis Antibodies  >3 publications with F4/80 and Atherosclerosis
 Weight Gain Antibodies  >3 publications with F4/80 and Weight Gain
 Weight Loss Antibodies  >2 publications with F4/80 and Weight Loss
Organs/Tissues associated with anti-F4/80 antibody
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >31 publications with F4/80 and Blood
 Connective Tissue Antibodies  >15 publications with F4/80 and Connective Tissue
 Liver Antibodies  >12 publications with F4/80 and Liver
 Muscle Antibodies  >6 publications with F4/80 and Muscle
 Spleen Antibodies  >4 publications with F4/80 and Spleen
 Bone Antibodies  >3 publications with F4/80 and Bone
 Bone Marrow Antibodies  >3 publications with F4/80 and Bone Marrow
 Embryonic Tissue Antibodies  >2 publications with F4/80 and Embryonic Tissue
 Kidney Antibodies  >2 publications with F4/80 and Kidney
 Lung Antibodies  >2 publications with F4/80 and Lung
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